GOT2 Antibody

Immunocytochemistry/Immunofluorescence: GOT2 Antibody [NBP2-16708] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-16708] - Mouse brain. GOT2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: GOT2 Antibody [NBP2-16708] - 50 ug rat lung lysate/extract 10 % SDS-PAGE GOT2 antibody dilution: 1:1000.

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-16708] - Hepatoma tissue. GOT2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-16708] - RT2 xenograft, using GOT2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: GOT2 Antibody [NBP2-16708] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GOT2 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

GOT2 antibody detects GOT2 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. GOT2 antibody (NBP2-16708) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GOT2 antibody detects GOT2 protein by western blot analysis.A. 30 ug Neuro2A whole cell lysate/extract 10% SDS-PAGEGOT2 antibody (NBP2-16708) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE NBP2-16708 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ICC/IF, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

GOT2 Antibody Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit GOT2 Antibody (NBP2-16708) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-GOT2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human GOT2. The exact sequence is proprietary.
• Localization: Mitochondrion matrix; Cell membrane
• Predicted Species: Bovine (90%), Canine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GOT2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (81%), Porcine (89%), Chicken (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for GOT2 Antibody

• aspartate aminotransferase, mitochondrial
• EC 2.6.1
• EC 2.6.1.1
• FABP-1
• FABPpm
• Fatty acid-binding protein
• FLJ40994
• Glutamate oxaloacetate transaminase 2
• glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase2)
• KAT4
• KATIV
• kynurenine aminotransferase IV
• mAspAT
• mitAAT
• Plasma membrane-associated fatty acid-binding protein
• Transaminase A

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for GOT2 Antibody (NBP2-16708)(2)

Publications using NBP2-16708

• Islam M, Umehara T, Tsujita N, Shimada M Saturated fatty acids accelerate linear motility through mitochondrial ATP production in bull sperm Reprod Med Biol 2021-07-15 [PMID: 34262396]
• Zabielski P, Hady HR, Chacinska M et al. The effect of high fat diet and metformin treatment on liver lipids accumulation and their impact on insulin action Sci Rep 2018-05-08 [PMID: 29739997] (WB, Rat)

Bioinformatics

• Gene Symbol: GOT2
• Uniprot:
  • P00505(Human)