CD163 Antibody (K20-T)

Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148] - CD163 immunolabelling on a FFPE tissue section of human HCV liver biopsy. Image provided by Victoria Gadd.

Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148] - CD163 expression in the macrophages of the bone marrow. FFPE human tissues (4 um sections) stained with anti-CD163.

Immunocytochemistry/Immunofluorescence: CD163 Antibody (K20-T) [NBP1-30148] - Human primary macrophages in 3D cell culture. Primary antibodies to CD163 (NBP1-30148; RED) and CD206 (clone 15-2; GREEN) were both diluted at 1:100 in blocking buffer and incubated overnight at 4C. This image represents a 100-micron Z-Stack displayed as maximum intensity projection. Image from verified customer review.

Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148] - Formalin fixed, paraffin embedded human tissues (4 um sections) stained with CD163 Antibody (K20-T).

Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148] - Human bone marrow .

Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148] - CD163-positive macrophages in the abscess of the liver. FFPE human tissues (4 um sections) stained with anti-CD163

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clone: K20-T
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CD163 Antibody (K20-T) Summary

• Description: This antibody is immunoaffinity purified with immunogenic peptide as a ligand.
• Immunogen: Peptide derived from N-terminal sequence of human CD163. Antibody recognizes the epitope between Gly134 - Gly148.
• Epitope: Gly134 - Gly148
• Marker: Histiocytic Marker
• Specificity: This antibody is specific for the N terminus of CD163.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CD163
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100 -1:200
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100 - 1:200
• Application Notes: Use in Immunohistochemistry-Frozen was reported in scientific literature (PMID: 24798518).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 20mM Tris-HCl (pH 8.0) and 20mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Alternate Names for CD163 Antibody (K20-T)

• CD_antigen: CD163
• CD163 molecule
• CD163
• GHI/61
• HbSR
• Hemoglobin scavenger receptor
• M130
• macrophage-associated antigen
• MM130
• RM3/1
• SCARI1
• scavenger receptor cysteine-rich type 1 protein M130
• sCD163
• Soluble CD163

Background

CD163 (Cluster of Differentiation 163), also known by several other names including M130, p155, RM3/1, Ki-M8, Ber-MAC3, SM4, and GHI/61, is a type I transmembrane glycoprotein that is a member of the scavenger receptor cysteine-rich (SRCR) super family class B (1-3). CD163 is expressed specifically on the monocyte/macrophage lineage and has a theoretical molecular weight of 130-160 kDa in reducing conditions and 110 kDa in non-reducing conditions (2 - 6). CD163 is synthesized as 1076 amino acid (aa) protein consisting of a large extracellular domain (ECD) with nine SRCR domains, proline-serine-threonine rich (PST) linker domain, a transmembrane segment, and a cytoplasmic tail which has varying lengths depending on the isoform due to alternative splicing, with the short 49 aa form being the most common (1-3, 6).

One of the primary functions of CD163 is uptake of haptoglobin-hemoglobin (Hp-Hb) complexes from the liver, spleen, and bone marrow, ultimately triggering an anti-inflammatory response (3, 5, 7). CD163 also functions as an erythroblast adhesion receptor and promotes cell maturation and survival (3, 5, 7). Furthermore, CD163 functions in immune sensing of bacteria and as a receptor for tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) (3, 5, 7). As mentioned above, CD163 is expressed on cells in the monocyte/macrophage lineage and, in general, anti-inflammatory signals including glucocorticoids, interleukin (IL)-6, and IL-10 stimulate CD163 synthesis and expression while, conversely, pro-inflammatory signals such as interferon-gamma (INF-gamma), TNF-alpha, and lipopolysaccharide (LPS) downregulate CD163 (3, 5). In addition to membrane-bound form of CD163, the protein can be cleaved by metalloproteinases (MMP) and induced by LPS or phorbol myristate acetate (PMA) to release a soluble form (sCD163) into the plasma (7). Increased levels of sCD163 in the plasma and an increased number of CD163-expressing macrophages at the site of inflammation are associated with a variety of pathologies (3, 5-7). CD163/sCD163 is often increased and a suitable clinical marker for inflammatory diseases including rheumatoid arthritis (RA), Gaucher disease, chronic kidney disease, diabetes, and Crohn's disease (3, 5-7).

Alternative names for CD163 includes GHI/61, HbSR, Hemoglobin scavenger receptor, M130, macrophage-associated antigen, MM130, RM3/1, SCARI1, scavenger receptor cysteine-rich type 1 protein M130, sCD163, and soluble CD163.

References

1. Law, S. K., Micklem, K. J., Shaw, J. M., Zhang, X. P., Dong, Y., Willis, A. C., & Mason, D. Y. (1993). A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily. European journal of immunology. https://doi.org/10.1002/eji.1830230940

2. Onofre, G., Kolackova, M., Jankovicova, K., & Krejsek, J. (2009). Scavenger receptor CD163 and its biological functions. Acta medica (Hradec Kralove).

