GOT2 Antibody

Western Blot: GOT2 Antibody [NBP2-16708] - 30 ug Neuro2A whole cell lysate/extract 10 % SDS-PAGE GOT2 antibody dilution: 1:1000. (Mouse)

Immunocytochemistry/Immunofluorescence: GOT2 Antibody [NBP2-16708] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-16708] - Paraffin-embedded mouse brain GOT2 antibody diluted at 1:500.

Western Blot: GOT2 Antibody [NBP2-16708] - 50 ug rat lung lysate/extract 10 % SDS-PAGE GOT2 antibody dilution: 1:1000.

Western Blot: GOT2 Antibody [NBP2-16708] - Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-16708] - GOT2 antibody detects GOT2 protein at cytosol on hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma tissue. GOT2 antibody dilution: 1:500.

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-16708] - GOT2 antibody detects GOT2 protein at cytosol on SkHep1 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SkHep1 xenograft. GOT2 antibody dilution: 1:500.

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-16708] - Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-16708] - Paraffin-embedded mouse liver diluted at 1:500.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

GOT2 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human GOT2. The exact sequence is proprietary.
• Localization: Mitochondrion matrix; Cell membrane
• Predicted Species: Bovine (90%), Canine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GOT2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (81%), Porcine (89%), Chicken (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for GOT2 Antibody

• aspartate aminotransferase, mitochondrial
• EC 2.6.1
• EC 2.6.1.1
• FABP-1
• FABPpm
• Fatty acid-binding protein
• FLJ40994
• Glutamate oxaloacetate transaminase 2
• glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase2)
• KAT4
• KATIV
• kynurenine aminotransferase IV
• mAspAT
• mitAAT
• Plasma membrane-associated fatty acid-binding protein
• Transaminase A

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: GOT2
• Uniprot:
  • P00505(Human)