CD25/IL-2R alpha Antibody (24212) [Phycoerythrin]

Human peripheral blood mononuclear cells (PBMCs) treated with 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) and 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B) for 2 days were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human CD25/IL-2 R alpha PE-conjugated Monoclonal Antibody (Catalog # FAB1020P) or (B) Mouse IgG2APhycoerythrin Isotype Control (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 24212
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

CD25/IL-2R alpha Antibody (24212) [Phycoerythrin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha
  Glu22-Cys213
  Accession # P01589
• Specificity: Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R gamma or rhIL‑15 R alpha is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL2RA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD25/IL-2R alpha Antibody (24212) [Phycoerythrin]

• CD25 antigen
• CD25
• IDDM10
• IL-2 R alpha
• IL-2 receptor subunit alpha
• IL2R alpha
• IL-2R subunit alpha
• IL2R
• IL2RA
• IL-2Ra
• IL-2-RA
• IL2-RA
• interleukin 2 receptor, alpha
• interleukin-2 receptor subunit alpha
• p55
• TAC antigen
• TCGFR

Background

Human CD25, also known as IL-2 receptor alpha (IL-2 R alpha ) and asTac antigen, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2. The IL-2 R alpha cDNA encodes a 272 amino acid residue precursor Type I membrane protein with a 21 residue signal peptide, a 219 residue extracellular region, a 19 residue transmembrane region and a 13 residue cytoplasmic domain. IL-2 R alpha lacks structural features characteristic of members of the cytokine receptor superfamily. By itself, IL-2 R alpha binds IL-2 with low affinity. However, when IL-2 R alpha is associated with the IL-2 receptor beta and gamma chains, a high affinity heterotrimeric receptor complex that transduces IL-2 signals is formed. Soluble forms of many cytokine receptors have been reported, and a soluble form of IL-2 R alpha (IL-2 sR alpha ) appears in serum, concomitant with its increased expression on cells. The function of the soluble IL-2 R alpha is unclear. Increased levels of IL-2 sR alpha in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Increased serum concentration of IL-2 sR alpha has been observed in patients with a variety of inflammatory conditions and in the course of some leukemias and lymphomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD25/IL-2R alpha Antibody (FAB1020P)(7)

Publications using FAB1020P

• S Shankar, J Stolp, SC Juvet, J Beckett, PS Macklin, F Issa, J Hester, KJ Wood Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis Nature Communications, 2022-06-03;13(1):3121. 2022-06-03 [PMID: 35660734] (Flow Cytometry, Human)
• I Peluso, A Raguzzini, G Catasta, V Cammisotto, A Perrone, C Tomino, E Toti, M Serafini Effects of High Consumption of Vegetables on Clinical, Immunological, and Antioxidant Markers in Subjects at Risk of Cardiovascular Diseases Oxid Med Cell Longev, 2018-10-08;2018(0):5417165. 2018-10-08 [PMID: 30402206] (Flow Cytometry, Human)
• Arneja A, Johnson H, Gabrovsek L, Lauffenburger D, White F Qualitatively different T cell phenotypic responses to IL-2 versus IL-15 are unified by identical dependences on receptor signal strength and duration. J Immunol, 2013-12-02;192(1):123-35. 2013-12-02 [PMID: 24298013] (Flow Cytometry, Human)
• Giuliani M, Fleury M, Vernochet A, Ketroussi F, Clay D, Azzarone B, Lataillade JJ, Durrbach A Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation. 2011-05-19;6(5):e19988. 2011-05-19 [PMID: 21625521] (Flow Cytometry, Human)
• Suzuki H, Onishi H, Wada J, Yamasaki A, Tanaka H, Nakano K, Morisaki T, Katano M VEGFR2 is selectively expressed by FOXP3high CD4+ Treg. Eur. J. Immunol., 2010-01-01;40(1):197-203. 2010-01-01 [PMID: 19902430] (Flow Cytometry, Human)
• Ni Z, Walcheck B Cutaneous lymphocyte-associated antigen (CLA) T cells up-regulate P-selectin ligand expression upon their activation. Clin. Immunol., 2009-08-08;133(2):257-64. 2009-08-08 [PMID: 19665434] (Flow Cytometry, Human)
• Ogbomo H, Michaelis M, Kreuter J, Doerr HW, Cinatl J Histone deacetylase inhibitors suppress natural killer cell cytolytic activity. FEBS Lett., 2007-03-01;581(7):1317-22. 2007-03-01 [PMID: 17349632] (Flow Cytometry, Human)

Review for CD25/IL-2R alpha Antibody (FAB1020P) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 01/31/2019

Summary

• Application: Flow Cytometry
• Sample Tested: CD8 T lymphocytes
• Species: Human
• Lot: LLB0518052

Bioinformatics

• Gene Symbol: IL2RA
• Uniprot:
  • P01589()