TdT Antibody

Images

 
Western Blot: TdT Antibody [NBP2-31368] - WB detection of TDT protein in (A) lysate of MOLT4 human leukemia cell line and (B) partial recombinant protein by using TDT antibody. Primary antibody concentration used: 1 ...read more
Immunocytochemistry/ Immunofluorescence: TdT Antibody [NBP2-31368] - TdT antibody was tested in A431 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). ...read more
Immunohistochemistry-Paraffin: TdT Antibody [NBP2-31368] - IHC-P analysis of TdT protein in a section of human hepatocellular carcinoma (HCC) using TdT antibody at a concentration of 10 ug/ml. The HCC cells depicted ...read more
Immunohistochemistry-Paraffin: TdT Antibody [NBP2-31368] - IHC-P analysis of TdT protein in a section of human lung using TdT antibody at a concentration of 10 ug/ml. The alveolar cells of the lungs showed strong ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

TdT Antibody Summary

Immunogen
Partial recombinant protein made to an internal sequence of human TdT (between residues 50-300) [UniProt# P04053]
Localization
Nucleus (high), Cytoplasm (very low)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DNTT
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1.0 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
Application Notes
TdT is a 509 amino acids long protein with predicted molecular weight of 58.5 kDa, and it localizes mainly to the nucleus with low amounts in cytoplasm of the cells. In our Western blot validation with MOLT4 lysate, this TdT antibody detected a single specific band at ~66 kDa position. In our immunohistochemistry validation on formalin fixed paraffin embedded tissue sections, this antibody developed a strong nuclear/ weak cytoplasmic staining in normal lung and hepatocellular carcinoma of human. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
58.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human. Immunogen's sequence similarity with other species: Rat (77%) Mouse (74%), Bovine (78%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for TdT Antibody

  • deoxynucleotidyltransferase, terminal
  • DNTT
  • EC 2.7.7
  • EC 2.7.7.31
  • nucleosidetriphosphate:DNA deoxynucleotidylexotransferase
  • TdT
  • TDTDNA nucleotidylexotransferase
  • Terminal addition enzyme
  • Terminal deoxynucleotidyltransferase
  • terminal deoxyribonucleotidyltransferase
  • Terminal transferase

Background

TdT is a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxly terminus of oligonucleotide primers. It is expressed in a limited population of normal and malignant pre-B and pre-T lymphocytes during early differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on TdT.

TdT (Terminal Deoxynucleotidyl Transferase)
The enzyme family of DNA polymerases plays a fundamental role in the replication, repair, and recombination of nucleic acid. Its members include DNA Polymerase b (Pol b), DNA Polymerase g (Pol g), and DNA Polymerase m (Pol m). TdT is a very unique and...  Read full blog post.

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Bioinformatics

Gene Symbol DNTT
Entrez
Uniprot