CD36/SR-B3 Antibody

Images

 
Western Blot: CD36/SR-B3 Antibody [NB400-144] - Detection of CD36 in human adipocyte extract (30 ug). Lane 1: 0.5 ug/ml NB 400-144; lane 2: 2 ug/ml NB 400-144. ECL: 3 second exposure.
Immunocytochemistry/ Immunofluorescence: CD36/SR-B3 Antibody [NB400-144] - Mouse liver, image is courtesy of customer review.
Immunohistochemistry-Paraffin: CD36/SR-B3 Antibody [NB400-144] - Staining of CD36 in humnan adipocytes.
Immunocytochemistry/ Immunofluorescence: CD36/SR-B3 Antibody [NB400-144] - CD36 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

CD36/SR-B3 Antibody Summary

Immunogen
A synthetic peptide mapping to a region of human CD36 between residues 100-200. [UniProt# P16671]
Localization
Integral membrane
Marker
Endothelial Cell Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CD36
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200-1:400
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200-1:400
Application Notes
This CD36 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections, and Western blot analysis where a band is seen ~75-80 kDa. The theoretical molecular weight of CD36 is ~53 kDa. The difference in theoretical MW and actual MW as seen in Western blot is most likely due to the heavy glycosylation and palmitoylation of this protein. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24531551) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
CD36/SR-B3 Lysate (NBL1-08939)
Adipose Lysate (NB820-59170)
Control Peptide
CD36/SR-B3 Peptide (NB400-144PEP)
Reviewed Applications
Read 5 Reviews rated 4
using
NB400-144 in the following applications:

Publications
Read Publications using
NB400-144 in the following applications:

Reactivity Notes

Human, bovine, mouse and monkey. Porcine reactivity reported in scientific literature (PMID: 23727393). Immunogen sequence has 95% identity to rat. Rat reactivity reported in scientific literature (PMID: 25635851)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CD36/SR-B3 Antibody

  • CD36 antigen
  • CD36 molecule (thrombospondin receptor)
  • CD36
  • Collagen R
  • FAT
  • FATCHDS7
  • Fatty acid translocase
  • Glycoprotein IIIb
  • GP3Bthrombospondin receptor)
  • GPIIIb
  • GPIV
  • Leukocyte differentiation antigen CD36
  • PAS IV
  • PAS-4
  • Platelet collagen receptor
  • platelet glycoprotein 4
  • Platelet glycoprotein IV
  • SCARB3
  • scavenger receptor class B, member 3
  • SRB3
  • SR-B3
  • Thrombospondin R
  • Thrombospondin receptor

Background

CD36 (leukocyte differentiation antigen CD36), a multifunctional protein homologous to SR-B1, is found in tissues with sustained lipid metabolism and in hematopoieic cells such as microvascular endothelium, phagocytes (macrophages, dendritic cells, microglia, and retinal pigment epithelium), erythroid precursors, hepatocytes, adipocytes, cardiac and skeletal myocytes, and specialized epithelium of the breast, kidney, gut etc. CD36 act as a negative regulator of angiogenesis via its function as a receptor for thrombospondin-1 (THBS1) and related proteins on endothelial cells. On phagocytes, through its ability to recognize specific oxidized phospholipids and lipoproteins, CD36 participates in internalization of apoptotic cells, certain bacterial/fungal pathogens, and modified LDLs, thus contributing to inflammatory responses and atherothrombotic diseases. CD36 binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption, thereby contributing to pathogenesis of diabetes/obesity. Moreover, genetic variations in CD36 are involved in susceptibility to malaria, and defects in CD36 are the cause of platelet glycoprotein IV deficiency, also called CD36 deficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD36/SR-B3 Antibody (NB400-144)(41)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ICC/IF
(1)
IHC
(3)
IHC-Fr
(1)
IHC-P
(4)
IP
(1)
WB
(24)
All Applications
Filter By Species
Human
(6)
Mouse
(23)
Rat
(3)
Porcine
(1)
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Showing Publications 1 - 10 of 41. Show All 41 Publications.
Publications using NB400-144 Applications Species
Chen X, Austin ED, Talati M et al. Oestrogen inhibition reverses pulmonary arterial hypertension and associated metabolic defects Eur. Respir. J. 2017 Aug 01 [PMID: 28775043] (WB, Mouse) WB Mouse
Chen Y, Huang W, Yang M et al. Cardiotonic Steroids Stimulate Macrophage Inflammatory Responses Through a Pathway Involving CD36, TLR4, and Na/K-ATPase. Arterioscler. Thromb. Vasc. Biol. 2017 Jun 15 [PMID: 28619997] (Mouse) Mouse
Lavie L, Dyugovskaya L, Polyakov A et al. Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro. J Vis Exp. 2017 Jan 25 [PMID: 28190059]
Garbacz WG, Lu P, Miller TM et al. Hepatic overexpression of CD36 improves glycogen homeostasis and attenuates high-fat diet induced hepatic steatosis and insulin resistance. Mol. Cell. Biol. Aug 15 2016 [PMID: 27528620] (WB, Mouse) WB Mouse
Vaillant F, Lauzier B, Ruiz M et al. Ivabradine and metoprolol differentially affect cardiac glucose metabolism despite similar heart rate reduction in a mouse model of dyslipidemia. Am. J. Physiol. Heart Circ. Physiol. Oct 1 2016 [PMID: 27496881] (WB) WB
Salameh A, Daquinag AC, Staquicini DI et al. Prohibitin/annexin 2 interaction regulates fatty acid transport in adipose tissue JCI Insight 2016 Jul 07 [PMID: 27468426]
Sachdev V, Leopold C, Bauer R et al. Novel role of a triglyceride-synthesizing enzyme: DGAT1 at the crossroad between triglyceride and cholesterol metabolism. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids Jun 23 2016 [PMID: 27344248]
Souza AC, Bocharov AV, Baranova IN et al. Antagonism of scavenger receptor CD36 by 5A peptide prevents chronic kidney disease progression in mice independent of blood pressure regulation. Kidney Int. 2016 Apr 01 [PMID: 26994575] (IHC-P, Mouse) IHC-P Mouse
Bartels ED, Christoffersen C, Lindholm MW, Nielsen LB. Altered Metabolism of LDL in the Arterial Wall Precedes Atherosclerosis Regression. Circ. Res. 2015 Sep 10 [PMID: 26358193] (IHC, Mouse) IHC Mouse
Zidek LM, Ackermann T, Hartleben G et al. Deficiency in mTORC1-controlled C/EBPbeta-mRNA translation improves metabolic health in mice. EMBO Rep 2015 Aug [PMID: 26113365] (WB, Mouse) WB Mouse
Show All 41 Publications.

