Western Blot: CCR10/GPR2 Antibody [NB100-56319] - Analysis for GPR2/CCR10 using NB100-56319 at 2 ug/ml dilution against 10 ug of MCF-7 (a human breast cancer cell line) cell lysate.
Simple Western: CCR10/GPR2 Antibody [NB100-56319] - Simple Western lane view shows a specific band for CCR10 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Rabbit anti-CCR10 polyclonal antibody was raised against a peptide corresponding to amino acids 350-362 of human CCR10. In this region, mouse and human amino acid sequences are 100% identical.
In A375, a ~50 kDa band is observed. The CCR10 antibody is made against an epitope in the cytoplasmic domain of the receptor (Moed et al, 2004). For flow cytometry (intracellular) Moed et al fixed cells with 2% formaldehye and permeabilized with 0.1% saponin.
Protein G purified
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Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS with 0.2% BSA
0.05% Sodium Azide
Protein G purified
Flow Cytometry (Intracellular): see Moed et al, 2004 Immunohistochemistry-Frozen: see Vestergaard et al, 2003. In A375, a ~50 kDa band is observed. The CCR10 antibody is made against an epitope in the cytoplasmic domain of the receptor (Moed et al, 2004). For flow cytometry (intracellular) Moed et al fixed cells with 2% formaldehye and permeabilized with 0.1% saponin. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Read Publications using NB100-56319 in the following applications:
Recently, a function has been assigned to a previously identified orphan chemokine receptor, GPR-2. This receptor, also called CCR10, binds with monocyte chemoattractant protein (MCP)-1, MCP-3 and MIP-1 beta. CCR10 is expressed by melanocytes, dermal fibroblasts, dermal microvascular endothelial cells, T cells as well as in skin-derived Langerhans cells. CCR10 may play a role in both skin homeostasis as well as a potential role in inflammatory responses.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.