Human CD117/c-kit ELISA Kit (Colorimetric)

ELISA: CD117/c-kit ELISA Kit [NBP2-50066] - Typical Standard Curve: These standard curves are for demonstration only. A standard curve must be run with each assay.

Product Discontinued

Human CD117/c-kit ELISA Kit (Colorimetric) Summary

• Description: This kit is a sandwich ELISA assay for the quantitative measurement of human c-Kit in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of c-Kit captured from the samples.
• Standard Curve Range: 0.781 - 50 ng/mL
• Sensitivity: < 0.469 ng/mL
• Assay Type: Sandwich ELISA
• Sample Volume: 0.1 mL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: KIT

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Detection Method: Absorbance (450 nm). Quantitative measurement of c-Kit in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Alternate Names for Human CD117/c-kit ELISA Kit (Colorimetric)

• CD117 antigen
• CD117
• ckit
• c-kit
• EC 2.7.10
• EC 2.7.10.1
• KIT
• PBT
• piebald trait
• Proto-oncogene c-Kit
• proto-oncogene tyrosine-protein kinase Kit
• SCF R
• SCFR
• SCFRmast/stem cell growth factor receptor
• soluble KIT variant 1
• Tyrosine-protein kinase Kit
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

Tyrosine-protein kinase acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, c-Kit can activate several signaling pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for CD117/c-kit ELISA Kit (NBP2-50066). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: KIT