SR-AI/MSR Antibody [Unconjugated]

CKIP-1 diminishes Oct-1-mediated LOX-1 expression. e Expression of LOX-1, CD36, and SR-A in Ckip-1−/− and Ckip-1−/−R BMDMs. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30683852), licensed under a CC-BY license. Not internally tested by R&D Systems.

CKIP-1 diminishes Oct-1-mediated LOX-1 expression. c Expression of LOX-1, CD36, and SR-A in WT and Ckip-1−/− BMDMs after 24 h incubation with or without 20 μg per ml oxLDL. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30683852), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effects of kcnq1ot1 on cholesterol transporter and receptor expression. A, B The mRNA and protein levels of ABCA1, ABCG1, CD36, and SR-A in the aorta from apoE−/− mice were assayed by qRT-PCR and western blot, respectively (n = 10). C, D qRT-PCR and western blot analyses of ABCA1, ABCG1, CD36 and SR-A expression in MPMs from apoE−/− mice (n = 5). E, F After treatment of THP-1 macrophages with PBS, LV-NC or LV-kcnq1ot1 for 72 h, both qRT-PCR and western blot were employed for detection of ABCA1, ABCG1, CD36, and SR-A expressions. Data are represented as mean ± SD. ***P < 0.001; ns not significant. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33293505), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effects of kcnq1ot1 on cholesterol transporter and receptor expression. A, B The mRNA and protein levels of ABCA1, ABCG1, CD36, and SR-A in the aorta from apoE−/− mice were assayed by qRT-PCR and western blot, respectively (n = 10). C, D qRT-PCR and western blot analyses of ABCA1, ABCG1, CD36 and SR-A expression in MPMs from apoE−/− mice (n = 5). E, F After treatment of THP-1 macrophages with PBS, LV-NC or LV-kcnq1ot1 for 72 h, both qRT-PCR and western blot were employed for detection of ABCA1, ABCG1, CD36, and SR-A expressions. Data are represented as mean ± SD. ***P < 0.001; ns not significant. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33293505), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

SR-AI/MSR Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse SR-AI/MSR
  Trp79-Ser454
  Accession # AAA39747
• Specificity: Detects mouse SR-A1/MSR in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MSR1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SR-AI/MSR Antibody [Unconjugated]

• CD204 antigen
• CD204
• Macrophage acetylated LDL receptor I and II
• macrophage scavenger receptor 1
• macrophage scavenger receptor type III
• MSR1
• phSR1
• phSR2
• SCARA1
• SCARA1macrophage scavenger receptor types I and II
• Scavenger receptor class A member 1
• scavenger receptor class A, member 1
• SR-A
• SRAI
• SR-AI

Background

The scavenger receptor (SR) family comprises a group of functionally defined membrane receptors that share the common ability to bind and internalize modified forms of Low Density Lipoproteins (mLDL) (1 - 3). Family members are classified alphabetically. The A class include four proteins: the three subtypes of SR-A (AI, AII, and AIII) that are generated by alternative splicing of the same gene, and a structurally similar protein named MARCO (4). All A class SRs are multidomain trimeric type II membrane proteins. SR-AI has an N-terminal cytoplasmic domain, a transmembrane domain, a spacer domain, an alpha -helical coiled coil, a collagen-like domain and a C-terminal cysteine-rich domain. SR-A is expressed by most tissue macrophages, dendritic cells and Kupffer cells. It is also highly expressed by microglia in neonatal as well as Alzheimer’ Disease brains. SR-AI binds a broad range of polyanionic ligands including modified proteins (e.g. Oxidized, acetylated or maleylated LDL, Advanced glycation end-product proteins), polyribonucleotides (polyguanosine and polyinosine), polysaccharides (dextran sulfate, fucoidan), phospholipids (phosphatidylserine), bacterial products (lipopolysaccharide and lipoteichoic acid) and selected chemical compounds (silica, crocidolite asbestos). The ligand-binding region has been localized to a positively charged region in the carboxyl end of the collagen-like domain. Based on its ligand binding characteristics, SR-AI is implicated in many physiological and pathophysiological functions. Studies using SR-A knockout mouse have also suggested roles of SR-A in atherogenesis, host defense and innate immunity, acquired immune responses, macrophage adhesion, and phagocytosis of apoptotic cells (1 - 3).

1. Daugherty, A. et al. (2000) Curr. Opin. Cardiovasc. Pulm. Ren. Invest. Drugs 2:223.
2. Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649.
3. Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193.
4. Elomaa, O. et al. (1995) Cell 80:603.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SR-AI/MSR Antibody (AF1797)(17)

