CD3 Antibody (17A2) [Unconjugated]

Images

 
CD3 was detected in perfusion fixed HSV-1 infected frozen sections of mouse brain (perivascular cuffing) using 10 µg/mL Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) overnight at 4 °C. Tissue was stained ...read more
CD3 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, IP, CyTOF-ready, Func, ICC/IF
Clone
17A2
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

CD3 Antibody (17A2) [Unconjugated] Summary

Immunogen
T cell hybridoma D1
Specificity
Reacts with mouse TCR-associated CD3 complex that occurs on thymocytes and mature T cells.
Details of Functionality
ActivityAssaywCitation not found None None None None None None
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
Cd247
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation
  • Complement-dependent Cytotoxicity
  • CyTOF-ready
  • Immunocytochemistry 3-25 ug/mL
  • T Cell Stimulation 1 - 5 ug/mL
Publications
Read Publications using
MAB4841 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD3 Antibody (17A2) [Unconjugated]

  • CD3 antigen, delta subunit
  • CD3
  • CD3d antigen
  • CD3d antigen, delta polypeptide (TiT3 complex)
  • CD3d molecule, delta (CD3-TCR complex)
  • CD3-DELTA
  • CD3e antigen
  • CD3e antigen, epsilon polypeptide (TiT3 complex)
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3-epsilon
  • CD3g antigen
  • CD3g antigen, gamma polypeptide (TiT3 complex)
  • CD3g molecule, epsilon (CD3-TCR complex)
  • CD3g molecule, gamma (CD3-TCR complex)
  • CD3G
  • CD3-GAMMA
  • FLJ17620
  • FLJ17664
  • FLJ18683
  • FLJ79544
  • FLJ94613
  • MGC138597
  • T3DOKT3, delta chain
  • T3E
  • T-cell antigen receptor complex, epsilon subunit of T3
  • T-cell receptor T3 delta chain
  • T-cell surface antigen T3/Leu-4 epsilon chain
  • T-cell surface glycoprotein CD3 delta chain
  • T-cell surface glycoprotein CD3 epsilon chain
  • TCRE

Background

The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD3 Antibody (MAB4841)(19)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: Activation, Bioassay, Functional Assay, ICC, IHC, IHC-P.


Filter By Application
Activation
(1)
Bioassay
(1)
Functional Assay
(8)
ICC
(1)
IHC
(6)
IHC-P
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(17)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using MAB4841 Applications Species
J Wu, J Gu, S Zhou, H Lu, Y Lu, L Lu, X Wang Anti-IL-22 Antibody Attenuates Acute Graft-versus-Host Disease via Increasing Foxp3+ T Cell through Modulation of CD11b+ Cell Function J Immunol Res, 2018;2018(0):1605341. 2018 [PMID: 30159338] (Activation, Mouse) Activation Mouse
MR Deyhle, PS Hafen, J Parmley, CN Preece, M Robison, JR Sorensen, B Jackson, DL Eggett, CR Hancock, RD Hyldahl CXCL10 increases in human skeletal muscle following damage but is not necessary for muscle regeneration Physiol Rep, 2018;6(8):e13689. 2018 [PMID: 29696819] (IHC, Mouse) IHC Mouse
P Chauhan, WS Sheng, S Hu, S Prasad, JR Lokensgard Nitrosative damage during retrovirus infection-induced neuropathic pain J Neuroinflammation, 2018;15(1):66. 2018 [PMID: 29506535] (IHC, Mouse) IHC Mouse
S Swirski, C Röger, A Pienkowska, C Ihlenburg, G Fischer, O May, M Vorm, M Owczarek-L, J Neidhardt A Novel C-Terminal Mutation in Gsdma3 (C+/H-) Leads to Alopecia and Corneal Inflammatory Response in Mice Invest. Ophthalmol. Vis. Sci., 2018;59(1):561-571. 2018 [PMID: 29372254] (IHC, Mouse) IHC Mouse
W Lei, D Zeng, G Liu, Y Zhu, J Wang, H Wu, J Jiang, J Huang Crucial role of OX40/OX40L signaling in a murine model of asthma Mol Med Rep, 2018;0(0):. 2018 [PMID: 29344664] (Functional Assay, Mouse) Functional Assay Mouse
X Fu, F Sun, F Wang, J Zhang, B Zheng, J Zhong, T Yue, X Zheng, JF Xu, CY Wang Aloperine Protects Mice against DSS-Induced Colitis by PP2A-Mediated PI3K/Akt/mTOR Signaling Suppression Mediators Inflamm., 2017;2017(0):5706152. 2017 [PMID: 29056830] (Functional Assay, Mouse) Functional Assay Mouse
R Fischer, J Marsal, C Guttà, SA Eisler, N Peters, JR Bethea, K Pfizenmaie, RE Kontermann Novel strategies to mimic transmembrane tumor necrosis factor-dependent activation of tumor necrosis factor receptor 2 Sci Rep, 2017;7(1):6607. 2017 [PMID: 28747780] (Functional Assay, Human) Functional Assay Human
W Pan, WT Hao, YJ Shen, XY Li, YJ Wang, FF Sun, JH Yin, J Zhang, RX Tang, JP Cao, KY Zheng The excretory-secretory products of Echinococcus granulosus protoscoleces directly regulate the differentiation of B10, B17 and Th17 cells Parasit Vectors, 2017;10(1):348. 2017 [PMID: 28732522] (Functional Assay, Mouse) Functional Assay Mouse
PG Makker, SS Duffy, JG Lees, CJ Perera, RS Tonkin, O Butovsky, SB Park, D Goldstein, G Moalem-Tay Characterisation of Immune and Neuroinflammatory Changes Associated with Chemotherapy-Induced Peripheral Neuropathy PLoS ONE, 2017;12(1):e0170814. 2017 [PMID: 28125674] (IHC, Mouse) IHC Mouse
Selective suppression of the ? isoform of p38 MAPK rescues late-stage tau pathology Alzheimers Res Ther, 2016;8(1):54. 2016 [PMID: 27974048] (ICC, Mouse) ICC Mouse
Show All 19 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD3 Antibody (MAB4841). (Showing 1 - 1 of 1 FAQs).

  1. What the theoretical molecular weight for CD3 antibodies?
    • The TMW of CD3 antibodies is approximately 20 - 25 kDa.

Secondary Antibodies

 

Isotype Controls

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Blogs on CD3.

Transferrin and the blood brain barrier
Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

CD3 (OKT3) as a marker of immune response efficiency
Our immune system is a powerful defense mechanism against infection, however different variables can cause our immune response to work for or against us.  CD3 (cluster of differentiation 3) is one component of our immune signal response that is co...  Read full blog post.

The CD4 Antibody: More than Just a Cellular Marker
CD4 is a member of the cluster of differentiation family of proteins, mainly expressed on the surface of thymocytes and a specific subset of mature T-cells. CD4 antibody studies have also shown it expressed on monocytes, cortical cells, microglial cel...  Read full blog post.

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Bioinformatics

Gene Symbol Cd247