NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="CCR9 was detected in immersion fixed MOLT-4 human acute lymphoblastic leukemia cell line using Mouse Anti-Human CCR9 Monoclonal Antibody (Catalog # MAB179) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />
MAB0061, open histogram) followed by Goat anti-Rat IgG APC-conjugated Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins." alt="Mouse thymocytes were stained with Rat Anti-Mouse CCR9 Monoclonal Antibody (Catalog # MAB2160, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram) followed by Goat anti-Rat IgG APC-conjugated Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26685-50ul | |||
MAB003) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." alt="MOLT-4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human CCR9 Monoclonal Antibody (Catalog # MAB1791, filled histogram) or Mouse IgG2A Flow Cytometry Isotype Control (Catalog # MAB003) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />
F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041)." alt="HEK293 human embryonic kidney cell line transfected with (A) rat CCR9 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Rat CCR9 Monoclonal Antibody (Catalog # MAB8287) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB8287-SP | |||
| Carrier Free | FAB8287V-100UG | |||
| Carrier Free | FAB8287U-100UG | |||
| Carrier Free | FAB8287T-100UG | |||
| Carrier Free | FAB8287S-100UG | |||
| Carrier Free | FAB8287R-100UG | |||
| Carrier Free | FAB8287N-100UG | |||
| Carrier Free | FAB8287G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21617-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12001-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12002-0.025mg | |||
MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ![Immunoprecipitation: CCR9 Antibody (4G2) [NBP3-26685] - Immunoprecipitating CCR9 in HL-60 whole cell lysate.<br /> Lane 1: Rabbit control IgG instead of NBP3-26685 in HL-60 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).<br /> Lane 2: NBP3-26685 (3ug) + HL-60 whole cell lysate (500ug).<br /> Lane 3: HL-60 whole cell lysate (20ug).<br />](http://images.novusbio.com/fullsize/nbp3-26685_rabbit-ccr9-mab-4g2-262202413221517.jpg "Immunoprecipitation: CCR9 Antibody (4G2) [NBP3-26685] - Immunoprecipitating CCR9 in HL-60 whole cell lysate.<br /> Lane 1: Rabbit control IgG instead of NBP3-26685 in HL-60 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).<br /> Lane 2: NBP3-26685 (3ug) + HL-60 whole cell lysate (500ug).<br /> Lane 3: HL-60 whole cell lysate (20ug).<br />")
![Western Blot: CCR9 Antibody (4G2) [NBP3-26685] - Positive Western Blot detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, HL-60 whole cell lysate, Hela whole cell lysate, U87 whole cell lysate, Mouse brain tissue.<br /> All lanes: CCR9 Antibody at 1ug/ml.<br /> Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.<br /> Predicted band size: 43, 41 KDa<br /> Observed band size: 43 KDa<br />](http://images.novusbio.com/fullsize/nbp3-26685_rabbit-ccr9-mab-4g2-282202411472120.jpg)
![Immunocytochemistry/Immunofluorescence: CCR9 Antibody (4G2) [NBP3-26685] - Staining of HepG2 cells with CCR9 Antibody (4G2) at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26685_rabbit-ccr9-mab-4g2-28220241312217.jpg)
NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane and cytoplasm. View our protocol for 
MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ![Western Blot: CCR9 Antibody (SR1763) [NBP3-21617] - Western blot analysis using NBP3-21617 in (1) A549 cell lysate; (2) Daudi cell lysate.](http://images.novusbio.com/fullsize/nbp3-21617_rabbit-ccr9-mab-sr1763-5720231658215.jpg "Western Blot: CCR9 Antibody (SR1763) [NBP3-21617] - Western blot analysis using NBP3-21617 in (1) A549 cell lysate; (2) Daudi cell lysate.")
![Flow Cytometry: Mouse CCR9 mAb (C9Mab-1) [NBP3-14623] - Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD199 (C9Mab-1) purified antibody (concentration in sample 0.5 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/antibody/nbp3-14623_mouse-ccr9-mab-c9mab-1-flow-cytometry-1212202414204..jpg "Flow Cytometry: Mouse CCR9 mAb (C9Mab-1) [NBP3-14623] - Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD199 (C9Mab-1) purified antibody (concentration in sample 0.5 ug/ml, GAM APC).")
![Flow Cytometry: Mouse CCR9 mAb (C9Mab-1) [NBP3-14623] - Separation of human CD20 positive lymphocytes (red-filled) from CD20 negative CD199 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD199 (C9Mab-1) purified antibody (concentration in sample 0.5 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/antibody/nbp3-14623_mouse-ccr9-mab-c9mab-1-flow-cytometry-12122024141531.jpg)
![Flow Cytometry: Mouse CCR9 mAb (C9Mab-1) [NBP3-14623] - Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD199 (C9Mab-1) purified antibody (concentration in sample 0.5 ug/ml, GAM APC) and anti-human CD20 (2H7) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood).](http://images.novusbio.com/fullsize/antibody/nbp3-14623_mouse-ccr9-mab-c9mab-1-flow-cytometry-12122024141756..jpg)
![Flow Cytometry: CCR9 Antibody (9B1) - Chimeric - Azide and BSA Free [NBP3-12001] - Paraformaldehyde fixed mouse splenocytes were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12001 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12001_rabbit-monoclonal-ccr9-antibody-9b1-chimeric-74202522141232.png "Flow Cytometry: CCR9 Antibody (9B1) - Chimeric - Azide and BSA Free [NBP3-12001] - Paraformaldehyde fixed mouse splenocytes were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12001 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Immunocytochemistry /Immunofluorescence: CCR9 Antibody (9B1) - Chimeric - Azide and BSA Free [NBP3-12001] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with NBP3-12001 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP3-12001, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-12001_rabbit-monoclonal-ccr9-antibody-9b1-chimeric-7420252211304.png)
![Western Blot: CCR9 Antibody (4F7Q6) [NBP3-16861] - Western blot analysis of extracts of various cell lines, using CCR9 Rabbit mAb (NBP3-16861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CCR9-Antibody-4F7Q6-Western-Blot-NBP3-16861-img0001.jpg "Western Blot: CCR9 Antibody (4F7Q6) [NBP3-16861] - Western blot analysis of extracts of various cell lines, using CCR9 Rabbit mAb (NBP3-16861) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: CCR9 Overexpression Lysate (Adult Normal) [NBP2-04314] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CCR9.](http://images.novusbio.com/fullsize/CCR9-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04314-img0001.jpg "Western Blot: CCR9 Overexpression Lysate (Adult Normal) [NBP2-04314] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CCR9.")