Publications using AF1797

• Shen, C;Zhu, X;Chang, H;Li, C;Hou, M;Chen, L;Lu Chen, ;Zhou, Z;Ji, M;Xu, Z; The rebalancing of the immune system at the maternal-fetal interface ameliorates autism-like behavior in adult offspring Cell reports 2024-09-24 [PMID: 39321022] (Western Blot, Mouse)
• Xiao-Hua Yu, Jiao-Jiao Chen, Wen-Yi Deng, Xiao-Dan Xu, Qi-Xian Liu, Meng-Wen Shi et al. Biochanin A Mitigates Atherosclerosis by Inhibiting Lipid Accumulation and Inflammatory Response Oxidative Medicine and Cellular Longevity 11/11/2020 [PMID: 33959213]
• A Kitaura, T Nishinaka, S Hamasaki, OF Hatipoglu, H Wake, M Nishibori, S Mori, S Nakao, H Takahashi Advanced glycation end-products reduce lipopolysaccharide uptake by macrophages PLoS ONE, 2021-01-25;16(1):e0245957. 2021-01-25 [PMID: 33493233] (Neutralization, Mouse)
• XH Yu, WY Deng, JJ Chen, XD Xu, XX Liu, L Chen, MW Shi, QX Liu, M Tao, K Ren LncRNA kcnq1ot1 promotes lipid accumulation and accelerates atherosclerosis via functioning as a ceRNA through the miR-452-3p/HDAC3/ABCA1 axis Cell Death & Disease, 2020-12-09;11(12):1043. 2020-12-09 [PMID: 33293505] (Western Blot, Human)
• Long Yang, Tingting Geng, Guang Yang, Jinzhu Ma, Leilei Wang, Harshada Ketkar et al. Macrophage scavenger receptor 1 controls Chikungunya virus infection through autophagy in mice Communications Biology 10/8/2020 [PMID: 33033362]
• F Hu, X Jiang, C Guo, Y Li, S Chen, W Zhang, Y Du, P Wang, X Zheng, X Fang, X Li, J Song, Y Xie, F Huang, J Xue, M Bai, Y Jia, X Liu, L Ren, X Zhang, J Guo, H Pan, Y Su, H Yi, H Ye, D Zuo, J Li, H Wu, Y Wang, R Li, L Liu, XY Wang, Z Li Scavenger receptor-A is a biomarker and effector of rheumatoid arthritis: A large-scale multicenter study Nat Commun, 2020-04-20;11(1):1911. 2020-04-20 [PMID: 32312978] (In Vivo, Mouse)
• Guankui Wang, Natalie J. Serkova, Ernest V. Groman, Robert I. Scheinman, Dmitri Simberg Feraheme (Ferumoxytol) Is Recognized by Proinflammatory and Anti-inflammatory Macrophages via Scavenger Receptor Type AI/II Molecular Pharmaceutics 10/7/2019 [PMID: 31556296]
• Stella J. Berendam, Alexander F. Koeppel, Nicole R. Godfrey, Sherin J. Rouhani, Amber N. Woods, Anthony B. Rodriguez et al. Comparative Transcriptomic Analysis Identifies a Range of Immunologically Related Functional Elaborations of Lymph Node Associated Lymphatic and Blood Endothelial Cells Frontiers in Immunology 4/16/2019 [PMID: 31057546]
• S Hamasaki, T Kobori, Y Yamazaki, A Kitaura, A Niwa, T Nishinaka, M Nishibori, S Mori, S Nakao, H Takahashi Effects of scavenger receptors-1 class A stimulation on macrophage morphology and highly modified advanced glycation end product-protein phagocytosis Sci Rep, 2018-04-12;8(1):5901. 2018-04-12 [PMID: 29651042] (Neutralization, Mouse)
• S Komine, K Akiyama, E Warabi, S Oh, K Kuga, K Ishige, S Togashi, T Yanagawa, J Shoda Exercise training enhances in vivo clearance of endotoxin and attenuates inflammatory responses by potentiating Kupffer cell phagocytosis Sci Rep, 2017-09-20;7(1):11977. 2017-09-20 [PMID: 28931917] (Flow Cytometry, Mouse)
• Bonilla D, Bhattacharya A, Sha Y, Xu Y, Xiang Q, Kan A, Jagannath C, Komatsu M, Eissa N Autophagy regulates phagocytosis by modulating the expression of scavenger receptors. Immunity, 2013-09-12;39(3):537-47. 2013-09-12 [PMID: 24035364] (Flow Cytometry, Mouse)
• The receptor of advanced glycation end products plays a central role in advanced oxidation protein products-induced podocyte apoptosis. Kidney Int, 2012-05-23;82(7):759-70. 2012-05-23 [PMID: 22622498] (Neutralization, Mouse)
• Qian J, Yi H, Guo C, Yu X, Zuo D, Chen X, Kane JM, Repasky EA, Subjeck JR, Wang XY CD204 suppresses large heat shock protein-facilitated priming of tumor antigen gp100-specific T cells and chaperone vaccine activity against mouse melanoma. J. Immunol., 2011-08-10;187(6):2905-14. 2011-08-10 [PMID: 21832164] (Western Blot, Mouse)
• Ji A, Meyer JM, Cai L et al. Scavenger receptor SR-BI in macrophage lipid metabolism. Atherosclerosis. 4/8/2011 [PMID: 21481393]
• Guimaraes EL, Empsen C, Geerts A, van Grunsven LA Advanced glycation end products induce production of reactive oxygen species via the activation of NADPH oxidase in murine hepatic stellate cells. J. Hepatol., 2009-12-24;52(3):389-97. 2009-12-24 [PMID: 20133001] (ICC, Western Blot, Mouse)
• Yi H, Yu X, Gao P, Wang Y, Baek SH, Chen X, Kim HL, Subjeck JR, Wang XY Pattern recognition scavenger receptor SRA/CD204 down-regulates Toll-like receptor 4 signaling-dependent CD8 T-cell activation. Blood, 2009-04-06;113(23):5819-28. 2009-04-06 [PMID: 19349620] (Western Blot, Mouse)
• Okutsu R, Yoshikawa T, Nagasawa M, Hirose Y, Takase H, Mitani K, Okada K, Miyakoda G, Yabuuchi Y Cilostazol inhibits modified low-density lipoprotein uptake and foam cell formation in mouse peritoneal macrophages. Atherosclerosis, 2008-11-17;204(2):405-11. 2008-11-17 [PMID: 19108834] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: MSR1
• Uniprot:
  • AAA39747()