CD25/IL-2 R alpha Antibody (AP-MAB0710)

Images

 
Immunohistochemistry-Paraffin: CD25/IL-2 R alpha Antibody (AP-MAB0710) [NB110-97867] - Formalin fixed and paraffin embedded mouse spleen tissue section was subjected to immunohistochemistry staining of CD25.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, IHC-P, CyTOF-ready
Clone
AP-MAB0710
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

CD25/IL-2 R alpha Antibody (AP-MAB0710) Summary

Immunogen
This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with recombinant mouse CD25 protein.
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
IL2RA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:20-1:300
  • CyTOF-ready

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
A 0.2 um filtered solution in PBS
Preservative
No Preservative
Purity
Protein G purified
Reconstitution Instructions
Reconstitute with 0.4 ml sterilized PBS to a final concentration of 0.5 mg/ml.

Alternate Names for CD25/IL-2 R alpha Antibody (AP-MAB0710)

  • CD25 antigen
  • CD25
  • IDDM10
  • IL-2 R alpha
  • IL-2 receptor subunit alpha
  • IL2R alpha
  • IL-2R subunit alpha
  • IL2R
  • IL2RA
  • IL-2Ra
  • IL-2-RA
  • IL2-RA
  • interleukin 2 receptor, alpha
  • interleukin-2 receptor subunit alpha
  • p55
  • TAC antigen
  • TCGFR

Background

The interleukin 2 (IL2) receptor alpha (IL2RA) also called CD25 and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low affinity receptor, while homodimeric beta (IL2RB) chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for CD25/IL-2 R alpha Antibody (NB110-97867). (Showing 1 - 1 of 1 FAQ).

  1. I was wondering how you ran this antibody in paraffin tissue?
    • This product was used in IHC-P using our standard IHC protocol found here: Protocol. The antigen retrieval method employed was Proteinase K (20ug/ml in PBS) incubated on the section for 15 min at room temperature.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-97867AF405
Alexa Fluor 488 Labeled NB110-97867AF488
Alexa Fluor 647 Labeled NB110-97867AF647
Alexa Fluor 700 Labeled NB110-97867AF700
Allophycocyanin Labeled NB110-97867APC
DyLight 350 Labeled NB110-97867UV
DyLight 405 Labeled NB110-97867V
DyLight 488 Labeled NB110-97867G
DyLight 680 Labeled NB110-97867FR
DyLight 755 Labeled NB110-97867IR
PE Labeled NB110-97867PE
PerCP Labeled NB110-97867PCP

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Bioinformatics

Gene Symbol IL2RA
Entrez
Uniprot