CD98 Antibody (UM7F8)

Immunocytochemistry/Immunofluorescence: CD98 Antibody (UM7F8) [NBP2-34291] - Analysis of CD89 antibody at 1:200 dilution. Fixation in 4% PAF and experiment done without permeabilization. Primary antibody (1/200) in blocking buffer, overnight @4°C. Secondary AB anti-Mouse IgG - AF594 1/600, 1h, R.T. Confocal microscopy. Image from verified customer review.

• Reactivity: Hu
• Applications: Flow, ICC/IF
• Clone: UM7F8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CD98 Antibody (UM7F8) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34506)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Molt 13 T cell line
• Localization: Cell surface, cytoplasmic
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC3A2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
• Application Notes: Comitogenic with soluble anti-CD2 and immobilized anti-CD3 mAbs.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD98 Antibody (UM7F8)

• 4F2
• 4F2hc
• 4T2HC
• antigen identified by monoclonal antibodies 4F2, TRA1.10, TROP4, and T43,4F2HC
• CD98 antigen
• CD98 heavy chain
• CD98
• CD98HC
• CH98hc
• FRP-1
• heavy chain
• Lymphocyte activation antigen 4F2 large subunit
• MDU1antigen defined by monoclonal 4F2, heavy chain
• monoclonal 44D7
• NACAE4F2 cell-surface antigen heavy chain
• SLC3A2
• solute carrier family 3 (activators of dibasic and neutral amino acidtransport), member 2,4F2 heavy chain antigen

Background

The CD98 gene is a member of the solute carrier family and encodes a cell surface, transmembrane protein with an alpha amylase domain. The protein exists as the heavy chain of a heterodimer, covalently bound through di-sulfide bonds to one of several possible

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for CD98 Antibody (NBP2-34291) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IF Human 04/17/2023

Summary

• Application: Immunofluorescence
• Sample Tested: HCT-116 human colorectal carcinoma cell line,hela cell,HT-29 colon cancer cell line
• Species: Human
• Lot: 6520-1P81210

Comments

• Comments: fixation in 4% PAF
  no permeabilisation
  primary AB 1/200 in blocking buffer, o.night @4°C
  secondary AB anti-Mouse IgG -AF594 1/600, 1h, R.T.
  Confocal microscopy

Bioinformatics

• Gene Symbol: SLC3A2
• Uniprot:
  • P08195()
  • P08195()
  • P08195()