CD117/c-kit Antibody (180627) [Allophycocyanin]

Mouse splenocytes gated on hematopoietic lineage negative cells were stained with Rat Anti-Mouse CD117/c-kit APC-conjugated Monoclonal Antibody (Catalog # FAB1356A) and Rat Anti-Mouse Sca-1/Ly6 PE-conjugated Monoclonal Antibody (Catalog # FAB1226P). Quadrant markers were set based on control antibody staining (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 180627
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Allophycocyanin

CD117/c-kit Antibody (180627) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD117/c-kit
  Gln25-Thr519 (Ala207Glu)
  Accession # P05532
• Specificity: Detects mouse CD117/c-kit in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: KIT
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD117/c-kit Antibody (180627) [Allophycocyanin]

• CD117 antigen
• CD117
• ckit
• c-kit
• EC 2.7.10
• EC 2.7.10.1
• KIT
• PBT
• piebald trait
• Proto-oncogene c-Kit
• proto-oncogene tyrosine-protein kinase Kit
• SCF R
• SCFR
• SCFRmast/stem cell growth factor receptor
• soluble KIT variant 1
• Tyrosine-protein kinase Kit
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

SCF R is also known as the c-kit proto-oncogene protein and as CD117. Together with its ligand, stem cell factor (also called c-kit ligand or mast cell growth factor), SCF R plays an essential role in gametogenesis, melanogenesis, and hematopoiesis. SCF R is expressed in hematopoietic progenitor cells, normal B  and T lymphocyte progenitors, mast cells, germ cells, melanocytes, neurons, glial cells, placenta, kidney, lung, and gut. In addition, SCF R expression has also been reported in several human tumor cell lines (1,2).

1. Broudy, V. (1997) Blood 90:1345.
2. Vliagoftis, H. et al. (1997) J. Allergy Clin. Immunol. 100:435.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD117/c-kit Antibody (FAB1356A)(1)

Publication using FAB1356A

• ST Babu, X Niu, M Raetz, RC Savani, LV Hooper, J Mirpuri Maternal high-fat diet results in microbiota-dependent expansion of ILC3s in mice offspring JCI Insight, 2018-10-04;3(19):. 2018-10-04 [PMID: 30282818] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: KIT
• Uniprot:
  • P05532()