CD45RB Antibody (SPM569) [DyLight 755]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP
Clone
SPM569
Clonality
Monoclonal
Host
Mouse

CD45RB Antibody (SPM569) [DyLight 755] Summary

Immunogen
Human peripheral blood lymphocytes maintained in T cell growth factor
Localization
Cell surface and cytoplasmic
Marker
B-Cell Marker
Specificity
CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PTPRC
Purity
Protein A purified
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Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
DyLight 755
Format
DyLight 755 (A=754, E=776)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
Immunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Alternate Names for CD45RB Antibody (SPM569) [DyLight 755]

  • B220
  • CD45 antigen
  • CD45R
  • EC 3.1.3.48
  • L-CA
  • LY5
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • T200 glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD45RB Antibody (NBP2-34803IR) (0)

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Secondary Antibodies

 

Isotype Controls

Additional CD45RB Antibody (SPM569) [DyLight 755] Products

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Bioinformatics Tool for CD45RB Antibody (NBP2-34803IR)

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Diseases for CD45RB Antibody (NBP2-34803IR)

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PTMs for CD45RB Antibody (NBP2-34803IR)

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol PTPRC

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