CD9 Antibody (5G6)

Images

 
Western Blot: CD9 Antibody (5G6) [NBP2-22187] - Western blot analysis using CD9 mAb against HEK293 (1) and CD9(AA: 37-228)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: CD9 Antibody (5G6) [NBP2-22187] - Immunohistochemical analysis of paraffin-embedded kidney cancer tissues using CD9 mouse mAb with DAB staining.
Flow Cytometry: CD9 Antibody (5G6) [NBP2-22187] - Flow cytometric analysis of Jurkat cells using CD9 mouse mAb (green) and negative control (red).
Immunohistochemistry-Paraffin: CD9 Antibody (5G6) [NBP2-22187] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CD9 mouse mAb with DAB staining.
ELISA: CD9 Antibody (5G6) [NBP2-22187] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)
Immunofluorescence: CD9 Antibody (5G6) [NBP2-22187] - Human endometrial stromal cells stained with anti-CD9 antibody and detected with anti-mouse IgG AlexaFluor 488 secondary antibody (Green). Image from verified ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P, IF
Clone
5G6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CD9 Antibody (5G6) Summary

Immunogen
Synthesized peptide of human CD9.
Localization
Cytoplasm, Membrane
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD9
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunofluorescence
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-22187 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for CD9 Antibody (5G6)

  • BA2
  • BA-2/p24 antigen
  • BTCC-1
  • CD9 antigen
  • CD9 molecule
  • CD9
  • Cell growth-inhibiting gene 2 protein
  • DRAP-27
  • Leukocyte antigen MIC3
  • MIC3
  • motility related protein-1,5H9 antigen
  • Motility-related protein
  • MRP-1FLJ99568
  • p24
  • tetraspanin-29
  • TSPAN29
  • TSPAN-29
  • TSPAN29MIC3CD9 antigen (p24)

Background

This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD9 Antibody (NBP2-22187) (0)

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Review for CD9 Antibody (NBP2-22187) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IF.

Reviews using NBP2-22187:
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IF
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Images Ratings Applications Species Date Details
Immunofluorescence CD9 NBP2-22187
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reviewed by:
Gareth Healey
IF Human 01/18/2016
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Summary

ApplicationImmunofluorescence
Sample TestedHuman endometrial stromal cells
SpeciesHuman

Blocking

Blocking Details3% BSA in PBS, 1 hour at room temperature

Primary Anitbody

Dilution Ratio1 in 200 in 3% BSA (in PBS), 2 hours at room temp

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG, AlexFluor 488
Secondary Manufacturer Cat#A11001
Secondary Concentration1 in 1000 dilution in 3% BSA

Details

Detection NotesConfocal microscopy, Zeiss LSM 710, Objective: 40x/1.40 Oil DIC M27, neg conts = unstained, primary only, secondary only
Fixation Details4% PFA for 20 min at 4C

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for CD9 Antibody (NBP2-22187) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Gareth Healey
01/18/2016
Application: IF
Species: Human

Bioinformatics

Gene Symbol CD9
Entrez