CD36/SR-B3 Antibody

Images

 
Western Blot: CD36/SR-B3 Antibody [NB400-144] - Total protein from Human Skin and Adipose tissue, Mouse Adipose and Rat Adipose tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in ...read more
Western Blot: CD36/SR-B3 Antibody [NB400-144] - Detection of CD36 in human adipocyte extract (30 ug). Lane 1: 0.5 ug/ml NB 400-144; lane 2: 2 ug/ml NB 400-144. ECL: 3 second exposure.
Immunocytochemistry/ Immunofluorescence: CD36/SR-B3 Antibody [NB400-144] - CD36 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 ...read more
Western Blot: CD36/SR-B3 Antibody [NB400-144] - Alcohol feeding significantly up-regulated CD36 expression. Image from verified customer review.
Immunohistochemistry-Paraffin: CD36/SR-B3 Antibody [NB400-144] - Staining of CD36 in human adipocytes.
Western Blot: CD36/SR-B3 Antibody [NB400-144] - Mouse primary muscle cell lysate. This image was submitted via customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

CD36/SR-B3 Antibody Summary

Immunogen
A synthetic peptide mapping to a region of human CD36 between residues 100-200. [UniProt# P16671]
Localization
Integral membrane
Marker
Endothelial Cell Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CD36
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200-1:400
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200-1:400
Application Notes
This CD36 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections, and Western blot analysis where a band is seen ~75-80 kDa. The theoretical molecular weight of CD36 is ~53 kDa. The difference in theoretical MW and actual MW as seen in Western blot is most likely due to the heavy glycosylation and palmitoylation of this protein. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 24531551). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CD36/SR-B3 Lysate (NBL1-08939)
Adipose Lysate (NB820-59170)
Control Peptide
CD36/SR-B3 Peptide (NB400-144PEP)
Reviewed Applications
Read 7 Reviews rated 4.3
using
NB400-144 in the following applications:

Publications
Read Publications using
NB400-144 in the following applications:

  • 2 publications
  • IHC
    3 publications
  • 3 publications
  • 4 publications
  • IP
    1 publication
  • WB
    29 publications

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 23727393). Rat reactivity reported in scientific literature (PMID: 25635851)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CD36/SR-B3 Antibody

  • CD36 antigen
  • CD36 molecule (thrombospondin receptor)
  • CD36
  • Collagen R
  • FAT
  • FATCHDS7
  • Fatty acid translocase
  • Glycoprotein IIIb
  • GP3Bthrombospondin receptor)
  • GPIIIb
  • GPIV
  • Leukocyte differentiation antigen CD36
  • PAS IV
  • PAS-4
  • Platelet collagen receptor
  • platelet glycoprotein 4
  • Platelet glycoprotein IV
  • SCARB3
  • scavenger receptor class B, member 3
  • SRB3
  • SR-B3
  • Thrombospondin R
  • Thrombospondin receptor

