CD25/IL-2R alpha Antibody (24212) [Allophycocyanin]

Human peripheral blood mononuclear cells (PBMCs) treated with 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) and 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B) for 2 days were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Mouse Anti-Human CD25/IL-2 R alpha APC-conjugated Monoclonal Antibody (Catalog # FAB1020A) or (B) Mouse IgG2AAllophycocyanin Isotype Control (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 24212
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

CD25/IL-2R alpha Antibody (24212) [Allophycocyanin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha
  Glu22-Cys213
  Accession # P01589
• Specificity: Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R gamma or rhIL‑15 R alpha is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL2RA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD25/IL-2R alpha Antibody (24212) [Allophycocyanin]

• CD25 antigen
• CD25
• IDDM10
• IL-2 R alpha
• IL-2 receptor subunit alpha
• IL2R alpha
• IL-2R subunit alpha
• IL2R
• IL2RA
• IL-2Ra
• IL-2-RA
• IL2-RA
• interleukin 2 receptor, alpha
• interleukin-2 receptor subunit alpha
• p55
• TAC antigen
• TCGFR

Background

Human CD25, also known as IL-2 receptor alpha (IL-2 R alpha ) and asTac antigen, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2. The IL-2 R alpha cDNA encodes a 272 amino acid residue precursor Type I membrane protein with a 21 residue signal peptide, a 219 residue extracellular region, a 19 residue transmembrane region and a 13 residue cytoplasmic domain. IL-2 R alpha lacks structural features characteristic of members of the cytokine receptor superfamily. By itself, IL-2 R alpha binds IL-2 with low affinity. However, when IL-2 R alpha is associated with the IL-2 receptor beta and gamma chains, a high affinity heterotrimeric receptor complex that transduces IL-2 signals is formed. Soluble forms of many cytokine receptors have been reported, and a soluble form of IL-2 R alpha (IL-2 sR alpha ) appears in serum, concomitant with its increased expression on cells. The function of the soluble IL-2 R alpha is unclear. Increased levels of IL-2 sR alpha in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Increased serum concentration of IL-2 sR alpha has been observed in patients with a variety of inflammatory conditions and in the course of some leukemias and lymphomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD25/IL-2R alpha Antibody (FAB1020A)(4)

Publications using FAB1020A

• Mona A Eldosoky, Reham Hammad, Areej Rushdi, Hanan F Ibrahim, Amany M Tawfeik, Ahmed Mora et al. MicroRNA-146a-5p and microRNA‐210‐3p Correlate with T Regulatory Cells Frequency and Predict Asthma Severity in Egyptian Pediatric Population Journal of Asthma and Allergy 2023-01-20 [PMID: 36714048]
• Qingqu Guo, Yiming Chu, Hongbo Li, Dike Shi, Lele Lin, Weifeng Lan et al. Dickkopf-related protein 3 alters aerobic glycolysis in pancreatic cancer BxPC-3 cells, promoting CD4+ T-cell activation and function European Journal of Medical Research 2021-12-01 [PMID: 34391478]
• Z Lu, N Xu, X Zhou, G Gao, L Li, J Huang, Y Li, Q Lu, B He, C Pan, X Liu Therapeutic immune monitoring of CD4(+)CD25(+) T cells in chronic myeloid leukemia patients treated with tyrosine kinase inhibitors Oncol Lett, 2017-06-01;14(2):1363-1372. 2017-06-01 [PMID: 28808483] (Flow Cytometry, Human)
• M Lecendreux, G Churlaud, F Pitoiset, A Regnault, TA Tran, R Liblau, D Klatzmann, M Rosenzwajg Narcolepsy Type 1 Is Associated with a Systemic Increase and Activation of Regulatory T Cells and with a Systemic Activation of Global T Cells PLoS ONE, 2017-01-20;12(1):e0169836. 2017-01-20 [PMID: 28107375] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IL2RA
• Uniprot:
  • P01589()