CD117/c-kit Antibody (DMC446) - Chimeric

• Reactivity: Hu
• Applications: Flow
• Clone: DMC446
• Clonality: Monoclonal
• Host: Human
• Conjugate: Unconjugated

CD117/c-kit Antibody (DMC446) - Chimeric Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: CD117/c-kit recombinant protein expressed in mammalian cells (Accession# P10721). The exact amino acid sequence is proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Human
• Gene: KIT
• Purity: Antigen Affinity-purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at -20C prior to opening. Aliquot reconstituted liquid and and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing.
• Buffer: PBS, pH 7.4
• Preservative: Trehalose
• Purity: Antigen Affinity-purified from cell culture supernatant

Alternate Names for CD117/c-kit Antibody (DMC446) - Chimeric

• CD117 antigen
• CD117
• ckit
• c-kit
• EC 2.7.10
• EC 2.7.10.1
• KIT
• PBT
• piebald trait
• Proto-oncogene c-Kit
• proto-oncogene tyrosine-protein kinase Kit
• SCF R
• SCFR
• SCFRmast/stem cell growth factor receptor
• soluble KIT variant 1
• Tyrosine-protein kinase Kit
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KIT