Background

CD36 (leukocyte differentiation antigen CD36), a multifunctional protein homologous to SR-B1, is found in tissues with sustained lipid metabolism and in hematopoieic cells such as microvascular endothelium, phagocytes (macrophages, dendritic cells, microglia, and retinal pigment epithelium), erythroid precursors, hepatocytes, adipocytes, cardiac and skeletal myocytes, and specialized epithelium of the breast, kidney, gut etc. CD36 act as a negative regulator of angiogenesis via its function as a receptor for thrombospondin-1 (THBS1) and related proteins on endothelial cells. On phagocytes, through its ability to recognize specific oxidized phospholipids and lipoproteins, CD36 participates in internalization of apoptotic cells, certain bacterial/fungal pathogens, and modified LDLs, thus contributing to inflammatory responses and atherothrombotic diseases. CD36 binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption, thereby contributing to pathogenesis of diabetes/obesity. Moreover, genetic variations in CD36 are involved in susceptibility to malaria, and defects in CD36 are the cause of platelet glycoprotein IV deficiency, also called CD36 deficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB400-104
Species: Hu, Mu, Rt, ChHa, SyHa, Ha, Md, Pm, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-84550
Species: Hu
Applications: WB, IHC, IHC-P
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NBP2-22106
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB300-537
Species: Hu, Mu, Rt, Bv, Rb
Applications: WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP
NB100-2564
Species: Hu
Applications: WB
NBP2-01743
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF7197
Species: Hu, Mu
Applications: WB, IHC, ICC
7270-IL/CF
Species: Hu
H00376497-D01P
Species: Hu, Mu
Applications: WB, ELISA
NBP1-00092
Species: Hu, Mu
Applications: WB, ICC/IF, IHC-Fr, PLA
NBP2-66200
Species: Hu
NBP2-16708
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB400-144
Species: Hu, Mu, Rt, Po, Bv, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for CD36/SR-B3 Antibody (NB400-144)(50)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ICC/IF
(2)
IHC
(3)
IHC-Fr
(3)
IHC-P
(4)
IP
(1)
WB
(29)
All Applications
Filter By Species
Human
(8)
Mouse
(30)
Rat
(3)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 50. Show All 50 Publications.
Publications using NB400-144 Applications Species
Lindsey ML, Jung M, Yabluchanskiy A et al. Exogenous CXCL4 Infusion Inhibits Macrophage Phagocytosis by Limiting CD36 Signaling to Enhance Post-myocardial Infarction Cardiac Dilation and Mortality. Cardiovasc. Res. Aug 29 2018 [PMID: 30169632] (WB, Human) WB Human
Fang Y, Wang J, Yao L et al. The adhesion and migration of microglia to B-amyloid (AB) is decreased with aging and inhibited by Nogo/NgR pathway. J Neuroinflammation. Jul 20 2018 [PMID: 30029608] (WB, Mouse) WB Mouse
Li Y, Lu Z, Ru JH et al. Saturated Fatty Acid Combined with Lipopolysaccharide Stimulates a Strong Inflammatory Response in Hepatocytes in vivo and in vitro. Am. J. Physiol. Endocrinol. Metab. Jul 10 2018 [PMID: 29989851] (IHC-Fr, Mouse) IHC-Fr Mouse
Sankar SB, Donegan RK, Shah KJ et al. Heme and hemoglobin suppress amyloid b-mediated inflammatory activation of mouse astrocytes J. Biol. Chem. Jun 5 2018 [PMID: 29871926] (WB, Mouse) WB Mouse
Misra A, Feng Z, Chandran RR et al. Integrin beta3 regulates clonality and fate of smooth muscle-derived atherosclerotic plaque cells Nat Commun May 25 2018 [PMID: 29802249] (IHC-Fr, Mouse) IHC-Fr Mouse
Rozovski U, Harris DM, Li P et al. STAT3-activated CD36 facilitates fatty acid uptake in chronic lymphocytic leukemia cells Oncotarget Apr 20 2018 [PMID: 29765537] (ICC/IF, Human) ICC/IF Human
Lee JY, Han SH, Park MH et al. Neuronal SphK1 acetylates COX2 and contributes to pathogenesis in a model of Alzheimer's Disease. Nat Commun Apr 16 2018 [PMID: 29662056] (WB, Mouse) WB Mouse
Guri Y, Colombi M, Dazert E et al. mTORC2 Promotes Tumorigenesis via Lipid Synthesis. Cancer Cell 2017 Dec 11 [PMID: 29232555] (Mouse) Mouse
Xian X, Ding Y, Dieckmann M et al. LRP1 integrates murine macrophage cholesterol homeostasis and inflammatory responses in atherosclerosis Elife. 2017 Nov 16 [PMID: 29144234] (WB, Mouse) WB Mouse
Chen X, Austin ED, Talati M et al. Oestrogen inhibition reverses pulmonary arterial hypertension and associated metabolic defects Eur. Respir. J. 2017 Aug 01 [PMID: 28775043] (WB, Mouse) WB Mouse
Show All 50 Publications.

Reviews for CD36/SR-B3 Antibody (NB400-144) (7) 4.37

Average Rating: 4.3
(Based on 7 reviews)
We have 7 reviews tested in 2 species: Mouse, Other.

Reviews using NB400-144:
Filter by Applications
WB
(5)
IHC
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(6)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot CD36/SR-B3 NB400-144
Enlarge
reviewed by:
Marie-Claude Sincennes
WB Mouse 03/13/2018
View

Summary

ApplicationWestern Blot
Sample TestedPrimary muscle cell lysate
SpeciesMouse
LotJ-3
Western Blot CD36/SR-B3 NB400-144
Enlarge
reviewed by:
Anonymous
WB Mouse 03/01/2018
View

Summary

ApplicationWestern Blot
Sample Testedwhite adipose tissue
SpeciesMouse
Lotf-5
Immunohistochemistry CD36/SR-B3 NB400-144
Enlarge
reviewed by:
Chansol Hurr
IHC Mouse 05/11/2017
View

