Anti-CD163 Antibodies: Novus Biologicals

NB110-40686

CD163 Antibody (EDHu-1) - BSA Free

(4 Reviews)

0.1 mg

NB110-40686

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cytoplasm. Protocol available in COMET™ Panel Builder." class="big_lightbox">

CD163 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human CD163 Monoclonal Antibody (Novus Catalog # NB110-40686) at 1:50 at 37 °Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cytoplasm. Protocol available in COMET™ Panel Builder." alt="CD163 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human CD163 Monoclonal Antibody (Novus Catalog # NB110-40686) at 1:50 at 37 °Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cytoplasm. Protocol available in COMET™ Panel Builder." class="big_thumb" />

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cytoplasm. Protocol available in COMET™ Panel Builder." class="big_lightbox">

CD163 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD163 Monoclonal Antibody (Novus Catalog # NB110-40686) at 20 µg/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 8 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cytoplasm. Protocol available in COMET™ Panel Builder." class="big_thumb" />

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cytoplasm. Protocol available in COMET™ Panel Builder." class="big_lightbox">

CD163 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CD163 Monoclonal Antibody (Novus Catalog # NB110-40686) at 1:50 at 37 °Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane and cytoplasm. Protocol available in COMET™ Panel Builder." class="big_thumb" />

CD163-Antibody-EDHu-1-Immunohistochemistry-NB110-40686-img0013.jpg

A surface stain was performed on hPBMCs with CD163 (EDHu-1) antibody NB110-40686AF700 and a matched isotype control NBP2-27287AF700. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758AF488. Image using the Alexa Fluor 700 form of this antibody.

Staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1.

A surface stain was performed on human peripheral blood monocytes with CD163 (EDHu-1) antibody NB110-40686APC and a matched isotype control NBP2-27287APC. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758AF488.

FFPE human tonsil, stained with anti-CD163, pH 9 antigen retrieval. IHC-P Image submitted by a verified customer review.

A surface stain was performed on hPBMCs with CD163 (EDHu-1) antibody NB110-40686PE and a matched isotype control NBP2-27287PE. Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758APC. Image using the PE form of this antibody.

Human tonsil cryosection with Mouse anti Human CD163 antibody, clone, red in A and Mouse anti Human CD21 antibody, green in B. C is the merged image with nuclei counterstained blue using DAPI.

Frozen human skin sections. CD163 (green) in control human skin. Sections were fixed with 4% PFA PBS, permeabilized wtih 0.5% Triton TBS Ca/Azide, and blocked with a 5% BSA 0.05% T20 TBS Ca/Azide solution. Antibody was diluted 1:100 and applied for 1 hr at 37C. Detection was performed with anti-host conjugated antibody, and sections were counterstained with DAPI. IHC-Fr image submitted by a verified customer review.

Immunohistochemistry-Frozen: CD163 Antibody (EDHu-1) [NB110-40686] - Staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1.

Immunohistochemistry-Paraffin: Mouse Monoclonal CD163 Antibody (EDHu-1) [NB110-40686] - CD163 immunoreactivity in a formalin fixed paraffin embedded section of canine lymph node. NB110-40686 was diluted to 0.25ug per mL and was left on the tissue section for 30m at room temperature. Section underwent 20m of heat induced epitope retrieval in a vegetable steamer using a citrate buffer. No signal was detected in sections that did not undergo HIER. Image from a verified customer review.

Immunohistochemistry-Paraffin: Mouse Monoclonal CD163 Antibody (EDHu-1) [NB110-40686] - CD163 immunoreactivity in an FFPE section of human tonsil. NB110-40686 was diluted to 250 ng/mL and left on tissue sections for 30min at room temperature. Secondary was horse anti mouse HRP polymer. Image from a verified customer review.

Immunohistochemistry-Paraffin: Mouse Monoclonal CD163 Antibody (EDHu-1) [NB110-40686] - CD163 immunoreactivity in an FFPE section of equine spleen. NB110-40686 was diluted to 250ng per mL and left on tissue sections for 30min at room temperature. Secondary was horse anti mouse HRP polymer. Image from a verified customer review.

Immunocytochemistry/ Immunofluorescence: CD163 Antibody (EDHu-1) [NB110-40686] - Non-repaired & repaired VML injured muscles present sustained putative macrophage infiltration. Representative histologic images labeled with CD163 (pan macrophage marker) & wheat germ agglutinin (WGA) & DAPI (nuclei). (a) For each of the sham muscle sections individual & merged images are presented. (b) Examples for each group, specifically the non-, SIS-, UMB-, & Hya-reparied are shown. Scale bar is 150 µm; all images are at the same magnification & follow the same location pattern displayed specifically in Fig. 4e. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29030619), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-40686AF350
Alexa Fluor 405	NB110-40686AF405
Alexa Fluor 488	NB110-40686AF488
Alexa Fluor 532	NB110-40686AF532
Alexa Fluor 594	NB110-40686AF594
Alexa Fluor 647	NB110-40686AF647
Alexa Fluor 700	NB110-40686AF700
Alexa Fluor 750	NB110-40686AF750
Alexa Fluor Plus 405	NB110-40686AFP405
Alexa Fluor Plus 488	NB110-40686AFP488
Alexa Fluor Plus 555	NB110-40686AFP555
Alexa Fluor Plus 594	NB110-40686AFP594
Alexa Fluor Plus 647	NB110-40686AFP647
Alexa Fluor Plus 680	NB110-40686AFP680
Allophycocyanin	NB110-40686APC
Allophycocyanin/Cy7	NB110-40686APCCY7
Biotin	NB110-40686B
CoraFluor 1	NB110-40686CL1
DyLight 350	NB110-40686UV
DyLight 405	NB110-40686V
DyLight 488	NB110-40686G
DyLight 550	NB110-40686R
DyLight 594	NB110-40686DL594
DyLight 650	NB110-40686C
DyLight 680	NB110-40686FR
DyLight 755	NB110-40686IR
FITC	NB110-40686F
HRP	NB110-40686H
Janelia Fluor 525	NB110-40686JF525
Janelia Fluor 549	NB110-40686JF549
Janelia Fluor 646	NB110-40686JF646
Janelia Fluor 669	NB110-40686JF669
PE	NB110-40686PE
PE/Atto594	NB110-40686PEATT594
PE/Cy5.5	NB110-40686PECY55
PE/Cy7	NB110-40686PECY7
PerCP	NB110-40686PCP
mFluor Violet 450 SE	NB110-40686MFV450
mFluor Violet 500 SE	NB110-40686MFV500
mFluor Violet 610 SE	NB110-40686MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-40686-0.025mg

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NB110-59935

CD163 Antibody (10D6)

(1 Review)

(23 Publications)

0.5 ml

NB110-59935

Immunohistochemistry-Paraffin: CD163 Antibody (10D6) [NB110-59935] - Human lymph node stained with CD163 antibody showing cytoplasmic staining in non-germinal center cells. Image from verified customer review.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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AF1607

CD163 Antibody [Unconjugated]

(1 Review)

(16 Publications)

100 μg

AF1607

HAF019). A specific band was detected for CD163 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human thymus tissue, human liver tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD163 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1607) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF019). A specific band was detected for CD163 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human thymus tissue, human liver tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD163 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1607) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for CD163 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for CD163 at approximately 179 kDa (as indicated) using 10 µg/mL of Goat Anti-Human CD163 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1607) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF109). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system." class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to fibroblasts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

CD163 was detected in immersion fixed paraffin-embedded sections of Human Kidney using Goat Anti-Human CD163 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1607) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to fibroblasts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

CD163 overexpression does not compensate for the absence of FcRn.A Western blot showing gCD163 and gFcRn expression in wild-type (WT) MA-104 cells, overexpression of gCD163 in MA-104 delta FcRn cells, and gFcRn overexpression in MA-104 delta CD163 cells. B Kinetics of SHFV production from cells in (A) after inoculation with SHFV, MOI of 3. Graph shows mean ± SEM from three independent experiments. All plots include error bars; no error bars are shown when the SEM was smaller than the size of the symbols. C Cells from A were infected with SHFV (MOI = 30) and assayed for intracellular SHFV RNA 4 h later using single molecule RNA fluorescence in situ hybridization (smRNA FISH). Confocal microscopy (×600 magnification) was used to capture 17–21 fields of view taken at random. From each field of view, the percent viral RNA positive cells, average number of puncta per infected cell, and average puncta FISH intensity per infected cell were determined and graphed as independent points. Error bars represent the mean ± SEM. Note that data for the WT condition is the same as that shown in Fig. 4C. Data shows results from one experiment. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/39112502), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1607U-100UG
Alexa Fluor 405	AF1607V-100UG
Alexa Fluor 488	AF1607G-100UG
Alexa Fluor 532	AF1607X-100UG
Alexa Fluor 594	AF1607T-100UG
Alexa Fluor 647	AF1607R-100UG
Alexa Fluor 700	AF1607N-100UG
Alexa Fluor 750	AF1607S-100UG
Alexa Fluor Plus 405	AF1607AFP405-100UG
Alexa Fluor Plus 488	AF1607AFP488-100UG
Alexa Fluor Plus 555	AF1607AFP555-100UG
Alexa Fluor Plus 594	AF1607AFP594-100UG
Alexa Fluor Plus 647	AF1607AFP647-100UG
Alexa Fluor Plus 680	AF1607AFP680-100UG
Biotin	BAF1607

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1607-SP

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NBP2-39099

CD163 Antibody (ED2) - BSA Free

(7 Publications)

100 μg

NBP2-39099

Immunohistochemistry-Frozen: CD163 Antibody (ED2) [NBP2-39099] - Staining of acetone fixed, cryostat sectioned rat spleen.

Immunohistochemistry-Frozen: CD163 Antibody (ED2) [NBP2-39099] - Immunoperoxidase staining of rat lymph node cryosection (NBP2-39099) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Low power.

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-39099AF350
Alexa Fluor 405	NBP2-39099AF405
Alexa Fluor 488	NBP2-39099AF488
Alexa Fluor 532	NBP2-39099AF532
Alexa Fluor 594	NBP2-39099AF594
Alexa Fluor 647	NBP2-39099AF647
Alexa Fluor 700	NBP2-39099AF700
Alexa Fluor 750	NBP2-39099AF750
Alexa Fluor Plus 405	NBP2-39099AFP405
Alexa Fluor Plus 488	NBP2-39099AFP488
Alexa Fluor Plus 555	NBP2-39099AFP555
Alexa Fluor Plus 594	NBP2-39099AFP594
Alexa Fluor Plus 647	NBP2-39099AFP647
Alexa Fluor Plus 680	NBP2-39099AFP680
Allophycocyanin	NBP2-39099APC-100ul
Allophycocyanin/Cy7	NBP2-39099APCCY7
Biotin	NBP2-39099B-100ul
CoraFluor 1	NBP2-39099CL1
DyLight 350	NBP2-39099UV-100ul
DyLight 405	NBP2-39099V-100ul
DyLight 488	NBP2-39099G-100ul
DyLight 550	NBP2-39099R-100ul
DyLight 594	NBP2-39099DL594
DyLight 650	NBP2-39099C-100ul
DyLight 680	NBP2-39099FR-100ul
DyLight 755	NBP2-39099IR-100ul
FITC	NBP2-39099F-100ul
HRP	NBP2-39099H-100ul
Janelia Fluor 525	NBP2-39099JF525
Janelia Fluor 549	NBP2-39099JF549
Janelia Fluor 646	NBP2-39099JF646
Janelia Fluor 669	NBP2-39099JF669
PE	NBP2-39099PE-100ul
PE/Atto594	NBP2-39099PEATT594
PE/Cy5.5	NBP2-39099PECY55
PE/Cy7	NBP2-39099PECY7
PerCP	NBP2-39099PCP-100ul
mFluor Violet 450 SE	NBP2-39099MFV450
mFluor Violet 500 SE	NBP2-39099MFV500
mFluor Violet 610 SE	NBP2-39099MFV610

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NBP1-30148

CD163 Antibody (K20-T)

(2 Reviews)

(9 Publications)

0.1 ml

NBP1-30148

Immunocytochemistry/Immunofluorescence: CD163 Antibody (K20-T) [NBP1-30148] - Human primary macrophages in 3D cell culture. Primary antibodies to CD163 (NBP1-30148; RED) and CD206 (clone 15-2; GREEN) were both diluted at 1:100 in blocking buffer and incubated overnight at 4C. This image represents a 100-micron Z-Stack displayed as maximum intensity projection. Image from verified customer review.

Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148] - Formalin fixed, paraffin embedded human tissues (4 um sections) stained with CD163 Antibody (K20-T).

Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148] - Human bone marrow .

Immunohistochemistry-Paraffin: CD163 Antibody (K20-T) [NBP1-30148] - CD163-positive macrophages in the abscess of the liver. FFPE human tissues (4 um sections) stained with anti-CD163

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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MAB1607

CD163 Antibody (215927) [Unconjugated]

(13 Publications)

100 μg

MAB1607

FAB3832P) and either (A) Mouse Anti-Human CD163 Monoclonal Antibody (Catalog # MAB1607) or (B) isotype control antibody (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

PBMC were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # <a class=

FAB3832P) and either (A) Mouse Anti-Human CD163 Monoclonal Antibody (Catalog # MAB1607) or (B) isotype control antibody (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." alt="PBMC were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human CD163 Monoclonal Antibody (Catalog # MAB1607) or (B) isotype control antibody (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1607U-100UG
Alexa Fluor 405	FAB1607V-100UG
Alexa Fluor 488	FAB1607G-025
Alexa Fluor 488	FAB1607G-100
Alexa Fluor 594	FAB1607T-100UG
Alexa Fluor 647	FAB1607R-100UG
Alexa Fluor 700	FAB1607N-100UG
Alexa Fluor 750	FAB1607S-100UG
Alexa Fluor Plus 405	FAB1607AFP405-100UG
Alexa Fluor Plus 488	FAB1607AFP488-100UG
Alexa Fluor Plus 555	FAB1607AFP555-100UG
Alexa Fluor Plus 594	FAB1607AFP594-100UG
Alexa Fluor Plus 647	FAB1607AFP647-100UG
Alexa Fluor Plus 680	FAB1607AFP680-100UG
Allophycocyanin	FAB1607A-100
Allophycocyanin	FAB1607A-025
PerCP (Peridinin-chlorophyll Protein Complex)	FAB1607C-100
PerCP (Peridinin-chlorophyll Protein Complex)	FAB1607C-025
Phycoerythrin	FAB1607P-100
Phycoerythrin	FAB1607P-025

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB1607N-100UG
Carrier Free	FAB1607A-025
Carrier Free	FAB1607S-100UG
Carrier Free	FAB1607T-100UG
Carrier Free	FAB1607U-100UG
Carrier Free	FAB1607V-100UG
Carrier Free	FAB1607P-025
Carrier Free	FAB1607P-100
Carrier Free	FAB1607A-100
Carrier Free	MAB1607-SP
Carrier Free	FAB1607G-025
Carrier Free	FAB1607G-100
Carrier Free	FAB1607C-025
Carrier Free	FAB1607C-100
Carrier Free	MAB1607-500
Carrier Free	FAB1607R-100UG

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BM4041

CD163 Antibody (5C6-FAT) - Azide and BSA Free

(7 Publications)

0.1 mg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	BM4041AF350
Alexa Fluor 405	BM4041AF405
Alexa Fluor 488	BM4041AF488
Alexa Fluor 532	BM4041AF532
Alexa Fluor 594	BM4041AF594
Alexa Fluor 647	BM4041AF647
Alexa Fluor 700	BM4041AF700
Alexa Fluor 750	BM4041AF750
Alexa Fluor Plus 405	BM4041AFP405
Alexa Fluor Plus 488	BM4041AFP488
Alexa Fluor Plus 555	BM4041AFP555
Alexa Fluor Plus 594	BM4041AFP594
Alexa Fluor Plus 647	BM4041AFP647
Alexa Fluor Plus 680	BM4041AFP680
Allophycocyanin	BM4041APC
CoraFluor 1	BM4041CL1
DyLight 350	BM4041UV
DyLight 405	BM4041V
DyLight 488	BM4041G
DyLight 550	BM4041R
DyLight 594	BM4041DL594
DyLight 650	BM4041C
DyLight 680	BM4041FR
DyLight 755	BM4041IR
HRP	BM4041H
Janelia Fluor 549	BM4041JF549
Janelia Fluor 646	BM4041JF646
Janelia Fluor 669	BM4041JF669
PE	BM4041PE
PE/Atto594	BM4041PEATT594
PE/Cy5.5	BM4041PECY55
PE/Cy7	BM4041PECY7
PerCP	BM4041PCP
mFluor Violet 450 SE	BM4041MFV450
mFluor Violet 500 SE	BM4041MFV500
mFluor Violet 610 SE	BM4041MFV610

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NBP3-07981

CD163 Antibody (CL10652) - BSA Free

100 ul

NBP3-07981

Immunohistochemistry-Paraffin: CD163 Antibody (CL10652) [NBP3-07981] - Staining of rat cerebral cortex shows strong cytoplasmic positivity in perivascular macrophages.

Immunohistochemistry-Paraffin: CD163 Antibody (CL10652) [NBP3-07981] - Staining of human cerebellum shows moderate cytoplasmic positivity in microglia and strong staining in perivascular macrophages.

Immunohistochemistry-Paraffin: CD163 Antibody (CL10652) [NBP3-07981] - Staining of human cerebral cortex (Alzheimer's disease) shows strong cytoplasmic positivity in perivascular macrophages and in microglia.

Immunohistochemistry-Paraffin: CD163 Antibody (CL10652) [NBP3-07981] - Staining of human liver shows strong cytoplasmic positivity in Kupffer cells.

Immunohistochemistry-Paraffin: CD163 Antibody (CL10652) [NBP3-07981] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: CD163 Antibody (CL10652) [NBP3-07981] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.

Reactivity:

Hu, Rt

Species Glossary

Reactivity: Hu, Rt

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-43871AF350
Alexa Fluor 405	NBP3-43871AF405
Alexa Fluor 488	NBP3-43871AF488
Alexa Fluor 532	NBP3-43871AF532
Alexa Fluor 594	NBP3-43871AF594
Alexa Fluor 647	NBP3-43871AF647
Alexa Fluor 700	NBP3-43871AF700
Alexa Fluor 750	NBP3-43871AF750
Alexa Fluor Plus 405	NBP3-43871AFP405
Alexa Fluor Plus 488	NBP3-43871AFP488
Alexa Fluor Plus 555	NBP3-43871AFP555
Alexa Fluor Plus 594	NBP3-43871AFP594
Alexa Fluor Plus 647	NBP3-43871AFP647
Alexa Fluor Plus 680	NBP3-43871AFP680
Allophycocyanin	NBP3-43871APC
Biotin	NBP3-43871B
CoraFluor 1	NBP3-43871CL1
DyLight 350	NBP3-43871UV
DyLight 405	NBP3-43871V
DyLight 488	NBP3-43871G
DyLight 550	NBP3-43871R
DyLight 594	NBP3-43871DL594
DyLight 650	NBP3-43871C
DyLight 680	NBP3-43871FR
DyLight 755	NBP3-43871IR
FITC	NBP3-43871F
HRP	NBP3-43871H
Janelia Fluor 525	NBP3-43871JF525
Janelia Fluor 549	NBP3-43871JF549
Janelia Fluor 646	NBP3-43871JF646
Janelia Fluor 669	NBP3-43871JF669
PE	NBP3-43871PE
PerCP	NBP3-43871PCP
mFluor Violet 450 SE	NBP3-43871MFV450
mFluor Violet 500 SE	NBP3-43871MFV500
mFluor Violet 610 SE	NBP3-43871MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-43871
BSA Free	NBP3-07981-25ul

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NBP2-36495

CD163 Antibody (6D3.2F3) - BSA Free

(1 Publication)

0.1 mg

NBP2-36495

Reactivity:

Hu, Pm

Species Glossary

Reactivity: Hu, Pm

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-36495AF350
Alexa Fluor 405	NBP2-36495AF405
Alexa Fluor 488	NBP2-36495AF488
Alexa Fluor 532	NBP2-36495AF532
Alexa Fluor 594	NBP2-36495AF594
Alexa Fluor 647	NBP2-36495AF647
Alexa Fluor 700	NBP2-36495AF700
Alexa Fluor 750	NBP2-36495AF750
Alexa Fluor Plus 405	NBP2-36495AFP405
Alexa Fluor Plus 488	NBP2-36495AFP488
Alexa Fluor Plus 555	NBP2-36495AFP555
Alexa Fluor Plus 594	NBP2-36495AFP594
Alexa Fluor Plus 647	NBP2-36495AFP647
Alexa Fluor Plus 680	NBP2-36495AFP680
Allophycocyanin	NBP2-36495APC
Biotin	NBP2-36495B
CoraFluor 1	NBP2-36495CL1
DyLight 350	NBP2-36495UV
DyLight 405	NBP2-36495V
DyLight 488	NBP2-36495G
DyLight 550	NBP2-36495R
DyLight 594	NBP2-36495DL594
DyLight 650	NBP2-36495C
DyLight 680	NBP2-36495FR
DyLight 755	NBP2-36495IR
FITC	NBP2-36495F
HRP	NBP2-36495H
Janelia Fluor 525	NBP2-36495JF525
Janelia Fluor 549	NBP2-36495JF549
Janelia Fluor 646	NBP2-36495JF646
Janelia Fluor 669	NBP2-36495JF669
PE	NBP2-36495PE
PerCP	NBP2-36495PCP
mFluor Violet 450 SE	NBP2-36495MFV450
mFluor Violet 500 SE	NBP2-36495MFV500
mFluor Violet 610 SE	NBP2-36495MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-36495SS
Azide and BSA Free	NBP2-80632

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NBP1-60158

CD163 Antibody (RM3/1)

(5 Publications)

0.05 mg

NBP1-60158

Reactivity:

Hu, Pm

Species Glossary

Reactivity: Hu, Pm

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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MAB11745

CD163 Antibody (1113338) [Unconjugated]

100 μg

MAB11745

DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder." class="big_lightbox">

CD163 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rat Anti-Mouse CD163, Monoclonal Antibody (Catalog # MAB11745) at 3 μg/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder." alt="CD163 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rat Anti-Mouse CD163, Monoclonal Antibody (Catalog # MAB11745) at 3 μg/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder." class="big_thumb" />

HAF005). A specific band was detected for CD163 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Western Blot shows lysates of mouse spleen tissue. PVDF membrane was probed with 2 µg/ml of Rat Anti-Mouse CD163 Monoclonal Antibody (Catalog # MAB11745) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # <a class=

HAF005). A specific band was detected for CD163 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

CD163 was detected in perfusion fixed paraffin-embedded sections of mouse colon using Rat Anti-Mouse CD163 Monoclonal Antibody (Catalog # MAB11745) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # <a class=

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface of Kupffer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

CD163 was detected in perfusion fixed paraffin-embedded sections of mouse liver using Rat Anti-Mouse CD163 Monoclonal Antibody (Catalog # MAB11745) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # <a class=

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface of Kupffer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB11745-SP

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NBP2-48846

CD163 Antibody - BSA Free

0.1 ml

NBP2-48846

Immunohistochemistry-Paraffin: CD163 Antibody [NBP2-48846] - Staining of human liver shows strong membranous positivity in Kupffer cells.

Immunohistochemistry-Paraffin: CD163 Antibody [NBP2-48846] - Staining of human duodenum shows strong membranous positivity in lymphoid cells.

<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD163 Antibody [NBP2-48846] - Staining of human placenta shows strong membranous positivity in lymphoid cells.

<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CD163 Antibody [NBP2-48846] - Staining of human spleen shows strong membranous positivity in cells in red pulp.

Immunohistochemistry-Paraffin: CD163 Antibody [NBP2-48846] - Staining of human skeletal muscle shows low positivity as expected.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-48846-25ul

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

CD163 Antibodies

CD163 Antibody (EDHu-1) - BSA Free

CD163 Antibody (10D6)

CD163 Antibody [Unconjugated]

CD163 Antibody (ED2) - BSA Free

CD163 Antibody (K20-T)

CD163 Antibody (215927) [Unconjugated]

CD163 Antibody (5C6-FAT) - Azide and BSA Free

CD163 Antibody (CL10652) - BSA Free

CD163 Antibody (6D3.2F3) - BSA Free

CD163 Antibody (RM3/1)

CD163 Antibody (1113338) [Unconjugated]

CD163 Antibody - BSA Free