Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
A 0.2 um filtered solution in PBS
Protein G purified
Reconstitute with 0.5 ml sterilized PBS to a final concentration of 0.4 mg/ml.
Alternate Names for CD45 Antibody (5C16)
protein tyrosine phosphatase, receptor type, C
receptor-type tyrosine-protein phosphatase C
T200 leukocyte common antigen
T200receptor type, c polypeptide
CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for CD45 Antibody (NB110-93609). (Showing 1 - 2 of 2 FAQs).
We are interested in CD45 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
I would recommend one of these CD45 antibodies to you, as these have all been validated for use with dog/canine samples.
We would like to order a CD45 antibody to stain immune cells that were isolated from a ligated sciatic nerve of mice with double immunofluorescence (using PFA-fixed tissue on slides). Which of the following rat monoclonal CD45 antibodies would you recommend: 30-F11, IBL-3/16 or 5C16?
I would recommend clone 30-F11. As clone IBL-3/16 has not yet been validated for IHC-P application, we would not be able to guarantee its workability on PFA-fixed tissue. Although both other clones i.e. 30-F11 and 5C16, would be good for your samples, CD45 30-F11 # NB100-77417 is a well known clone that offers more flexibility around protocol because of availability of its conjugated forms. It would be advantageous to use a conjugated primary as you are planning for double-immunostaining procedure.
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