CD4 Antibody (1068819) [Unconjugated]

Simple Western lane view shows lysates of Human PBMCs, loaded at 0.2 mg/mL. A specific band was detected for CD4 at approximately 65 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB11426) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Western blot shows lysates of PBMC, SUP‑T1 human T cell lymphoblastic lymphoma cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB11426) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CD4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clone: 1068819
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CD4 Antibody (1068819) [Unconjugated] Summary

• Immunogen: Chinese Hamster Ovary cell line, CHO-derived human CD4
  Lys26-Trp390
  Accession # P01730
• Specificity: Detects human CD4 in direct ELISA.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 10 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD4 Antibody (1068819) [Unconjugated]

• CD_antigen: CD4
• CD4 antigen (p55)
• CD4 antigen
• CD4 molecule
• CD4 receptor
• CD4
• CD4mut
• T-cell surface antigen T4/Leu-3
• T-cell surface glycoprotein CD4

Background

CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations (1). Mature human CD4 consists of a 371 amino acid (aa) extracellular region containing four immunoglobulin-like domains, a 22 aa transmembrane segment, and a 40 aa cytoplasmic domain (2). Within the ECD, human CD4 shares approximately 52% aa sequence identity with mouse and rat CD4. CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost, giving rise to single positive (SP) CD4⁺ or CD8⁺ mature T cells (3). CD4⁺ SP cells, also known as T helper cells, further differentiate into multiple subsets of CD4⁺ cells including Th1, Th2, Th17, Tfh, and Treg cells which regulate humoral and cellular immunity (4). CD4 is reexpressed on circulating CD8⁺ T cells upon activation and contributes to their cytotoxic effector activity (5). In human, CD4 is additionally expressed on macrophages, neutrophils, monocytes, NK cells, and neurons and glial cells in the brain (6-9). Similar CD4 distribution between species cannot be assumed as demonstrated by its presence on macrophages in human and rat but not in mouse (6). CD4 binds directly to MHC class II molecules on antigen presenting cells (10). This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction (1, 11). Palmitoylation of two cysteine residues in the cytoplasmic tail of CD4 promotes the localization of CD4 in lipid rafts and its ability to augment TCR signaling via activation of the tyrosine kinase Lck (12). CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a co-receptor for the gp120 surface glycoprotein of HIV-1 (7, 13-15).

1. Vignali, D.A.A. (2010) J. Immunol. 184:5933.
2. Maddon, P.J. et al. (1985) Cell 42:93.
3. Alarcon, B. and H.M. van Santen (2010) Sci. Signal. 3:pe11.
4. Wan, Y.Y. and R.A. Flavell (2009) Mol. Cell Biol. 1:20.
5. Kitchen, S.G. et al. (2005) Proc. Natl. Acad. Sci. 102:3794.
6. Crocker, P.R. et al. (1987) J. Exp. Med. 166:613.
7. Biswas, P. et al. (2003) Blood 101:4452.
8. Bernstein, H.B. et al. (2006) J. Immunol. 177:3669.
9. Funke, I. et al. (1987) J. Exp. Med. 165:1230.
10. Doyle, C. and J.L. Strominger (1987) Nature 330:256.
11. Huppa, J.B. et al. (2010) Nature 463:963.
12. Fragoso, R. et al. (2003) J. Immunol. 170:913.
13. Cruikshank, W.W. et al. (1994) Proc. Natl. Acad. Sci. 91:5109.
14. Klatzmann, D. et al. (1984) Nature 312:767.
15. Dagleish, A.G. et al. (1984) Nature 312:763.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for CD4 Antibody (MAB11426). (Showing 1 - 1 of 1 FAQ).

1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
   • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

Bioinformatics

• Uniprot:
  • P01730()