CD90.2 Antibody (30-H12) [FITC]

Flow Cytometry: CD90.2 Antibody (30-H12) [FITC] [NBP1-28037] - 0.3 ug/10^6 BALB/c splenocytes were double-stained with rat anti-mouse CD90.2-FITC and rat anti-mouse CD19-R-PE. Small lymphocytes were then gated and analyzed on a flow cytometer

Flow Cytometry: CD90.2 Antibody (30-H12) [FITC] [NBP1-28037] - 1ug on Balb/c Splenocytes

• Reactivity: Mu
• Applications: Flow, Func, IHC, ICC/IF
• Clone: 30-H12
• Clonality: Monoclonal
• Host: Rat
• Conjugate: FITC
• Concentration: 0.1 mg/ml

CD90.2 Antibody (30-H12) [FITC] Summary

• Immunogen: Mouse thymus or spleen
• Specificity: Mouse CD90.2/Thy-1.2 alloantigen, Mr 18 kDa
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: THY1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1 ug/10^6 cells
  • Immunocytochemistry
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • In vitro assay

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for CD90.2 Antibody (30-H12) [FITC]

• CD90 antigen
• CD90
• CDw90
• FLJ33325
• Thy-1 antigen
• Thy-1 cell surface antigen
• thy-1 membrane glycoprotein
• Thy-1 T-cell antigen

Background

CD90/Thy-1 is a GPI-anchored molecule and one of the smallest members of the immunoglobulin superfamily, consisting of a single V-set domain. (1-3) The Thy-1.2 alloantigen is expressed on all thymocytes, peripheral T lymphocytes, and some intraepithelial T cells of most mouse strains. It is also expressed in the brain and at varying levels on other non-lymphoid tissues. The 30-H12 monoclonal antibody does not cross react with mouse and rat strains bearing the Thy-1.1 alloantigen (e.g., AKR/J, PL). (2-5)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for CD90.2 Antibody (NBP1-28037) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: IL1-R KO Mouse and Mouse spleen.

reviewed by: James Xiao Flow IL1-R KO Mouse and Mouse spleen 01/03/2018

Summary

• Application: Flow Cytometry
• Sample Tested: Mouse spleen cells
• Species: IL1-R KO Mouse and Mouse spleen
• Lot: A5910-Q476Z

Comments

• Comments: The single cell suspension was isolated from a spleen of an IL1R-KO (knockout) mouse and stained with FITC-Conjugated CD90.2 Antibody for 30 minutes. Cells were analyzed with flow cytometer.

Bioinformatics

• Gene Symbol: THY1