CD4 Antibody (GK1.5) [Fluorescein]

Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse CD4 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB554F) or (B) Rat IgG2BFluorescein Isotype Control (Catalog # IC013F). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: GK1.5
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Fluorescein

CD4 Antibody (GK1.5) [Fluorescein] Summary

• Immunogen: Mouse CTL clone V4
• Specificity: Reacts with mouse CD4, an antigen co-receptor on the T cell surface which interacts with MHC II molecules on antigen presenting cells.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: CD4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD4 Antibody (GK1.5) [Fluorescein]

• CD_antigen: CD4
• CD4 antigen (p55)
• CD4 antigen
• CD4 molecule
• CD4 receptor
• CD4
• CD4mut
• T-cell surface antigen T4/Leu-3
• T-cell surface glycoprotein CD4

Background

CD4 is a type I membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed predominantly on thymocytes and a subset of mature T lymphocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD4 Antibody (FAB554F)(7)

Publications using FAB554F

• N Jovicic, I Petrovic, N Pejnovic, B Ljujic, M Miletic Ko, S Pavlovic, I Jeftic, A Djukic, I Srejovic, V Jakovljevi, ML Lukic Transgenic Overexpression of Galectin-3 in Pancreatic beta Cells Attenuates Hyperglycemia in Mice: Synergistic Antidiabetic Effect With Exogenous IL-33 Frontiers in Pharmacology, 2021-11-05;12(0):714683. 2021-11-05 [PMID: 34803672] (Flow Cytometry, Mouse)
• S Mukhopadhy, J Gabre, C Chabasse, JS Bromberg, TM Antalis, R Sarkar Depletion of CD4 and CD8 Positive T Cells Impairs Venous Thrombus Resolution in Mice Int J Mol Sci, 2020-02-28;21(5):. 2020-02-28 [PMID: 32121269] (Flow Cytometry, Mouse)
• Honghua Yuan Early Pregnancy Factor Enhances the Generation and Function of CD4(+)CD25(+) Regulatory T Cells Tohoku J. Exp. Med., 2016-11-01;240(3):215-220. 2016-11-01 [PMID: 27840373] (Flow Cytometry, Mouse)
• Freitas E, Nico D, Alves-Silva M, Morrot A, Clinch K, Evans G, Tyler P, Schramm V, Palatnik-de-Sousa C Immucillins ImmA and ImmH Are Effective and Non-toxic in the Treatment of Experimental Visceral Leishmaniasis. PLoS Negl Trop Dis, 2015-12-23;9(12):e0004297. 2015-12-23 [PMID: 26701750] (Flow Cytometry, Mouse)
• César Muñoz-Fontela, Michael Pazos, Igotz Delgado, William Murk, Sathish Kumar Mungamuri, Sam W. Lee et al. p53 Serves as a Host Antiviral Factor That Enhances Innate and Adaptive Immune Responses to Influenza A Virus The Journal of Immunology 2011-12-15 [PMID: 22105999] (Flow Cytometry, Mouse)
• Notley CA, Brown MA, Wright GP, Ehrenstein MR Natural IgM is required for suppression of inflammatory arthritis by apoptotic cells. J. Immunol., 2011-03-07;186(8):4967-72. 2011-03-07 [PMID: 21383247] (Flow Cytometry, Mouse)
• Llopiz D, Dotor J, Casares N, Bezunartea J, Diaz-Valdes N, Ruiz M, Aranda F, Berraondo P, Prieto J, Lasarte JJ, Borras-Cuesta F, Sarobe P Peptide inhibitors of transforming growth factor-beta enhance the efficacy of antitumor immunotherapy. Int. J. Cancer, 2009-12-01;125(11):2614-23. 2009-12-01 [PMID: 19530254] (Flow Cytometry, Mouse)

Review for CD4 Antibody (FAB554F) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Li Chou Flow Mouse 01/30/2019

Summary

• Application: Flow Cytometry
• Sample Tested: Murine Splenocytes (C57B6)
• Species: Mouse
• Lot: LGR0314031

Comments

• Comments: C57BL/6 splenocytes were isolated and stained for anti-CD4.

FAQs for CD4 Antibody (FAB554F). (Showing 1 - 1 of 1 FAQs).

1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
   • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

Bioinformatics

• Gene Symbol: CD4