CD36/SR-B3 Antibody


Western Blot: CD36/SR-B3 Antibody [NB400-144] - Detection of CD36 in human adipocyte extract (30 ug). Lane 1: 0.5 ug/ml NB 400-144; lane 2: 2 ug/ml NB 400-144. ECL: 3 second exposure.
Immunocytochemistry/ Immunofluorescence: CD36/SR-B3 Antibody [NB400-144] - CD36 antibody was tested in HepG2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 more
Immunohistochemistry-Paraffin: CD36/SR-B3 Antibody [NB400-144] - Staining of CD36 in humnan adipocytes.

Product Details

Reactivity Hu, Mu, Rt, Po, Bv, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
1 mg/ml

CD36/SR-B3 Antibody Summary

A synthetic peptide mapping to a region of human CD36 between residues 100-200. [UniProt# P16671]
Integral membrane
Endothelial Cell Marker
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1 mg/ml
Immunogen affinity purified


  • Western Blot 1:500-1:2000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200-1:400
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200-1:400
Application Notes
This CD36 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections, and Western blot analysis where a band is seen ~75-80 kDa. The theoretical molecular weight of CD36 is ~53 kDa. The difference in theoretical MW and actual MW as seen in Western blot is most likely due to the heavy glycosylation and palmitoylation of this protein. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24531551)

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
CD36/SR-B3 Peptide (NB400-144PEP)
Reviewed Applications
Read 2 Reviews rated 4.5
NB400-144 in the following applications:

Read Publications using
NB400-144 in the following applications:

Reactivity Notes

Human, bovine, mouse and monkey. Porcine reactivity reported in scientific literature (PMID: 23727393). Immunogen sequence has 95% identity to rat. Rat reactivity reported in scientific literature (PMID: 25635851)

Alternate Names for CD36/SR-B3 Antibody

  • CD36 antigen
  • CD36 molecule (thrombospondin receptor)
  • CD36
  • Collagen R
  • FAT
  • Fatty acid translocase
  • Glycoprotein IIIb
  • GP3Bthrombospondin receptor)
  • GPIV
  • Leukocyte differentiation antigen CD36
  • PAS IV
  • PAS-4
  • Platelet collagen receptor
  • platelet glycoprotein 4
  • Platelet glycoprotein IV
  • SCARB3
  • scavenger receptor class B, member 3
  • SRB3
  • SR-B3
  • Thrombospondin R
  • Thrombospondin receptor


CD36 (leukocyte differentiation antigen CD36), a multifunctional protein homologous to SR-B1, is found in tissues with sustained lipid metabolism and in hematopoieic cells such as microvascular endothelium, phagocytes (macrophages, dendritic cells, microglia, and retinal pigment epithelium), erythroid precursors, hepatocytes, adipocytes, cardiac and skeletal myocytes, and specialized epithelium of the breast, kidney, gut etc. CD36 act as a negative regulator of angiogenesis via its function as a receptor for thrombospondin-1 (THBS1) and related proteins on endothelial cells. On phagocytes, through its ability to recognize specific oxidized phospholipids and lipoproteins, CD36 participates in internalization of apoptotic cells, certain bacterial/fungal pathogens, and modified LDLs, thus contributing to inflammatory responses and atherothrombotic diseases. CD36 binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption, thereby contributing to pathogenesis of diabetes/obesity. Moreover, genetic variations in CD36 are involved in susceptibility to malaria, and defects in CD36 are the cause of platelet glycoprotein IV deficiency, also called CD36 deficiency.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, ChHa, SyHa, Ha, Md, Pm, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P
Species: Hu, Mu, Rt, Bv, Rb
Applications: WB, Flow, GS, IHC, IHC-P, IP
Species: Hu, Pm
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ICC
Species: Hu
Applications: ICC, ICFlow
Species: Hu, Mu
Applications: WB, ICC/IF, IHC-Fr
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for CD36/SR-B3 Antibody (NB400-144)(35)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 35. Show All 35 Publications.
Publications using NB400-144 Applications Species
Salameh A, Daquinag AC, Staquicini DI et al. Prohibitin/annexin 2 interaction regulates fatty acid transport in adipose tissue JCI Insight 2016 Jul 07 [PMID:27468426]
Souza AC, Bocharov AV, Baranova IN et al. Antagonism of scavenger receptor CD36 by 5A peptide prevents chronic kidney disease progression in mice independent of blood pressure regulation. Kidney Int. 2016 Apr 01 [PMID:26994575] (IHC-P, Mouse) IHC-P Mouse
Bartels ED, Christoffersen C, Lindholm MW, Nielsen LB. Altered Metabolism of LDL in the Arterial Wall Precedes Atherosclerosis Regression. Circ. Res. 2015 Sep 10 [PMID:26358193] (IHC, Mouse) IHC Mouse
Zidek LM, Ackermann T, Hartleben G et al. Deficiency in mTORC1-controlled C/EBPbeta-mRNA translation improves metabolic health in mice. EMBO Rep 2015 Aug [PMID:26113365] (WB, Mouse) WB Mouse
Barroso E, Rodriguez-Rodriguez R, Chacon MR et al. PPARbeta/delta ameliorates fructose-induced insulin resistance in adipocytes by preventing Nrf2 activation Biochim. Biophys. Acta 2015 May 01 [PMID:25728706] (WB, Mouse) WB Mouse
Konstantynowicz-Nowicka K, Harasim E, Baranowski M, Chabowski A. New evidence for the role of ceramide in the development of hepatic insulin resistance PLoS ONE. 2015 Jan 31 [PMID:25635851] (WB, Rat) WB Rat
Ye R, Holland Wl, Gordillo R et al. Adiponectin is essential for lipid homeostasis and survival under insulin deficiency and promotes B-cell regeneration. Elife. 2014 Oct 23 [PMID:25339419] (IHC, Mouse) IHC Mouse
Srikanthan S, Li W, Silverstein RL, McIntyre TM. Exosome poly-ubiquitin inhibits platelet activation, downregulates CD36, and inhibits pro-atherothombotic cellular functions. J Thromb. Haemost. 2014 Aug 28 [PMID:25163645]
Chen Jingjing, Yong Yangyang, Xia Xian et al. The excreted polysaccharide of Pleurotus eryngii inhibits the foam-cell formation via down-regulation of CD36. Carbohydr Polym. 2014 Nov 04 [PMID:25129711]
Hale JS, Otvos B, Sinyuk M et al. Cancer stem cell-specific scavenger receptor CD36 drives glioblastoma progression. Stem Cells 2014 Apr 15 [PMID:24737733] (IHC-P, Human) IHC-P Human
Show All 35 Publications.

