Western blot shows lysates of C2C12 mouse myoblast cell line, Balb/3T3 mouse embryonic fibroblast cell line, and B16‑F1 mouse melanoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse ...read more
Syndecan-2/CD362 was detected in perfusion fixed frozen sections of mouse spinal cord using Sheep Anti-Mouse Syndecan-2/CD362 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6585) at 1.7 µg/mL overnight at 4 ...read more
NIH-3T3 mouse cell line was stained with Sheep Anti-Mouse Syndecan-2/CD362 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6585, filled histogram) or control antibody (Catalog # 5‑001‑A, open histogram), ...read more
Syndecan-2, previously known as fibroglycan or heparan sulfate proteoglycan, is a member of the syndecan family of type 1 transmembrane proteins capable of carrying heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans. The four vertebrate syndecans show conserved cytoplasmic domains and divergent extracellular portions (except for GAG attachment sites). Among the Syndecans, Syndecan-2 is most similar to Syndecan-4 (1‑3). Mouse Syndecan-2 is synthesized as a 202 amino acid (aa) core protein with an 18 aa signal sequence, a 127 aa extracellular domain (ECD), a 25 aa transmembrane region and a 32 aa cytoplasmic tail (4). The ECD of mouse Syndecan-2 contains three closely-spaced consensus Ser-Gly sequences for the attachment of HS side chains. It shares 76%, 86%, 74% and 72% aa identity with the ECD of human, rat, porcine and bovincoe Syndecan-2, respectively. The cytoplasmic tail has both serine and tyrosine phosphorylation sites. Addition of 20 ‑ 80 disaccharides per side chain adds considerably to the size of the 22 kDa core protein. Non-covalent homodimerization of Syndecan-2, or heterodimerization with Syndecan-4, is dependent on the transmembrane domain (5, 6). Syndecan-2 is expressed in cells of mesenchymal origin, neuronal and epithelial cells, and is the predominant syndecan expressed during embryonic development. Expression is up‑regulated in several cancer cell lines (7). After induction in macrophages by inflammatory mediators, Syndecan-2 selectively binds FGF basic, VEGF and EGF (8). Syndecan-2 expressed on human primary osteoblasts binds GM‑CSF and may function as a co-receptor (9). Activated endothelial cell Syndecan-2 specifically binds IL-8 and may participate in promoting neutrophil extravasation by forming a chemotactic IL-8 gradient (10). Typically, cytokine, chemokine and extracellular matrix protein binding occurs through interaction with HS side chains, but the Syndecan-2 extracellular domain can bind TGF-beta directly via protein-protein interaction (11).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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