Human CD117/c-kit ELISA Kit (Colorimetric)

Images

 
ELISA: CD117/c-kit ELISA Kit [NBP2-50066] - Typical Standard Curve: These standard curves are for demonstration only. A standard curve must be run with each assay.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Human serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
Standard Curve Range
0.781 - 50 ng/mL
Sensitivity
< 0.469 ng/mL

Order Details

Human CD117/c-kit ELISA Kit (Colorimetric) Summary

Description
This kit is a sandwich ELISA assay for the quantitative measurement of human c-Kit in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of c-Kit captured from the samples.
Standard Curve Range
0.781 - 50 ng/mL
Sensitivity
< 0.469 ng/mL
Assay Type
Sandwich ELISA
Sample Volume
0.1 mL
Kit Type
ELISA Kit (Colorimetric)
Gene
KIT

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
Detection Method: Absorbance (450 nm). Quantitative measurement of c-Kit in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Antibody dilution buffer
  2. Biotin- detection antibody (Concentrated)
  3. HRP-Streptavidin Conjugate (SABC)
  4. Lyophilized Standard
  5. Microplate: 96 wells
  6. Plate sealers
  7. SABC dilution buffer
  8. Sample / Standard dilution buffer
  9. Stop Solution
  10. TMB substrate
  11. Wash buffer (25X)

Alternate Names for Human CD117/c-kit ELISA Kit (Colorimetric)

  • CD117 antigen
  • CD117
  • ckit
  • c-kit
  • EC 2.7.10
  • EC 2.7.10.1
  • KIT
  • PBT
  • piebald trait
  • Proto-oncogene c-Kit
  • proto-oncogene tyrosine-protein kinase Kit
  • SCF R
  • SCFR
  • SCFRmast/stem cell growth factor receptor
  • soluble KIT variant 1
  • Tyrosine-protein kinase Kit
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

Tyrosine-protein kinase acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, c-Kit can activate several signaling pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Blogs on CD117/c-kit.

Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene ...  Read full blog post.

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Bioinformatics

Gene Symbol KIT