CD7 Antibody (3A1E) - Chimeric - Azide and BSA Free

Western Blot: CD7 Antibody (3A1E) [NBP2-81050] - Western Blot using anti-CD7 antibody (3A1E) [NBP2-81050]. Jurkat cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 3A1E [NBP2-81050] at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified CD7 is 25.4kDa, but this protein is glycosylated at several residues. NBP2-81050 successfully detected CD7 in Jurkat cell lysate.

Immunocytochemistry/Immunofluorescence: CD7 Antibody (3A1E) [NBP2-81050] - Immunofluorescence staining of fixed Jurkat cells with anti-CD7 antibody (3A1E) [NBP2-81050]. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 3A1E [NBP2-81050] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81050, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu
• Applications: WB, Flow, Func, ICC/IF
• Clone: 3A1E
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CD7 Antibody (3A1E) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit CD7 Antibody (3A1E) - Chimeric - Azide and BSA Free (NBP2-81050) is a recombinant monoclonal antibody validated for use in WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody. Clone 3A1E was used by HLDA to establish CD designation.
• Immunogen: CD7
• Specificity: Recognises human CD7.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CD7
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Functional
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD7 Antibody (3A1E) - Chimeric - Azide and BSA Free

• CD7 antigen (p41)
• CD7 antigen
• CD7 molecule
• CD7
• GP40T-cell surface antigen Leu-9
• LEU-9
• T-cell antigen CD7
• T-cell leukemia antigen
• Tp40
• TP41
• TP41p41 protein

Background

CD7, also known as gp40, is a 40 kD type I transmembrane glycoprotein member of the immunoglobulin superfamily. It is found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL). CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD7