CD45RO Antibody (UCHL-1) [PE/Atto594]

• Reactivity: Hu, Pm, Pm
• Applications: Flow, ICC/IF, IHC, CyTOF-ready, Dual ISH-IHC
• Clone: UCHL-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE/Atto594

CD45RO Antibody (UCHL-1) [PE/Atto594] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Cultured human T-cells from an IL-2-dependent T-cell line (CA1)
• Localization: Cell surface
• Marker: T-Cell Marker
• Specificity: Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO) (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPRC
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Dual RNAscope ISH-IHC
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Common Marmoset

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for CD45RO Antibody (UCHL-1) [PE/Atto594]

• B220
• CD45 antigen
• CD45R
• CD45RO
• EC 3.1.3.48
• L-CA
• LY5
• protein tyrosine phosphatase, receptor type, C
• receptor-type tyrosine-protein phosphatase C
• T200 glycoprotein
• T200 leukocyte common antigen
• T200receptor type, c polypeptide

Background

CD45RO is a single chain transmembraneous glycoprotein which represents the low molecular weight isoform of the Leucocyte Common Antigen (LCA). It is expressed on most thymocytes, about 45% of peripheral blood T cells, virtually all T cells in skin reactive infiltrates, and the majority of T cell malignancies. It is also found on a subset of B cells and on exceptional B cell lymphomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for CD45RO Antibody (NBP2-33104PEATT594). (Showing 1 - 1 of 1 FAQ).

1. I cannot find any anti-mouse CD45RO for FACS. Have any of your anti-Human CD45RO been tested on mouse T cells?
   • Unfortunately, none of our CD45RO antibodies for Flow Cytometry have been validated on mouse. If you would be interested in testing the antibody on mouse, we would recommend Our Innovators Reward Program. Under the terms and conditions of this program we would be happy to provide you with a credit of equal or lesser value, in exchange for your online review including an image of either positive or negative staining for testing in a new species and/or application. Please contact us at innovators@novusbio.com with any questions regarding this program.

Bioinformatics

• Gene Symbol: PTPRC