CD25/IL-2R alpha Antibody (AP-MAB0710) - Azide and BSA Free

Immunohistochemistry-Paraffin: CD25/IL-2 R alpha Antibody (AP-MAB0710) [NB110-97867] - Formalin fixed and paraffin embedded mouse spleen tissue section was subjected to immunohistochemistry staining of CD25.

• Reactivity: Mu, Rt
• Applications: Flow, IHC, CyTOF-ready
• Clone: AP-MAB0710
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

CD25/IL-2R alpha Antibody (AP-MAB0710) - Azide and BSA Free Summary

• Immunogen: Mouse recombinant CD25/IL-2R alpha
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: IL2RA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:20-1:300
• Application Notes: This antibody is Cytof ready.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 30134210).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Alternate Names for CD25/IL-2R alpha Antibody (AP-MAB0710) - Azide and BSA Free

• CD25 antigen
• CD25
• IDDM10
• IL-2 R alpha
• IL-2 receptor subunit alpha
• IL2R alpha
• IL-2R subunit alpha
• IL2R
• IL2RA
• IL-2Ra
• IL-2-RA
• IL2-RA
• interleukin 2 receptor, alpha
• interleukin-2 receptor subunit alpha
• p55
• TAC antigen
• TCGFR

Background

The interleukin 2 (IL2) receptor alpha (IL2RA) also called CD25 and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low affinity receptor, while homodimeric beta (IL2RB) chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD25/IL-2R alpha Antibody (NB110-97867)(2)

Publications using NB110-97867

• Izadi Z, Hajizadeh-Saffar E, Hadjati J et al. Tolerance induction by surface immobilization of Jagged-1 for immunoprotection of pancreatic islets Biomaterials Aug 10 2018 [PMID: 30134210] (Flow, Rat) Biomaterials. 2018-11-01 [PMID: 30134210] (FLOW, Rat)
  
  Details:
  Citation using the PE form of this antibody.
• Kim So Young, Kang Dongxu, Choi Hye Jin et al. Prime-boost immunization by both DNA vaccine and oncolytic adenovirus expressing GM-CSF and shRNA of TGF-b2 induces anti-tumor immune activation. Oncotarget 2017-01-01 [PMID: 28178658] (IF/IHC, Mouse)

FAQs for CD25/IL-2R alpha Antibody (NB110-97867). (Showing 1 - 1 of 1 FAQs).

1. I was wondering how you ran this antibody in paraffin tissue?
   • This product was used in IHC-P using our standard IHC protocol found here: Protocol. The antigen retrieval method employed was Proteinase K (20ug/ml in PBS) incubated on the section for 15 min at room temperature.

Bioinformatics

• Gene Symbol: IL2RA
• Entrez:
  • 16184(Mouse)
• Uniprot:
  • P01590()