3. Van Gorp, H., Delputte, P. L., & Nauwynck, H. J. (2010). Scavenger receptor CD163, a Jack-of-all-trades and potential target for cell-directed therapy. Molecular immunology. https://doi.org/10.1016/j.molimm.2010.02.008

4. Sulahian, T. H., Hogger, P., Wahner, A. E., Wardwell, K., Goulding, N. J., Sorg, C., Droste, A., Stehling, M., Wallace, P. K., Morganelli, P. M., & Guyre, P. M. (2000). Human monocytes express CD163, which is upregulated by IL-10 and identical to p155. Cytokine. https://doi.org/10.1006/cyto.2000.0720

5. Etzerodt, A., & Moestrup, S. K. (2013). CD163 and inflammation: biological, diagnostic, and therapeutic aspects. Antioxidants & redox signaling. https://doi.org/10.1089/ars.2012.4834

6. Skytthe, M. K., Graversen, J. H., & Moestrup, S. K. (2020). Targeting of CD163+ Macrophages in Inflammatory and Malignant Diseases. International journal of molecular sciences, 21(15), 5497. https://doi.org/10.3390/ijms21155497

7. Moller H. J. (2012). Soluble CD163. Scandinavian journal of clinical and laboratory investigation. https://doi.org/10.3109/00365513.2011.626868

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD163 Antibody (NBP1-30148)(9)

Publications using NBP1-30148

• FH Brasil da, MS Lewis, A Truong, DD Carson, MC Farach-Car SULF1 suppresses Wnt3A-driven growth of bone metastatic prostate cancer in perlecan-modified 3D cancer-stroma-macrophage triculture models PLoS ONE, 2020-05-15;15(5):e0230354. 2020-05-15 [PMID: 32413029]
• Tabata T, Petitt M, Fang-Hoover J et al. Neutralizing Monoclonal Antibodies Reduce Human Cytomegalovirus Infection and Spread in Developing Placentas Vaccines (Basel) 2019-09-29 [PMID: 31569508] (ICC/IF, Human)
• Locke LW, Crouser ED, White P et al. IL-13-regulated Macrophage Polarization during Granuloma Formation in an In Vitro Human Sarcoidosis Model Am. J. Respir. Cell Mol. Biol. 2018-08-22 [PMID: 30134122] (ICC/IF, Human)
• Gibier JB, Hemon B, Fanchon M et al. Dual role of MUC1 mucin in kidney ischemia-reperfusion injury: Nephroprotector in early phase, but pro-fibrotic in late phase. Biochim. Biophys. Acta. 2017-03-31 [PMID: 28366875]
• O'Brown ZK, Van Nostrand EL, Higgins JP et al. The Inflammatory Transcription Factors NFkB, STAT1 and STAT3 Drive Age-Associated Transcriptional Changes in the Human Kidney. PLoS Genet. 2015-12-01 [PMID: 26678048]
• Azad AK, Rajaram MV, Metz WL et al. gamma-Tilmanocept, a New Radiopharmaceutical Tracer for Cancer Sentinel Lymph Nodes, Binds to the Mannose Receptor (CD206) J. Immunol. 2015-09-01 [PMID: 26202986] (IHC-P, Human)
• Drouin-Ouellet J, Sawiak SJ, Cisbani G et al. Cerebrovascular and blood-brain barrier impairments in Huntington's disease: Potential implications for its pathophysiology Ann. Neurol. 2015-04-09 [PMID: 25866151] (IHC-P, Human)
• Cicchetti F, Lacroix S, Cisbani G et al. Mutant huntingtin is present in neuronal grafts in Huntington's disease patients. Ann. Neurol. 2014-05-06 [PMID: 24798518] (IHC-Fr, Human)
• Gadd VL, Melino M, Roy S et al. Portal, but not lobular, macrophages express matrix metalloproteinase-9: association with the ductular reaction and fibrosis in chronic hepatitis C Liver Liver Int 2012-11-12 [PMID: 23240894] (ICC/IF, IF/IHC, Human)

Reviews for CD163 Antibody (NBP1-30148) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Fabio Henrique Brasil da Costa ICC Human 11/26/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: 3D Cell culture,Human primary macrophages
• Species: Human
• Lot: DB045-02-0BA

Comments

• Comments: IF of human primary macrophages, polarized on dishes to M2 phenotype for 7 days with M-CSF and IL-4, 10 and 13. Then, cells were harvested and encapsulated into hyaluronic acid and collagen type I hydrogels and cultured for an additional 5 days, on which they were fixed with 4% PFA. Blocked in 10% goat serum, 1% BSA.This data is supported by qRT-PCR analysis of CD163 expression in another group of cells that received the same treatment. All compared to unpolarized control cells.

reviewed by: Verified Customer IHC-P Human 03/04/2013

Summary

• Application: Immunohistochemistry-Paraffin
• Species: Human

FAQs for CD163 Antibody (NBP1-30148). (Showing 1 - 2 of 2 FAQs).

1. Can you please tell me antigen retrieval methods used for this antibody?
   • For this product, our lab recommends HIER with (10 mM Tris/1 mM EDTA pH 9.0 solution). The protocol is detailed below: Pre-heat steamer or water bath with staining dish containing Tris/EDTA solution until temperature reaches 95-100 degrees Celsius. Immerse slides in the staining dish. Place the lid loosely on the staining dish and incubate for 20-40 minutes. Remove the staining dish to room temperature and allow the slides to cool for 20 minutes before proceeding with normal staining procedure.
2. Can you please tell me antigen retrieval methods used for this product (citrate or EDTA) NBP1-30148?
   • For this product, our lab recommends HIER with (10 mM Tris/1 mM EDTA pH 9.0 solution). Protocol is detailed below: Pre-heat steamer or water bath with staining dish containing Tris/EDTA solution until temperature reaches 95-100 degrees Celsius. Immerse slides in the staining dish.  Place the lid loosely on the staining dish and incubate for 20-40 minutes. Remove the staining dish to room temperature and allow the slides to cool for 20 minutes before proceeding with normal staining procedure.

Bioinformatics

• Gene Symbol: CD163
• Entrez:
  • 9332(Human)
• Uniprot:
  • Q86VB7()