CD45 Antibody (F10-89-4)


Flow Cytometry: CD45 Antibody (F10-89-4) [NBP1-28509] - Analysis using the PE conjugate of NBP1-28509. Multiple staining of human peripheral blood lymphocytes.
Flow Cytometry: CD45 Antibody (F10-89-4) [NBP1-28509] - Analysis using the Biotin conjugate of NBP1-28509. Multiple staining of peripheral blood lymphocytes.
Flow Cytometry: CD45 Antibody (F10-89-4) [NBP1-28509] - Analysis using the Cy5 conjugate of NBP1-28509. Multiple staining of peripheral blood lymphocytes.
Flow Cytometry: CD45 Antibody (F10-89-4) [NBP1-28509] - Analysis using the FITC conjugate of NBP1-28509. Multiple staining of peripheral blood lymphocytes.

Product Details

Reactivity Hu, EqSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr, IP, ICC
0.1 mg/ml

Order Details

Available Conjugates
Catalog# & Conjugate Size Price

CD45 Antibody (F10-89-4) Summary

The immunogen is a peptide made to the amino acid region CD45
Human CD45
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
BBS (pH 8.2)
No Preservative
0.1 mg/ml
Protein A or G purified


  • Western Blot 1:100 - 1:2000
  • Flow Cytometry <= 1 ug/10^6 cells
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Immunocytochemistry
Read Publications using
NBP1-28509 in the following applications:

Reactivity Notes


Alternate Names for CD45 Antibody (F10-89-4)

  • B220
  • CD45 antigen
  • CD45
  • CD45R
  • EC
  • LCA
  • L-CA
  • LY5
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • T200 glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide


CD45, also known as the leukocyte common antigen (LCA), exists as four different isoforms (180, 190, 205, 220 kDa) that are generated by alternative splicing of three exons. LCA is found on all cells of hematopoietic origin, except erythrocytes and platelets. CD45 expression is required for T cell and B cell antigen receptor-mediated activation. (1-4)


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD45 Antibody (NBP1-28509)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: FLOW.

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Showing Publications 1 - 2 of 2.
Publications using NBP1-28509 Applications Species
Wu Y, Deighan CJ, Miller BL et al. Isolation and analysis of rare cells in the blood of cancer patients using a negative depletion methodology. Methods. 2013 Sep 20 [PMID:24056212] (FLOW, Human) FLOW Human
Duell BL, Carey AJ, Tan CK et al. Innate Transcriptional Networks Activated in Bladder in Response to Uropathogenic Escherichia coli Drive Diverse Biological Pathways and Rapid Synthesis of IL-10 for Defense against Bacterial Urinary Tract

Reviews for CD45 Antibody (NBP1-28509) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CD45 Antibody (NBP1-28509). (Showing 1 - 2 of 2 FAQs).

  1. We are interested in CD45 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • I would recommend one of these CD45 antibodies to you, as these have all been validated for use with dog/canine samples.
  2. We would like to order a CD45 antibody to stain immune cells that were isolated from a ligated sciatic nerve of mice with double immunofluorescence (using PFA-fixed tissue on slides). Which of the following rat monoclonal CD45 antibodies would you recommend: 30-F11, IBL-3/16 or 5C16?
    • I would recommend clone 30-F11. As clone IBL-3/16 has not yet been validated for IHC-P application, we would not be able to guarantee its workability on PFA-fixed tissue. Although both other clones i.e. 30-F11 and 5C16, would be good for your samples, CD45 30-F11 # NB100-77417 is a well known clone that offers more flexibility around protocol because of availability of its conjugated forms. It would be advantageous to use a conjugated primary as you are planning for double-immunostaining procedure.

Secondary Antibodies


Isotype Controls

Other Available Formats

Biotin Labeled NBP1-28511
Cy5 Labeled NBP1-28513
FITC Labeled NBP1-28510
PE Labeled NBP1-28512

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Blogs on CD45.

CD45 - Much more than just a housekeeping protein
CD45, also known as T200 or the Leukocyte Common Antigen (LCA), is encoded by the Protein Tyrosine Phosphatase Receptor type C (PTPRC) gene. The protein is expressed exclusively on cells of the haematopoietic system, and is one of the most abundant le...  Read full blog post.

CD45 Isoforms: Hematopoietic Differentiation, Cancer and Alzheimer's
CD45, also known as protein tyrosine phosphatase, receptor type, C (PTPRC), was originally known as common leukocyte antigen and is a signal transducer involved in many physiological processes such as growth and differentiation, cancer transformation,...  Read full blog post.

Vimentin Antibodies in Rheumatoid Arthritis & Cataracts Research
Vimentin is a 57kDa type III intermediate filament (IF) protein that is the major cytoskeletal component of mesenchymal cells and the first to be expressed during cell differentiation. It plays a significant role in supporting and anchoring the positi...  Read full blog post.

Antibodies In The Differential Diagnosis Of Undifferentiated Tumors
Immunohistochemical testing using antibody panels has a valuable role in cancer diagnosis. Some tumors, especially more malignant ones, tend to lose the appearance of the tissue of origin. However, knowing the tissue of origin assists the physician in...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol PTPRC

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