CD25/IL-2R alpha Antibody (Daclizumab) - Chimeric - Azide and BSA Free

Flow Cytometry: CD25/IL-2R alpha Antibody (Daclizumab) - Chimeric [NBP2-52660] - CD25/IL-2 R alpha Antibody (Daclizumab) [NBP2-52660] - Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Daclizumab (NBP2-52660, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Flow Cytometry: CD25/IL-2R alpha Antibody (Daclizumab) - Chimeric [NBP2-52660] - Cynomolgus monkey lymphocytes were stained with an isotype control (red) or the rabbit-chimeric version of Daclizumab (NBP2-52660, blue) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FlowJo single-cell flow cytometer.

Flow Cytometry: CD25/IL-2R alpha Antibody (Daclizumab) - Chimeric [NBP2-52660] - CD25/IL-2 R alpha Antibody (Daclizumab) [NBP2-52660] - Rhesus monkey lymphocytes were stained with an isotype control (red) or the rabbit-chimeric version of Daclizumab (NBP2-52660, blue) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FlowJo single-cell flow cytometer.

• Reactivity: Hu, Pm-Cm, RM
• Applications: Flow, B/N, CyTOF-ready
• Clone: Daclizumab
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CD25/IL-2R alpha Antibody (Daclizumab) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit CD25/IL-2R alpha Antibody (Daclizumab) - Chimeric - Azide and BSA Free (NBP2-52660) is a recombinant monoclonal antibody validated for use in Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Cultured T-cells derived from a patient with Mycosis fungoides.
• Specificity: Binds to p55 chain of IL2R on human T cells, Tac antigen, which is expressed on T cells activated by mitogens, soluble antigens, and alloantigens but not on resting T cells, thymocytes, B cells, monocytes, activated B cells, leukemic T cell blasts from patients with acute lymphoblastic leukemia, and long-term T cell lines.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IL2RA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • CyTOF-ready
  • Flow Cytometry 1:10 - 1:1000
• Application Notes: This antibody is Cytof ready. This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD25/IL-2R alpha Antibody (Daclizumab) - Chimeric - Azide and BSA Free

• biosimilar antibody
• biosimilars
• CD25 antigen
• CD25
• IDDM10
• IL-2 R alpha
• IL-2 receptor subunit alpha
• IL2R alpha
• IL-2R subunit alpha
• IL2R
• IL2RA
• IL-2Ra
• IL-2-RA
• IL2-RA
• interleukin 2 receptor, alpha
• interleukin-2 receptor subunit alpha
• p55
• TAC antigen
• TCGFR

Background

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.(provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL2RA