CD21 Antibody (BB6-11C9.6) [Biotin]

Flow Cytometry: CD21 Antibody (BB6-11C9.6) [Biotin] [NBP1-28247] - Porcine peripheral blood lymphocytes were stained with Mouse Anti-Porcine CD21-BIOT and Mouse Anti-Porcine CD3 epsilon-PE followed by Streptavidin-FITC.

• Reactivity: Mu, Po
• Applications: Flow
• Clone: BB6-11C9.6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin

CD21 Antibody (BB6-11C9.6) [Biotin] Summary

• Immunogen: Porcine CD21, Mr 160 kDa
• Marker: Histiocytic Marker
• Specificity: Porcine CD21, Mr 160 kDa
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CR2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26284585). Use in Mouse reported in scientific literature (PMID:26284585).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for CD21 Antibody (BB6-11C9.6) [Biotin]

• C3DR
• C3DRSLEB9
• CD21 antigen
• CD21
• Complement C3d receptor
• complement component (3d/Epstein Barr virus) receptor 2
• complement receptor type 2
• CR2
• EBV receptor
• Epstein-Barr virus receptor

Background

CD21, also known as complement receptor 2, is expressed on all mature circulating B lymphocytes but not on T lymphocytes or natural killer cells. (1) Low density CD21 has also been reported on granulocytes and monocytes. MAb BB6-11C9. (6) stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 92-96% in peripheral blood; 74-98% in mesenteric lymph nodes; 97-100% in ileal Peyer's patches (ILPP); 93-97% in spleen; and 0% in thymus. (2) The antibody does not stain B cells in cord blood or spleen from pig fetuses (105 days gestation). (2)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD21 Antibody (NBP1-28247)(1)

Publication using NBP1-28247

• Ramm R, Goecke T, KOhler P Et al. Immunological and functional features of decellularized xenogeneic heart valves after transplantation into GGTA1-KO pigs Regen Biomater 2022-08-05 [PMID: 35928180]
  
  Details:
  Citation using the Biotin version of this antibody.

Bioinformatics

• Gene Symbol: CR2