CD98 Antibody (HBJ127) - Azide and BSA Free

Immunocytochemistry /Immunofluorescence: CD98 Antibody (HBJ127) - Azide and BSA Free [NBP2-81108] - Immunofluorescence staining of fixed HeLa cells with [NBP2-81108]. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with NBP2-81108 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81108, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu
• Applications: ELISA, Func, ICC/IF, B/N
• Clone: HBJ127
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CD98 Antibody (HBJ127) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse CD98 Antibody (HBJ127) - Azide and BSA Free (NBP2-81108) is a recombinant monoclonal antibody validated for use in ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: HBJ127 was obtained from hybridomas generated by the fusion of mouse myeloma cells and spleen cells from mice which had been immunized with T24 hyman bladder cancer cells.
• Specificity: HBJ127 binds to the CD98 heavy chain at an epitope consisting of residues AFS (aa 442-444) (Itoh et al, 2007). The antibody has not been shown to cross-react with other species. CD98 is expressed at high levels on monocytes and at very low level on peripheral blood T and B lymphocytes, splenocytes, NK cells and granulocytes. CD98 is a disulfide-linked and glycosylated type II integral membrane protein. This protein has roles in normal and neoplastic cell growth and is required for the function of light chain amino acid transporters. It is also involved in sodium-independent transport of large neitral amino acids and in guiding and targeting LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7, CD98 acts as an arginine/glutamine exchanger.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLC3A2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • ELISA
  • Functional
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: HBJ127 can inhibit lymphocyte activation and proliferation in vitro (Yagita and Hashimoto, 1986), as well as inhibit the growth of human bladder tumors (Yagita, 1986). The antibody also mediates the inhibition of DNA synthesis of Con A-stimulated lymphocytes at concentrations greater than 13 ug/ml, the inhibition of mAB 6-1-13-induced c-src expression and the inhibition of mAb 6-1-13-induced polykaryocyte formation. In addition, HBJ127 can enhance expression of the mannose receptor on the surface of monocytes derived from healthy humans. HBJ127 shows positive staining of murine L929 cells transiently expressing human CD98 heavy chain in IF studies. The antibody can also be used to detect CD98 heavy chain by ELISA.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD98 Antibody (HBJ127) - Azide and BSA Free

• 4F2
• 4F2hc
• 4T2HC
• antigen identified by monoclonal antibodies 4F2, TRA1.10, TROP4, and T43,4F2HC
• CD98 antigen
• CD98 heavy chain
• CD98
• CD98HC
• CH98hc
• FRP-1
• heavy chain
• Lymphocyte activation antigen 4F2 large subunit
• MDU1antigen defined by monoclonal 4F2, heavy chain
• monoclonal 44D7
• NACAE4F2 cell-surface antigen heavy chain
• SLC3A2
• solute carrier family 3 (activators of dibasic and neutral amino acidtransport), member 2,4F2 heavy chain antigen

Background

The CD98 gene is a member of the solute carrier family and encodes a cell surface, transmembrane protein with an alpha amylase domain. The protein exists as the heavy chain of a heterodimer, covalently bound through di-sulfide bonds to one of several possible

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC3A2