CD45RO Antibody (UCHL-1) - Azide and BSA Free

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Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P, IP, CyTOF-ready
Clone
UCHL-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

CD45RO Antibody (UCHL-1) - Azide and BSA Free Summary

Immunogen
Interleukin-2-dependent human T lymphocytes were used as the immunogen for the CD45RO antibody.
Localization
Cell surface
Marker
T-Cell Marker
Specificity
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO) (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PTPRC
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1.0ug/ml
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • Immunoprecipitation 0.5-1ug/500ug protein lysate
  • CyTOF-ready
Application Notes
Although not required, staining of formalin/paraffin tissues is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Immunohistochemistry-Frozen application reported in literature.
Theoretical MW
182 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Chimpanzee, Common Marmoset. Others not known.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10mM PBS - BSA free
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for CD45RO Antibody (UCHL-1) - Azide and BSA Free

  • B220
  • CD45 antigen
  • CD45R
  • CD45RO
  • EC 3.1.3.48
  • L-CA
  • LY5
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • T200 glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD45RO Antibody (NBP2-33104). (Showing 1 - 1 of 1 FAQ).

  1. I cannot find any anti-mouse CD45RO for FACS. Have any of your anti-Human CD45RO been tested on mouse T cells?
    • Unfortunately, none of our CD45RO antibodies for Flow Cytometry have been validated on mouse. If you would be interested in testing the antibody on mouse, we would recommend Our Innovators Reward Program. Under the terms and conditions of this program we would be happy to provide you with a credit of equal or lesser value, in exchange for your online review including an image of either positive or negative staining for testing in a new species and/or application. Please contact us at innovators@novusbio.com with any questions regarding this program.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-33104AF405
Alexa Fluor 488 Labeled NBP2-33104AF488
Alexa Fluor 647 Labeled NBP2-33104AF647
Alexa Fluor 700 Labeled NBP2-33104AF700
Allophycocyanin Labeled NBP2-33104APC
Biotin Labeled NBP2-33104B
DyLight 350 Labeled NBP2-33104UV
DyLight 405 Labeled NBP2-33104V
DyLight 488 Labeled NBP2-33104G
DyLight 550 Labeled NBP2-33104R
DyLight 650 Labeled NBP2-33104C
DyLight 680 Labeled NBP2-33104FR
DyLight 755 Labeled NBP2-33104IR
HRP Labeled NBP2-33104H
PE Labeled NBP2-33104PE
PerCP Labeled NBP2-33104PCP

Additional CD45RO Products

CD45RO NBP2-33104

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Bioinformatics

Gene Symbol PTPRC
Entrez