CD69 Antibody [Unconjugated]

Mouse splenocytes treated with 50ng/mL PMA and 200ng/mL Ionomycin overnight (A) vs unstimulated mouse splenocytes (B) were stained with Goat Anti-Mouse CD69 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2386) and Rat Anti-Mouse CD3 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4841F) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CD69 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD69
  Gly64-Arg199
  Accession # P37217
• Specificity: Detects mouse CD69 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross‑reactivity with recombinant human CD69 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CD69
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD69 Antibody [Unconjugated]

• activation inducer molecule (AIM/CD69)
• Activation inducer molecule
• AIM
• BL-AC/P26
• CD69 antigen (p60, early T-cell activation antigen)
• CD69 antigen
• CD69 molecule
• CD69
• CLEC2CC-type lectin domain family 2 member C
• C-type lectin domain family 2, member C
• EA1
• EA-1
• early activation antigen CD69
• early lymphocyte activation antigen
• Early T-cell activation antigen p60
• GP32/28
• Leu23
• Leukocyte surface antigen Leu-23
• MLR-3
• p60

Background

CD69, also known as Leu 23, AIM, EA-1 and MLR-3, is a type II transmembrane glycoprotein and is expressed on activated T cells, B cells, NK cells, neutrophils and eosinophils, Langerhans cells and platelets. The C-terminal extracellular domain of mouse CD69 shares approximately 57% amino acid sequence homology with the corresponding region of human CD69.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD69 Antibody (AF2386)(5)

Publications using AF2386

• T Kaya, N Mattugini, L Liu, H Ji, L Cantuti-Ca, J Wu, M Schifferer, J Groh, R Martini, S Besson-Gir, S Kaji, A Liesz, O Gokce, M Simons CD8+ T cells induce interferon-responsive oligodendrocytes and microglia in white matter aging Nature Neuroscience, 2022-10-24;25(11):1446-1457. 2022-10-24 [PMID: 36280798] (IHC, Mouse)
• C Wagner, J Bonnardel, C Da Silva, L Spinelli, CA Portilla, J Tomas, M Lagier, L Chasson, M Masse, M Dalod, A Chollat-Na, JP Gorvel, H Lelouard Differentiation Paths of Peyer's Patch LysoDCs Are Linked to Sampling Site Positioning, Migration, and T Cell Priming Cell Rep, 2020-04-07;31(1):107479. 2020-04-07 [PMID: 32268097] (ICC, Human)
• Glennys V. Reynoso, Andrea S. Weisberg, John P. Shannon, Daniel T. McManus, Lucas Shores, Jeffrey L. Americo et al. Lymph node conduits transport virions for rapid T cell activation Nature Immunology 5/1/2019 [PMID: 30886418]
• U Jadhav, M Saxena, NK O'Neill, A Saadatpour, GC Yuan, Z Herbert, K Murata, RA Shivdasani Dynamic Reorganization of Chromatin Accessibility Signatures during Dedifferentiation of Secretory Precursors into Lgr5+ Intestinal Stem Cells Cell Stem Cell, 2017-06-22;0(0):. 2017-06-22 [PMID: 28648363] (IHC, Mouse)
• Hickman H, Reynoso G, Ngudiankama B, Cush S, Gibbs J, Bennink J, Yewdell J CXCR3 chemokine receptor enables local CD8(+) T cell migration for the destruction of virus-infected cells. Immunity, 2015-03-10;42(3):524-37. 2015-03-10 [PMID: 25769612] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: CD69
• Uniprot:
  • P37217()