Summary

ApplicationImmunohistochemistry
Sample Testedmouse liver
SpeciesMouse
 
reviewed by:
Anonymous
WB Mouse 11/30/2016
View

Summary

ApplicationWestern Blot
Sample Testedcardiac tissue
SpeciesMouse

Comments

CommentsUsed antibody at a 1:1000 dilution in insoluble fraction of infarct tissue. Antibody shows many bands especially in post-infarct tissue.
Immunofluorescence CD36/SR-B3 NB400-144
Enlarge
reviewed by:
Kristine DeLeon-Pennell
IF Mouse 11/30/2016
View

Summary

ApplicationImmunofluorescence
Sample Testedcardiac tissue
SpeciesMouse

Comments

CommentsCo-localization was performed using either an antibody for Mac-3 and CD36 to determine if macrophages were a major source of CD36. We used paraffin embedded infarct tissue.
 
reviewed by:
Anonymous
WB Other 12/23/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23727393
SpeciesOther

Blocking

Blocking DetailsSee PMID 23727393

Details

Detection NotesSee PMID 23727393

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 23727393.
Western Blot CD36/SR-B3 NB400-144
Enlarge
reviewed by:
Cathal McCarthy
WB Mouse 02/09/2011
View

Summary

ApplicationWestern Blot
Sample TestedWhole tissue lysate ApoE murine aorta, Sample Amount: 20ug
SpeciesMouse
LotC2
CommentsExcellent antibody, works very well on both macrophages and in vivo murine aorta

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in TBST, Blocking Time: 2 hours, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Incubation Dilution Buffer: 5% milk in TBS-T, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: goat anti-rabbit, Secondary Ab Dilution Ratio: 1:10000
Secondary Manufacturer Cat#sc-2004 Santa Cruz

Details

Detection NotesDetection Method: Supersignal Pierce, Exposure Time: 10 min, Specific Bands: 75kDa, Non-Specific Bands: None

Comments

CommentsExcellent antibody, works very well on both macrophages and in vivo murine aorta

Product General Protocols

View specific protocols for CD36/SR-B3 Antibody (NB400-144): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD36/SR-B3 Antibody (NB400-144). (Showing 1 - 2 of 2 FAQs).

  1. Can you tell me if this product, NB400-144, requires antigen retrieval for hick on paraffin embedded tissues? If so, what type of antigen retrieval is recommended?
    • Yes, we recommend antigen retrieval for IHC-P with NB400-144 and in our testing we performed heat-mediated antigen retrieval with sodium citrate buffer (pH 6).
  2. We used the NB400-144 to detect CD36 in guinea pig by Western blot analysis. Is there a similar antibody recommended for IP?
    • Here is a link to other CD36 antibodies which are validated for IP. The CD36 antibody (NB400-144) that you already have is one of our best selling products with over 15 PubMed citations. We have not tested this antibody in IP yet and I do not see any reason why it would not work in IP. If you would be interested in testing this novel application, please take a look at our Innovator's Reward program.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB400-144AF405
Alexa Fluor 488 Labeled NB400-144AF488
Alexa Fluor 647 Labeled NB400-144AF647
Alexa Fluor 700 Labeled NB400-144AF700
Biotin Labeled NB400-144B
DyLight 405 Labeled NB400-144V
DyLight 488 Labeled NB400-144G
DyLight 550 Labeled NB400-144R
DyLight 650 Labeled NB400-144C
FITC Labeled NB400-144F
HRP Labeled NB400-144H

Additional Array Products

Bioinformatics Tool for CD36/SR-B3 Antibody (NB400-144)

Discover related pathways, diseases and genes to CD36/SR-B3 Antibody (NB400-144). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CD36/SR-B3 Antibody (NB400-144)

Discover more about diseases related to CD36/SR-B3 Antibody (NB400-144).
 

Pathways for CD36/SR-B3 Antibody (NB400-144)

View related products by pathway.

PTMs for CD36/SR-B3 Antibody (NB400-144)

Learn more about PTMs related to CD36/SR-B3 Antibody (NB400-144).
 

Research Areas for CD36/SR-B3 Antibody (NB400-144)

Find related products by research area.

Blogs on CD36/SR-B3

There are no specific blogs for CD36/SR-B3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.3
5
3
4
3
3
1
2
0
1
0

Marie-Claude Sincennes
03/13/2018
Application: WB
Species: Mouse

Anonymous
03/01/2018
Application: WB
Species: Mouse

Chansol Hurr
05/11/2017
Application: IHC
Species: Mouse

Bioinformatics

Gene Symbol CD36