Reviews for CD36/SR-B3 Antibody (NB400-144) (5) 45

Average Rating: 4
(Based on 5 reviews)
We have 5 reviews tested in 2 species: Mouse, Other.
We have 5 reviews tested in 3 application: IHC, WB, IF.

Reviews using NB400-144:
Filter by Applications
IHC
(1)
WB
(3)
IF
(1)
All Applications
Filter by Species
Mouse
(4)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry CD36/SR-B3 NB400-144
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reviewed by:
Chansol Hurr
IHC Mouse 05/11/2017
View

Summary

ApplicationImmunohistochemistry
Sample Testedmouse liver
SpeciesMouse
 
reviewed by:
Anonymous
WB Mouse 11/30/2016
View

Summary

ApplicationWestern Blot
Sample Testedcardiac tissue
SpeciesMouse

Comments

CommentsUsed antibody at a 1:1000 dilution in insoluble fraction of infarct tissue. Antibody shows many bands especially in post-infarct tissue.
Immunofluorescence CD36/SR-B3 NB400-144
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reviewed by:
Kristine DeLeon-Pennell
IF Mouse 11/30/2016
View

Summary

ApplicationImmunofluorescence
Sample Testedcardiac tissue
SpeciesMouse

Comments

CommentsCo-localization was performed using either an antibody for Mac-3 and CD36 to determine if macrophages were a major source of CD36. We used paraffin embedded infarct tissue.
 
reviewed by:
Anonymous
WB Other 12/23/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23727393
SpeciesOther

Blocking

Blocking DetailsSee PMID 23727393

Details

Detection NotesSee PMID 23727393

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 23727393.
Western Blot CD36/SR-B3 NB400-144
Enlarge
reviewed by:
Cathal McCarthy
WB Mouse 02/09/2011
View

Summary

ApplicationWestern Blot
Sample TestedWhole tissue lysate ApoE murine aorta, Sample Amount: 20ug
SpeciesMouse
LotC2
CommentsExcellent antibody, works very well on both macrophages and in vivo murine aorta

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in TBST, Blocking Time: 2 hours, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Incubation Dilution Buffer: 5% milk in TBS-T, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: goat anti-rabbit , Secondary Ab Dilution Ratio: 1:10000
Secondary Manufacturer Cat#sc-2004 Santa Cruz

Details

Detection NotesDetection Method: Supersignal Pierce, Exposure Time: 10 min, Specific Bands: 75kDa, Non-Specific Bands: None

Comments

CommentsExcellent antibody, works very well on both macrophages and in vivo murine aorta

Product General Protocols

View specific protocols for CD36/SR-B3 Antibody (NB400-144): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD36/SR-B3 Antibody (NB400-144). (Showing 1 - 2 of 2 FAQs).

  1. Can you tell me if this product, NB400-144, requires antigen retrieval for hick on paraffin embedded tissues? If so, what type of antigen retrieval is recommended?
    • Yes, we recommend antigen retrieval for IHC-P with NB400-144 and in our testing we performed heat-mediated antigen retrieval with sodium citrate buffer (pH 6).
  2. We used the NB400-144 to detect CD36 in guinea pig by Western blot analysis. Is there a similar antibody recommended for IP?
    • Here is a link to other CD36 antibodies which are validated for IP. The CD36 antibody (NB400-144) that you already have is one of our best selling products with over 15 PubMed citations. We have not tested this antibody in IP yet and I do not see any reason why it would not work in IP. If you would be interested in testing this novel application, please take a look at our Innovator's Reward program.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB400-144AF405
Alexa Fluor 488 Labeled NB400-144AF488
Alexa Fluor 647 Labeled NB400-144AF647
Alexa Fluor 700 Labeled NB400-144AF700
Biotin Labeled NB400-144B
DyLight 405 Labeled NB400-144V
DyLight 488 Labeled NB400-144G
DyLight 550 Labeled NB400-144R
DyLight 650 Labeled NB400-144C
FITC Labeled NB400-144F
HRP Labeled NB400-144H

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Recent Reviews

4
5
1
4
3
3
1
2
0
1
0

Chansol Hurr
05/11/2017
Application: IHC
Species: Mouse

 
Anonymous
11/30/2016
Application: WB
Species: Mouse

Kristine DeLeon-Pennell
11/30/2016
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol CD36
Entrez