CCR9 Antibody - BSA Free

Immunohistochemistry-Paraffin: CCR9 Antibody [NB100-708] - Analysis using the Biotin conjugate of NB100-708. Staining of mouse spleen.

Product Discontinued

CCR9 Antibody - BSA Free Summary

• Description: This product is likely to be neutralizing because the antibody to similiar domains of human CCR9 block TECK induced activation of ES-2 cells.
• Immunogen: Synthetic peptide: IPGMFDDFSYDSTASTDDYMNLNFSSFF, corresponding to amino acids 10-37 of Mouse CCR9.
• Localization: Cell Membrane
• Specificity: Peptide sequence is < 50 % identical to other mouse chemokine receptors in this region.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCR9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:125
  • Immunohistochemistry-Paraffin 1:125
• Application Notes: ELISA: Use at a dilution of 1/100,000. Flow Cytometry Use at a dilution of 1/200. ICC: Use at a dilution of 1/500. IHC-P: Use at a dilution of 1/250. WB: Use at a dilution of 1/1000. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Likely to cross-react with human CCR9 with approximately 50% less titer.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM KHPO4 and 0.14M NaCl
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

This product is likely to be neutralizing because the antibody to similiar domains of human CCR9 block TECK induced activation of ES-2 cells.

Alternate Names for CCR9 Antibody - BSA Free

• C-C chemokine receptor type 9
• C-C CKR-9
• CC-CKR-9
• CCR9
• CCR-9
• CD199
• CDw199 antigen
• CDw199
• chemokine (C-C motif) receptor 9
• G protein-coupled receptor 28
• GPR28
• GPR-9-6G-protein coupled receptor 28

Background

The protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. This gene is expressed in a range of tissues and hemopoietic cells. The expression of this receptor in lymphatic endothelial cells and overexpression in vascular tumors suggested its function in chemokine-driven recirculation of leukocytes and possible chemokine effects on the development and growth of vascular tumors. This receptor appears to bind the majority of beta-chemokine family members; however, its specific function remains unknown. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to chromosome 3p21.3, a region that includes a cluster of chemokine receptor genes. Two alternatively spliced transcript variants have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR9 Antibody (NB100-708)(1)

Publication using NB100-708

• Fu H, Jangani M, Parmar A et al. A Subset of CCL25-Induced Gut-Homing T Cells Affects Intestinal Immunity to Infection and Cancer Front. Immunol. 2019-02-25 [PMID: 30863398] (FLOW, Mouse)

Bioinformatics

• Gene Symbol: CCR9
• Entrez:
  • 12769(Mouse)
• Uniprot:
  • Q9WUT7()