CD117/c-kit Antibody

Product Discontinued

CD117/c-kit Antibody Summary

• Immunogen: A synthetic peptide from C-terminus of human CD117/c-kit protein.
• Localization: Cell membrane, cytoplasm
• Marker: Hematopoietic Stem Cell Marker
• Specificity: This antibody reacts with a 145 kDa protein. c-kits are transmembrane receptor tyrosine kinases expressed in many tissues and cells and are involved in the development of several lineages of stem cells such as germ cells, neural crest derived melanocytes, and hematopoietic precursor cells.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KIT
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: IHC:For an ABC system, dilute 1:50-100 and incubate for 30-60 minutes at RT. For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval before commencing with IHC.

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: EDTA, pH 8.0, containing less than 0.1% sodium azide
• Preservative: Sodium Azide
• Purity: Affinity purified

Alternate Names for CD117/c-kit Antibody

• CD117 antigen
• CD117
• ckit
• c-kit
• EC 2.7.10
• EC 2.7.10.1
• KIT
• PBT
• piebald trait
• Proto-oncogene c-Kit
• proto-oncogene tyrosine-protein kinase Kit
• SCF R
• SCFR
• SCFRmast/stem cell growth factor receptor
• soluble KIT variant 1
• Tyrosine-protein kinase Kit
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

c-Kit is a transmembrane tyrosine kinase encoded by the cKit proto oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Mutations in c-Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma and lung cancer. Phosphorylation at tyrosine 721 of c-Kit allows binding and activation of PI3 kinase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD117/c-kit Antibody (NB120-956)(2)

Publications using NB120-956

• Miller DD, Emley A, Yang S et al. Mixed versus pure variants of desmoplastic melanoma: a genetic and immunohistochemical appraisal. Mod Pathol;25(4):505-15. 2012-04-01 [PMID: 22157936] (IF/IHC, Human)
• Park E, Yang S, Emley A et al. Lack of correlation between immunohistochemical expression of CKIT and KIT mutations in atypical acral nevi. Am J Dermatopathol 2012-02-01 [PMID: 22094233] (Human)

FAQs for CD117/c-kit Antibody (NB120-956). (Showing 1 - 1 of 1 FAQs).

1. What is the concentratin of lot T876?
   • The immunoglobulin concentration of lot T876 is 0.135 mg/ml.

Bioinformatics

• Gene Symbol: KIT
• Entrez:
  • 3815(Human)
• Uniprot:
  • P10721()