CD25/IL-2R alpha Antibody (24212) [Unconjugated]

In a functional flow cytometry test, Recombinant Human IL-2 Biotinylated protein (Catalog # BT202, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human CD25/IL-2R alpha (black dotted line). Binding is completely blocked (orange histogram) by 0.5 μg/mL of Mouse Anti-Human CD25/IL-2R alpha Monoclonal Antibody (Catalog # MAB1020). Mouse IgG2A Isotype Control (Catalog # MAB003) at 0.5 μg/mL was used as a control (blue line). Ligand binding was detected by staining cells with APC-conjugated Streptavidin (Catalog # F0050).

• Reactivity: Hu
• Applications: WB, Flow, B/N, CyTOF-ready
• Clone: 24212
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CD25/IL-2R alpha Antibody (24212) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha
  Glu22-Cys213
  Accession # P01589
• Specificity: Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R gamma or rhIL‑15 R alpha is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL2RA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction 0.5 ug/10^6 cells
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD25/IL-2R alpha Antibody (24212) [Unconjugated]

• CD25 antigen
• CD25
• IDDM10
• IL-2 R alpha
• IL-2 receptor subunit alpha
• IL2R alpha
• IL-2R subunit alpha
• IL2R
• IL2RA
• IL-2Ra
• IL-2-RA
• IL2-RA
• interleukin 2 receptor, alpha
• interleukin-2 receptor subunit alpha
• p55
• TAC antigen
• TCGFR

Background

IL-2 R alpha is a type I transmembrane protein that associates with IL-2 R beta and the common gamma chain to form a high affinity heterotrimeric receptor complex for IL-2. A soluble form of IL-2 R alpha has been detected in serum.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD25/IL-2R alpha Antibody (MAB1020)(5)

Publications using MAB1020

• S Shankar, J Stolp, SC Juvet, J Beckett, PS Macklin, F Issa, J Hester, KJ Wood Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis Nature Communications, 2022-06-03;13(1):3121. 2022-06-03 [PMID: 35660734] (Neutralization, Human)
• McEwen A, Maher M, Mo R, Gottardi C E-cadherin phosphorylation occurs during its biosynthesis to promote its cell surface stability and adhesion. Mol Biol Cell, 2014-06-25;25(16):2365-74. 2014-06-25 [PMID: 24966173] (ICC, Canine)
• Ndhlovu LC, Leal FE, Eccles-James IG, Jha AR, Lanteri M, Norris PJ, Barbour JD, Wachter DJ, Andersson J, Tasken K, Torheim EA, Aandahl EM, Kallas EG, Nixon DF A novel human CD4+ T-cell inducer subset with potent immunostimulatory properties. Eur. J. Immunol., 2010-01-01;40(1):134-41. 2010-01-01 [PMID: 19877008] (Flow Cytometry, Human)
• Ebert EC, Mehta V Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription. Cell. Immunol., 2006-08-17;241(1):7-13. 2006-08-17 [PMID: 16914126] (Flow Cytometry, Human)
• Voo KS, Peng G, Guo Z, Fu T, Li Y, Frappier L, Wang RF Functional characterization of EBV-encoded nuclear antigen 1-specific CD4+ helper and regulatory T cells elicited by in vitro peptide stimulation. Cancer Res., 2005-02-15;65(4):1577-86. 2005-02-15 [PMID: 15735048] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IL2RA
• Uniprot:
  • P01589()