CCR10/GPR2 Antibody

Images

 
Western Blot: CCR10/GPR2 Antibody [NB100-56319] - Analysis for GPR2/CCR10 using NB100-56319 at 2 ug/ml dilution against 10 ug of MCF-7 (a human breast cancer cell line) cell lysate.
Simple Western: CCR10/GPR2 Antibody [NB100-56319] - Simple Western lane view shows a specific band for CCR10 in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, IHC-Fr
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

CCR10/GPR2 Antibody Summary

Immunogen
Rabbit anti-CCR10 polyclonal antibody was raised against a peptide corresponding to amino acids 350-362 of human CCR10. In this region, mouse and human amino acid sequences are 100% identical.
Specificity
In A375, a ~50 kDa band is observed. The CCR10 antibody is made against an epitope in the cytoplasmic domain of the receptor (Moed et al, 2004). For flow cytometry (intracellular) Moed et al fixed cells with 2% formaldehye and permeabilized with 0.1% saponin.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CCR10
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Simple Western 1:50
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry-Frozen 1:10-1:2000
Application Notes
Flow Cytometry (Intracellular): see Moed et al, 2004 Immunohistochemistry-Frozen: see Vestergaard et al, 2003. In A375, a ~50 kDa band is observed. The CCR10 antibody is made against an epitope in the cytoplasmic domain of the receptor (Moed et al, 2004). For flow cytometry (intracellular) Moed et al fixed cells with 2% formaldehye and permeabilized with 0.1% saponin.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
CCR10/GPR2 Lysate (NBL1-08888)
Publications
Read Publications using
NB100-56319 in the following applications:

Reactivity Notes

Predicted to react with Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS with 0.2% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for CCR10/GPR2 Antibody

  • CC chemokine receptor 10
  • C-C chemokine receptor type 10
  • C-C CKR-10
  • CC-CKR-10
  • CCR10
  • CCR-10
  • chemokine (C-C motif) receptor 10
  • G protein-coupled receptor 2
  • GPR2
  • GPR2G-protein coupled receptor 2

Background

Recently, a function has been assigned to a previously identified orphan chemokine receptor, GPR-2. This receptor, also called CCR10, binds with monocyte chemoattractant protein (MCP)-1, MCP-3 and MIP-1 beta. CCR10 is expressed by melanocytes, dermal fibroblasts, dermal microvascular endothelial cells, T cells as well as in skin-derived Langerhans cells. CCR10 may play a role in both skin homeostasis as well as a potential role in inflammatory responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCR10/GPR2 Antibody (NB100-56319)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Flow - IC, IHC-Fr, WB.


Filter By Application
Flow - IC
(2)
IHC-Fr
(2)
WB
(2)
All Applications
Filter By Species
Human
(5)
All Species
Showing Publications 1 - 6 of 6.
Publications using NB100-56319 Applications Species
Rodrigues-Lisoni FC, Mehet DK, Peitl P Jr et al. In vitro and in vivo studies on CCR10 regulation by Annexin A1. FEBS Lett. 2006 Feb 20 [PMID: 16460738] (WB)

Details:
WB (human Hep-2 cells): Fig 3a.
WB
Sell H, Dietze-Schroeder D, Kaiser U, Eckel J. Monocyte chemotactic protein-1 is a potential player in the negative cross-talk between adipose tissue and skeletal muscle. Endocrinology. 2006 May [PMID: 16439461] (WB, Human)

Details:
WB: CCR4 (IMG-332) and CCR10 (IMG-286): human skeletal muscle cells and adipocytes (Fig 3).
WB Human
Moed H, Stoof TJ, Boorsma DM et al. Identification of anti-inflammatory drugs according to their capacity to suppress type-1 and type-2 T cell profiles. Clin Exp Allergy. 2004 Dec [PMID: 15663561] (Flow - IC, Human)

Details:
Flow (Intracellular): human PBMC from nickel-allergic patients (Figs 1B, 2B, 3B).
Flow - IC Human
Moed H, Boorsma DM, Tensen CP et al. Increased CCL27-CCR10 expression in allergic contact dermatitis: implications for local skin memory. J Pathol. 2004 Sep [PMID: 15307136] (IHC-Fr, Human)

Details:
IHC-F (human skin biopsies from patients with allergic contact dermatitis): Tables 2,3; Fig. 5.
IHC-Fr Human
Moed H, Boorsma DM, Stoof TJ et al. Nickel-responding T cells are CD4+ CLA+ CD45RO+ and express chemokine receptors CXCR3, CCR4 and CCR10. Br J Dermatol. 2004 Jul [PMID: 15270870] (Flow - IC, Human)

Details:
Flow (Intracellular): human PMBC from nickel-allergic patients (Fig 6).
Flow - IC Human
Vestergaard C, Deleuran M, Gesser B et al. Expression of the T-helper 2-specific chemokine receptor CCR4 on CCR10-positive lymphocytes in atopic dermatitis skin but not in psoriasis skin. Br J Dermatol. 2003 Sep [PMID: 14510975] (IHC-Fr, Human)

Details:
IHC-F (human skin biopsies from patients with atopic dermatitis and psoriasis): Figs 1 and 2, Table 1.
IHC-Fr Human

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CCR10
Entrez
Uniprot