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223-2A), Recombinant Mouse CD25/IL-2 R alpha (Catalog # 2438-RM) and Recombinant Rat CD25/IL-2 R alpha Fc Chimera (Catalog # 5156-RM). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CD25/IL-2 R alpha Monoclonal Antibody (Catalog # MAB623) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for CD25/IL-2 R alpha at approximately 30-250 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox" target="_blank">
NL007) and counterstained with DAPI (blue). Upper panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox" target="_blank">
223-2A) was serially diluted and captured by Mouse Anti-Human CD25/IL-2R alpha Monoclonal Antibody (Catalog # MAB623) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CD25/IL-2R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-223-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
HAF017). A specific band was detected for CD25/IL-2R alpha at approximately 60 kDa (as indicated). GAPDH (AF5718) is shown as a loading control.This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
HAF017). A specific band was detected for CD25/IL-2R alpha at approximately 60 kDa (as indicated) in the parental HDLM‑2 human Hodgkin’s lymphoma cell line cell line, but is not detectable in knockout HDLM‑2 human Hodgkin’s lymphoma cell line cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox" target="_blank">
202-IL) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CD25/IL-2 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-223-NA). The ND50 is typically 0.2-1 µg/mL." class="big_lightbox" target="_blank">
223-2A) was serially diluted and captured by Mouse Anti-Human CD25/IL-2R alpha Monoclonal Antibody (Catalog # MAB623) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CD25/IL-2R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-223-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
202-IL) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human CD25/IL-2R alpha His-tag Avi-tag (Catalog # AVI10305) binds with an ED50 of 45.0-540 ng/mL." class="big_lightbox" target="_blank">
Species: Hu Applications: Binding Activity, Bioactivity
The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.(provided by RefSeq)
![Western Blot CD25/IL-2R alpha Antibody (24204) [Unconjugated]](http://images.novusbio.com/images/antibody/CD25_MAB623_Western_Blot_21513.jpg)
![Western Blot CD25/IL-2R alpha Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/CD25_AF223NA_Western_Blot_23992.jpg)
![N/A CD25/IL-2R alpha [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_CD25_DR2A00_ELISA_800.jpg)
![N/A CD25/IL-2R alpha [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_CD25_DY2438_ELISA_1829.jpg)
![N/A CD25/IL-2R alpha [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_CD25_DY223_ELISA_1792.jpg)
![Binding Activity CD25/IL-2R alpha [Biotin]](http://images.novusbio.com/images/avi10305_recombinant-human-cd25-il-2r-alpha-his-avi-tag-protein-cf-binding-activity-2652021223038.jpg)
![Bioactivity CD25/IL-2R alpha [Unconjugated]](http://images.novusbio.com/fullsize/protein/CD25_10305RL_2980.jpg)
![Immunocytochemistry CD25/IL-2R alpha Antibody (24204) [Unconjugated]](http://images.novusbio.com/images/antibody/IL-2_R_alpha_MAB623_Immunocytochemistry_9699.jpg)
![Immunohistochemistry CD25/IL-2R alpha Antibody (24204) [Unconjugated]](http://images.novusbio.com/images/antibody/IL-2_R_alpha_MAB623_Immunohistochemistry_7042.jpg)
![Western Blot CD25/IL-2R alpha Antibody (24204) [Unconjugated]](http://images.novusbio.com/fullsize/mab623_human-cd25-il-2-r-alpha-mab-clone-24204-western-blot-12122025811821.jpg)
![ELISA CD25/IL-2R alpha Antibody (24204) [Unconjugated]](http://images.novusbio.com/images/mab623_human-cd25-il-2r-alpha-mab-clone-24204-elisa-639201776265758075.jpg)
![Knockout Validated CD25/IL-2R alpha Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/af-223-na_human-cd25-il-2-r-alpha-affinity-purified-polyclonal-ab-knockout-validated-2862021151259.jpg)
![Neutralization CD25/IL-2R alpha Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IL-2_R_alpha_AF-223-NA_Block_Neutralize_8657.jpg)
![ELISA CD25/IL-2R alpha Antibody [Unconjugated]](http://images.novusbio.com/images/af-223-na_human-cd25-il-2r-alpha-affinity-purified-polyclonal-ab-elisa-639201850090648158.jpg)
![N/A CD25/IL-2R alpha [HRP]](http://images.novusbio.com/fullsize/elisa/IL-2_R_alpha_DR2A00_ELISA_167.jpg)
![SDS-Page CD25/IL-2R alpha [Biotin]](http://images.novusbio.com/fullsize/avi10305_recombinant-human-cd25-il-2r-alpha-his-avi-tag-protein-cf-sds-page-3132021112416.jpg)
![SDS-Page CD25/IL-2R alpha [Unconjugated]](http://images.novusbio.com/fullsize/protein/CD25_10305RL_2828.jpg)
