CD45 Antibody (5C16)


Immunohistochemistry: CD45 Antibody (5C16) [NB110-93609] - CD45 immunohistochemistry staining of paraffin sections of mouse kidney and liver tissues from LPS exposed animals.

Product Details

Reactivity MuSpecies Glossary
Applications ICC/IF, IHC, IHC-P, IP
LYOPH mg/ml

Order Details

CD45 Antibody (5C16) Summary

Cell lysates of mouse leukocytes
This was selected for its ability to detect mouse CD45 positive leukocytes in IHC.
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
A 0.2 um filtered solution in PBS
No Preservative
LYOPH mg/ml
Protein G purified
Reconstitution Instructions
Reconstitute with 0.5 ml sterilized PBS to a final concentration of 0.4 mg/ml.


  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100-400
  • Immunohistochemistry-Paraffin 1:100-400
  • Immunoprecipitation 1:10-1:500
Read Publications using
NB110-93609 in the following applications:

Alternate Names for CD45 Antibody (5C16)

  • B220
  • CD45 antigen
  • CD45
  • CD45R
  • EC
  • LCA
  • L-CA
  • LY5
  • protein tyrosine phosphatase, receptor type, C
  • receptor-type tyrosine-protein phosphatase C
  • T200 glycoprotein
  • T200 leukocyte common antigen
  • T200receptor type, c polypeptide


CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Species: Hu
Applications: Flow, Func, In vitro, In vivo, Flow-CS
Species: Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, InhibTFunc
Species: Hu, Mu
Applications: WB, Flow
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rb(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Species: Hu
Applications: Flow
Species: Hu
Applications: WB, IHC, IHC-P
Species: Mu
Applications: WB, Flow, IHC
Species: Mu
Applications: ICC/IF, IHC, IHC-P, IP

Publications for CD45 Antibody (NB110-93609)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: ICC/IF, IHC, IHC-P.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 3 of 3.
Publications using NB110-93609 Applications Species
Errede M, Girolamo F, Ferrara G et al. Blood-Brain Barrier Alterations in the Cerebral Cortex in Experimental Autoimmune Encephalomyelitis J Neuropathol Exp Neurol 2012 Oct [PMID:23001217] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Girolamo F, Ferrara G, Strippoli M et al. Cerebral cortex demyelination and oligodendrocyte precursor response to experimental autoimmune encephalomyelitis Neurobiol Dis 2011 Sep [PMID:21679768] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Buchel G, Schulte JH, Harrison L, Batzke K et al. Immune response modulation by Galectin-1 in a transgenic model of neuroblastoma OncoImmunology Feb 18 2016 12:00AM (IHC-P, Mouse) IHC-P Mouse

Reviews for CD45 Antibody (NB110-93609) (0)

There are no reviews for CD45 Antibody (NB110-93609). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for CD45 Antibody (NB110-93609). (Showing 1 - 2 of 2 FAQs).

  1. We are interested in CD45 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • I would recommend one of these CD45 antibodies to you, as these have all been validated for use with dog/canine samples.
  2. We would like to order a CD45 antibody to stain immune cells that were isolated from a ligated sciatic nerve of mice with double immunofluorescence (using PFA-fixed tissue on slides). Which of the following rat monoclonal CD45 antibodies would you recommend: 30-F11, IBL-3/16 or 5C16?
    • I would recommend clone 30-F11. As clone IBL-3/16 has not yet been validated for IHC-P application, we would not be able to guarantee its workability on PFA-fixed tissue. Although both other clones i.e. 30-F11 and 5C16, would be good for your samples, CD45 30-F11 # NB100-77417 is a well known clone that offers more flexibility around protocol because of availability of its conjugated forms. It would be advantageous to use a conjugated primary as you are planning for double-immunostaining procedure.

Secondary Antibodies


Isotype Controls

Additional CD45 Antibody (5C16) Products

Related Products by Gene

Bioinformatics Tool for CD45 Antibody (NB110-93609)

Discover related pathways, diseases and genes to CD45 Antibody (NB110-93609). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CD45 Antibody (NB110-93609)

Discover more about diseases related to CD45 Antibody (NB110-93609).

Pathways for CD45 Antibody (NB110-93609)

View related products by pathway.

PTMs for CD45 Antibody (NB110-93609)

Learn more about PTMs related to CD45 Antibody (NB110-93609).

Research Areas for CD45 Antibody (NB110-93609)

Find related products by research area.

Blogs on CD45.

CD45 - Much more than just a housekeeping protein
CD45, also known as T200 or the Leukocyte Common Antigen (LCA), is encoded by the Protein Tyrosine Phosphatase Receptor type C (PTPRC) gene. The protein is expressed exclusively on cells of the haematopoietic system, and is one of the most abundant le...  Read full blog post.

CD45 Isoforms: Hematopoietic Differentiation, Cancer and Alzheimer's
CD45, also known as protein tyrosine phosphatase, receptor type, C (PTPRC), was originally known as common leukocyte antigen and is a signal transducer involved in many physiological processes such as growth and differentiation, cancer transformation,...  Read full blog post.

Vimentin Antibodies in Rheumatoid Arthritis & Cataracts Research
Vimentin is a 57kDa type III intermediate filament (IF) protein that is the major cytoskeletal component of mesenchymal cells and the first to be expressed during cell differentiation. It plays a significant role in supporting and anchoring the positi...  Read full blog post.

Antibodies In The Differential Diagnosis Of Undifferentiated Tumors
Immunohistochemical testing using antibody panels has a valuable role in cancer diagnosis. Some tumors, especially more malignant ones, tend to lose the appearance of the tissue of origin. However, knowing the tissue of origin assists the physician in...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol PTPRC

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB110-93609 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought