CD11c Antibody (N418) - Chimeric - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: CD11c Antibody (N418) [NBP2-81066] - Immunofluorescence staining of fixed U937 cells with anti-CD11c antibody N418 [NBP2-81066]. Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of N418 [NBP2-81066] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81066, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Mu
• Applications: ELISA, ICC/IF
• Clone: N418
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

CD11c Antibody (N418) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit CD11c Antibody (N418) - Chimeric - Azide and BSA Free (NBP2-81066) is a recombinant monoclonal antibody validated for use in ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Hamster spleen cells that had been primed to mouse spleen dendritic cells
• Specificity: The antibody binds to mouse and human CD11c.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ITGAX
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG1 format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to human CD11, which is part of the human integrin receptor for fibrinogen, recognizing a GPR motif. The receptor is involved in cell-cell contacts, chemotaxis, and inflammatory responses.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD11c Antibody (N418) - Chimeric - Azide and BSA Free

• 95 alpha chain
• 95
• 95, alpha subunit
• CD11 antigen-like family member C
• CD11c antigen
• CD11c
• CD11Cleu M5, alpha subunit
• Integrin alpha X
• integrin alpha-X
• integrin, alpha X (antigen CD11C (p150), alpha polypeptide)
• integrin, alpha X (complement component 3 receptor 4 subunit)
• ITGAX
• Leu M5
• Leukocyte adhesion glycoprotein p150
• Leukocyte adhesion receptor p150
• leukocyte surface antigen p150
• myeloid membrane antigen, alpha subunit
• p150 95 integrin alpha chain
• p150,95 alpha
• SLEB6

Background

CD11c encodes the integrin alpha X chain protein. CD11c is a type I transmembrane protein found at high levels on most human dendritic cells, but also on monocytes, macrophages, neutrophils, and some B cells that induces cellular activation and helps trigger neutrophil respiratory burst; expressed in hairy cell leukemias, acute nonlymphocytic leukemias, and some B-cell chronic lymphocytic leukemias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGAX