CD19 Antibody (HIB19)

Images

 
Flow Cytometry: CD19 Antibody (HIB19) [NBP1-43436] - Analysis using the PerCP/Cy5.5 conjugate of NBP1-43436. Staining of normal human peripheral blood cells with Anti-Human CD3 FITC and staining buffer ...read more
Flow Cytometry: CD19 Antibody (HIB19) [NBP1-43436] - Staining of normal human peripheral blood cells with Anti-Human CD19 FITC (left) and PE (right). Autofluorescence is indicated by open histogram. Cells in the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, IHC-Fr, In vitro
Clone
HIB19
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

CD19 Antibody (HIB19) Summary

Immunogen
The immunogen for this antibody was CD19.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD19
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry < 1ug/10^6 cells
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • In vitro assay
Application Notes
The HIB19 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD19 Antibody (HIB19)

  • B4
  • B-lymphocyte antigen CD19
  • B-lymphocyte surface antigen B4
  • CD19 antigen
  • CD19 molecule
  • CD19
  • CVID3
  • Differentiation antigen CD19
  • Leu-12
  • MGC12802
  • T-cell surface antigen Leu-12

Background

The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, Leu13, MHC class II, and CD19 form a multimolecular complex that associates with BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Blogs on CD19.

CD19: An Undoubted Biomarker for B Cells
CD19 is a cell surface protein member of the large immunoglobulin superfamily that complexes with CD21, CD81, and CD225 in the membrane of mature B-cells. A major function of CD19 is to assemble with the antigen receptor of B-lymphocytes to decrease t...  Read full blog post.

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Bioinformatics

Gene Symbol CD19
Entrez