CD7 Antibody [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB, Flow, IHC, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 405

CD7 Antibody [Alexa Fluor® 405] Summary

• Immunogen: Kidney embryonic cell line HEK293-derived recombinant human CD7
  Ala26-Pro180
  Accession # P09564
• Specificity: Detects human CD7 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CD7 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD7 Antibody [Alexa Fluor® 405]

• CD7 antigen (p41)
• CD7 antigen
• CD7 molecule
• CD7
• GP40T-cell surface antigen Leu-9
• LEU-9
• T-cell antigen CD7
• T-cell leukemia antigen
• Tp40
• TP41
• TP41p41 protein

Background

CD7 (Cluster of Differentiation Antigen 7; also Leu-9, TP41 and GP40) is a 40-44 kDa member of the Ig-superfamily of proteins. It shows restricted expression, being found on fetal thymocytes, CD34⁺ myeloid and lymphoid progenitor cells, memory CLA- CD45RA⁺ T cells, and CD56⁺ IFN-gamma secreting NK cells. CD7 binds to both SECTM1/K12 and galectin-1, and when bound to the latter, initiates complex formation with CD43 in cis. Activation of CD7 may result in either cell proliferation or apoptosis, suggesting a context-dependent signaling mechanism. Mature human CD7 is a 215 amino acid (aa) type I transmembrane glycoprotein. It contains a 155 aa extracellular region (aa 26-180) that shows one V-type Ig-like domain (aa 26-130), and a 39 aa C-terminal cytoplasmic domain. There is one potential alternative splice variant that contains a 79 aa substitution for aa 133-240. Over aa 26-180, human CD7 shares only 43% aa sequence identity with mouse CD7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.