Reviews for CD36/SR-B3 Antibody (NB400-144) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Other.
We have 2 review tested in 1 application: WB.

Reviews using NB400-144:
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Images Ratings Applications Species Date Details
reviewed by:
WB Other 12/23/2014


ApplicationWestern Blot
Sample TestedSee PMID 23727393


Blocking DetailsSee PMID 23727393


Detection NotesSee PMID 23727393


CommentsIndependent review from antibodyresource.comPublished in PMID 23727393.
Western Blot CD36/SR-B3 NB400-144
reviewed by:
WB Mouse 02/09/2011


ApplicationWestern Blot
Sample TestedWhole tissue lysate ApoE murine aorta, Sample Amount: 20ug
CommentsExcellent antibody, works very well on both macrophages and in vivo murine aorta


Blocking DetailsBlocking Buffer: 5% Milk in TBST, Blocking Time: 2 hours, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Incubation Dilution Buffer: 5% milk in TBS-T, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: goat anti-rabbit , Secondary Ab Dilution Ratio: 1:10000
Secondary Manufacturer Cat#sc-2004 Santa Cruz


Detection NotesDetection Method: Supersignal Pierce, Exposure Time: 10 min, Specific Bands: 75kDa, Non-Specific Bands: None


CommentsExcellent antibody, works very well on both macrophages and in vivo murine aorta

Product General Protocols

View specific protocols for CD36/SR-B3 Antibody (NB400-144): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD36/SR-B3 Antibody (NB400-144). (Showing 1 - 2 of 2 FAQs).

  1. Can you tell me if this product, NB400-144, requires antigen retrieval for hick on paraffin embedded tissues? If so, what type of antigen retrieval is recommended?
    • Yes, we recommend antigen retrieval for IHC-P with NB400-144 and in our testing we performed heat-mediated antigen retrieval with sodium citrate buffer (pH 6).
  2. We used the NB400-144 to detect CD36 in guinea pig by Western blot analysis. Is there a similar antibody recommended for IP?
    • Here is a link to other CD36 antibodies which are validated for IP. The CD36 antibody (NB400-144) that you already have is one of our best selling products with over 15 PubMed citations. We have not tested this antibody in IP yet and I do not see any reason why it would not work in IP. If you would be interested in testing this novel application, please take a look at our Innovator's Reward program.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB400-144AF405
Alexa Fluor (R) 488 Labeled NB400-144AF488
Alexa Fluor (R) 647 Labeled NB400-144AF647
Alexa Fluor (R) 700 Labeled NB400-144AF700
Biotin Labeled NB400-144B
DyLight 405 Labeled NB400-144V
DyLight 488 Labeled NB400-144G
DyLight 550 Labeled NB400-144R
DyLight 650 Labeled NB400-144C
FITC Labeled NB400-144F
HRP Labeled NB400-144H

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Gene Symbol CD36

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