Flow Cytometry: CD90.2 Antibody (30-H12) [NBP1-28036] - Analysis using the FITC conjugate of NBP1-28036. Staining of 1 ug on Balb/c Splenocytes.
Flow Cytometry: CD90.2 Antibody (30-H12) [NBP1-28036] - Analysis using the Allophycocyanin conjugate of NBP1-28036. Multiple staining of BALB/c splenocytes.
Flow Cytometry: CD90.2 Antibody (30-H12) [NBP1-28036] - Analysis using the FITC conjugate of NBP1-28036. Double staining of 0.3 ug/10^6 BALB/c splenocytes with rat anti-mouse CD90.2-FITC and rat anti-mouse CD19-R-PE.
CD90/Thy-1 is a GPI-anchored molecule and one of the smallest members of the immunoglobulin superfamily, consisting of a single V-set domain. (1-3) The Thy-1.2 alloantigen is expressed on all thymocytes, peripheral T lymphocytes, and some intraepithelial T cells of most mouse strains. It is also expressed in the brain and at varying levels on other non-lymphoid tissues. The 30-H12 monoclonal antibody does not cross react with mouse and rat strains bearing the Thy-1.1 alloantigen (e.g., AKR/J, PL). (2-5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for CD90.2 Antibody (NBP1-28036). (Showing 1 - 1 of 1 FAQs).
I am looking for an anti-mouse Thy1.2 to use in immunofluorescence. However, my samples are formalin-fixed paraffin embedded (FFPE). Have any of your antibodies been tested for reactivity against fixed epitopes that would be worth trying on my samples?
We have not tested these antibodies in IHC-P yet, however, I do not see any reason why these products should not work in this application! Because this product worked well in IHC-Fr and FLOW applications wherein the antibodies bind the antigen in relatively native state/sub-cellular form, we strongly believe that it should work in IHC-P also! However, you will certainly need to play around the antigen retrieval timings (depending upon for how long your tissues were kept for fixation in formalin) to get your staining procedure optimized.
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