CD45RB Antibody (SPM569) - IHC-Prediluted

Immunohistochemistry-Paraffin: CD45RB Antibody (SPM569) - IHC-Prediluted [NBP2-44856] - Formalin-fixed, paraffin-embedded human tonsil (20X) stained with CD45RB MAb (SPM569).

Immunohistochemistry-Paraffin: CD45RB Antibody (SPM569) - IHC-Prediluted [NBP2-44856] - Formalin-fixed, paraffin-embedded human lymphoma stained with CD45RB Monoclonal Antibody (SPM569)

Western Blot Analysis of human spleen tissue lysates using CD45RB Antibody (SPM569) - IHC-Prediluted.

• Reactivity: Hu
• Applications: IHC
• Clone: SPM569
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: IHC-Prediluted

CD45RB Antibody (SPM569) - IHC-Prediluted Summary

• Description: The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
• Immunogen: Human peripheral blood lymphocytes maintained in T cell growth factor
• Localization: Cell surface and cytoplasmic
• Marker: Leucocyte Marker
• Specificity: CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPRC
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for CD45RB Antibody (SPM569) - IHC-Prediluted

• B220
• CD45 antigen
• CD45R
• CD45RB
• EC 3.1.3.48
• L-CA
• LY5
• protein tyrosine phosphatase, receptor type, C
• receptor-type tyrosine-protein phosphatase C
• T200 glycoprotein
• T200 leukocyte common antigen
• T200receptor type, c polypeptide

Background

CD45RB is an alternatively spliced form of CD45, a cell surface glycoprotein. It is expressed on peripheral B cells, T cytotoxic cells and CD4+ T cells. It has a role in cell signalling regulation through the activation of the Src family of tyrosine kinases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPRC