CD44 Antibody (CD44v9/1459) - variant 9

Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 [NBP2-53204] - Formalin-fixed, paraffin-embedded human Tongue Carcinoma stained with CD44 Antibody (CD44v9/1459) - variant 9.

Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 [NBP2-53204] - Formalin-paraffin human Cervical Carcinoma stained with CD44 Monoclonal Antibody (CD44v9/1459)

Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 [NBP2-53204] - Formalin-paraffin human Prostate Carcinoma stained with CD44 Monoclonal Antibody (CD44v9/1459)

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CD44v9/1459
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CD44 Antibody (CD44v9/1459) - variant 9 Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54571)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant fragment corresponding to the v9 domain of human CD44 (Uniprot: P16070)
• Localization: Cell surface
• Marker: Marker of Tumor Metastasis
• Specificity: This antibody recognizes an epitope encoded by exon v9 on the variant portion of human CD44. The CD44 molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. CD44 is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA.The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD44
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot
• Application Notes: Use in WB reported in scientific literature (PMID:34490820)
  Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD44 Antibody (CD44v9/1459) - variant 9

• CD44 antigen
• CD44 molecule (Indian blood group)
• CD44
• CD44R
• CDw44
• cell surface glycoprotein CD44
• chondroitin sulfate proteoglycan 8
• CSPG8
• ECMR-III
• epican
• Extracellular matrix receptor III
• GP90 lymphocyte homing/adhesion receptor
• HCAM
• HCELL
• hematopoietic cell E- and L-selectin ligand
• Heparan sulfate proteoglycan
• Hermes antigen
• homing function and Indian blood group system
• HUTCH-I
• Hyaluronate receptor
• IN
• LHR
• MC56
• MDU2
• MDU2CD44 antigen (homing function and Indian blood group system)
• MDU3
• MDU3CDW44
• MIC4
• MIC4MGC10468
• MUTCH-I
• Pgp1
• PGP-1
• PGP-I
• Phagocytic glycoprotein 1
• Phagocytic glycoprotein I

Background

CD44 represents a family of type I transmembrane glycoproteins expressed on many cell types such as B cells, monocytes, macrophages, and variable subsets of thymocytes and peripheral T cells. CD44 primarily functions as an adhesion molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing, leukocyte activation, tumor metastasis and development. The KM201 monoclonal antibody reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule. KM201 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.1-6

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD44, variant 9 Antibody (NBP2-53204)(2)

Publications using NBP2-53204

• Soon Sang Park, Young-Kyoung Lee, Yong Won Choi, Su Bin Lim, So Hyun Park, Han Ki Kim, Jun Sang Shin, Young Hwa Kim, Dong Hyun Lee, Jang-Hee Kim, Tae Jun Park Cellular senescence is associated with the spatial evolution toward a higher metastatic phenotype in colorectal cancer. Cell reports 2024-04-01 [PMID: 38446659]
• Chang I, Ohn T, Moon D et al. SNU-333 Cells as an Appropriate Cell Line for the Orthotopic Renal Cell Carcinoma Model Technology in cancer research & treatment 2021-09-07 [PMID: 34490820] (WB, Human)

Review for CD44, variant 9 Antibody (NBP2-53204) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Xiuxia Zhou WB Human 04/29/2019

Summary

• Application: Western Blot
• Sample Tested: airway epithelial cells
• Species: Human
• Lot: 960-8P171214

Comments

• Comments: used 1:500 dilution for Western blot

Bioinformatics

• Gene Symbol: CD44
• Uniprot:
  • P16070()
  • P16070()