CD90.2 Antibody (30-H12)

Images

 
Flow Cytometry: CD90.2 Antibody (30-H12) [NBP1-28036] - Analysis using the PE conjugate of NBP1-28036. Double staining of BALB/c splenocytes with rat anti-mouse CD90.2-FITC and rat anti-mouse CD19- R-PE.
Flow Cytometry: CD90.2 Antibody (30-H12) [NBP1-28036] - Analysis using the Allophycocyanin conjugate of NBP1-28036. Multiple staining of BALB/c splenocytes.
Flow Cytometry: CD90.2 Antibody (30-H12) [NBP1-28036] - Analysis using the FITC conjugate of NBP1-28036. Double staining of 0.3 ug/10^6 BALB/c splenocytes with rat anti-mouse CD90.2-FITC and rat anti-mouse CD19-R-PE.
Flow Cytometry: CD90.2 Antibody (30-H12) [NBP1-28036] - Analysis using the FITC conjugate of NBP1-28036. Staining of 1 ug on Balb/c Splenocytes.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, IHC-Fr, In vitro, IP, ICC
Clone
30-H12
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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Catalog# & Conjugate Size Price

CD90.2 Antibody (30-H12) Summary

Immunogen
Mouse thymus or spleen
Specificity
Mouse CD90.2/Thy-1.2 alloantigen, Mr 18 kDa
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Rat
Gene
THY1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry <= 1 ug/10^6 cells
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunoprecipitation 1:10-1:500
  • In vitro assay
  • Immunocytochemistry
Publications
Read Publications using
NBP1-28036 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1M BBS (pH 8.2)
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD90.2 Antibody (30-H12)

  • CD90 antigen
  • CD90
  • CDw90
  • FLJ33325
  • Thy-1 antigen
  • Thy-1 cell surface antigen
  • thy-1 membrane glycoprotein
  • Thy-1 T-cell antigen

Background

CD90/Thy-1 is a GPI-anchored molecule and one of the smallest members of the immunoglobulin superfamily, consisting of a single V-set domain. (1-3) The Thy-1.2 alloantigen is expressed on all thymocytes, peripheral T lymphocytes, and some intraepithelial T cells of most mouse strains. It is also expressed in the brain and at varying levels on other non-lymphoid tissues. The 30-H12 monoclonal antibody does not cross react with mouse and rat strains bearing the Thy-1.1 alloantigen (e.g., AKR/J, PL). (2-5)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD90.2 Antibody (NBP1-28036)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: FLOW.


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FLOW
(1)
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Mouse
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FAQs for CD90.2 Antibody (NBP1-28036). (Showing 1 - 1 of 1 FAQs).

  1. I am looking for an anti-mouse Thy1.2 to use in immunofluorescence. However, my samples are formalin-fixed paraffin embedded (FFPE). Have any of your antibodies been tested for reactivity against fixed epitopes that would be worth trying on my samples?
    • We have not tested these antibodies in IHC-P yet, however, I do not see any reason why these products should not work in this application! Because this product worked well in IHC-Fr and FLOW applications wherein the antibodies bind the antigen in relatively native state/sub-cellular form, we strongly believe that it should work in IHC-P also! However, you will certainly need to play around the antigen retrieval timings (depending upon for how long your tissues were kept for fixation in formalin) to get your staining procedure optimized.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NBP1-28040
FITC Labeled NBP1-28037
PE Labeled NBP1-28039

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CD90.2 NBP1-28036

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Bioinformatics

Gene Symbol THY1