CD7 Antibody

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Western blot shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and human peripheral blood lymphocytes (PBL). PVDF membrane was probed with 0.5 µg/mL ...read more
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Sheep Anti-Human CD7 Antigen Affinity-purified ...read more
CD7 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Sheep Anti-Human CD7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7579) at 15 µg/mL for 3 hours at room ...read more
CD7 was detected in immersion fixed paraffin-embedded sections of human thymus using Sheep Anti-Human CD7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7579) at 3 µg/mL overnight at 4 °C. Before ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

CD7 Antibody Summary

Immunogen
Kidney embryonic cell line HEK293-derived recombinant human CD7
Ala26-Pro180
Accession # P09564
Specificity
Detects human CD7 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CD7 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
CD7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publication using
AF7579 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD7 Antibody

  • CD7 antigen (p41)
  • CD7 antigen
  • CD7 molecule
  • CD7
  • GP40T-cell surface antigen Leu-9
  • LEU-9
  • T-cell antigen CD7
  • T-cell leukemia antigen
  • Tp40
  • TP41
  • TP41p41 protein

Background

CD7 (Cluster of Differentiation Antigen 7; also Leu-9, TP41 and GP40) is a 40-44 kDa member of the Ig-superfamily of proteins. It shows restricted expression, being found on fetal thymocytes, CD34+ myeloid and lymphoid progenitor cells, memory CLA- CD45RA+ T cells, and CD56+ IFN-gamma secreting NK cells. CD7 binds to both SECTM1/K12 and galectin-1, and when bound to the latter, initiates complex formation with CD43 in cis. Activation of CD7 may result in either cell proliferation or apoptosis, suggesting a context-dependent signaling mechanism. Mature human CD7 is a 215 amino acid (aa) type I transmembrane glycoprotein. It contains a 155 aa extracellular region (aa 26-180) that shows one V-type Ig-like domain (aa 26-130), and a 39 aa C-terminal cytoplasmic domain. There is one potential alternative splice variant that contains a 79 aa substitution for aa 133-240. Over aa 26-180, human CD7 shares only 43% aa sequence identity with mouse CD7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD7 Antibody (AF7579)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


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Bioinformatics

Gene Symbol CD7
Uniprot