CCL28 Antibody (134306) [Unconjugated]

Images

 
Recombinant Mouse CCL28 (Catalog # 533-VI) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N
Clone
134306
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price

CCL28 Antibody (134306) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse CCL28
Ile23-Arg130
Accession # Q9JIL2
Specificity
Detects mouse CCL28 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human (rh) CCL28 is observed and no cross-reactivity with rhCCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, recombinant mouse CCL1, 2, 3, 4, 6, 7, 9/10, 11, 12, 19, 20, 21, 22, 24, 25, 27, or recombinant rat CCL20 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
CCL28
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Neutralization 2.5-5.0 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB533 in the following applications:

Publications
Read Publications using
MAB533 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL28 Antibody (134306) [Unconjugated]

  • CCL28
  • chemokine (C-C motif) ligand 28
  • MEC
  • member 28
  • MGC71902
  • Protein CCK1
  • VIC

Background

Mouse CCL28 (CC chemokine ligand 28) is a novel CC chemokine cloned from a Rag-1 mouse kidney cDNA library. Mouse CCL28 cDNA encodes a 130 amino acid (aa) residue precursor protein with a putative 22 aa residue signal peptide that is cleaved to produce the 108 aa residue mature protein. Human and mouse CCL28 are highly conserved, sharing 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Mouse CCL28 is produced by epithelial cells. Based on Northern blot analysis, it is mainly expressed in testes, kidney and brain. The receptor for CCL28 has been identified as the CCR10 which is also the receptor for CCL27/CTACK.

  1. Wang, W. et al. (2000) J. Biol. Chem. 275:22313.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-62664
Species: Hu
Applications: IHC, IHC-P
H00008767-M02
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P
NB100-2803
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
MAB1364
Species: Hu
Applications: Flow, CyTOF-ready
AF2680
Species: Hu, Mu, Rt
Applications: WB, IHC
NB100-56319
Species: Hu
Applications: WB, Simple Western, Flow, IHC, IHC-Fr, Flow-CS, Flow-IC
MAB6899
Species: Hu
Applications: Flow, CyTOF-ready
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
MAB208
Species: Hu
Applications: WB, Simple Western, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
NBP2-50599
Species: Hu
Applications: WB, ELISA, Flow, IHC, IP, CyTOF-ready
MAB179
Species: Hu
Applications: Flow, IHC, CyTOF-ready, ICC
MAB155
Species: Hu
Applications: Flow, IHC, CyTOF-ready, Neut

Publications for CCL28 Antibody (MAB533)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: IHC-Fr, Immunoprecipitation, In Vivo, Neutralization.


Filter By Application
IHC-Fr
(1)
Immunoprecipitation
(1)
In Vivo
(1)
Neutralization
(4)
All Applications
Filter By Species
Mouse
(7)
All Species
Showing Publications 1 - 7 of 7.
Publications using MAB533 Applications Species
P Wang, X Qi, G Xu, J Liu, J Guo, X Li, X Ma, H Sun CCL28 promotes locomotor recovery after spinal cord injury via recruiting regulatory T cells Aging (Albany NY), 2019;11(18):7402-7415. 2019 [PMID: 31557129] (Neutralization, Mouse) Neutralization Mouse
BJ Buelow, M Rohlfing, F Jung, GJ Douglas, MH Grayson POL7085 or anti-CCL28 treatment inhibits development of post-paramyxoviral airway disease Immun Inflamm Dis, 2017;5(2):98-108. 2017 [PMID: 28474501] (In Vivo, Mouse) In Vivo Mouse
Thomas M, Buelow B, Nevins A, Jones S, Peterson F, Gundry R, Grayson M, Volkman B Structure-function analysis of CCL28 in the development of post-viral asthma. J Biol Chem, 2015;290(7):4528-36. 2015 [PMID: 25556652] (Neutralization, Mouse) Neutralization Mouse
Cha HR, Ko HJ, Kim ED Mucosa-associated epithelial chemokine/CCL28 expression in the uterus attracts CCR10+ IgA plasma cells following mucosal vaccination via estrogen control. J. Immunol., 2011;187(6):3044-52. 2011 [PMID: 21832166] (Neutralization, Mouse) Neutralization Mouse
Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells. Nature, 2011;475(7355):226-30. 2011 [PMID: 21753853] (IHC-Fr, Mouse) IHC-Fr Mouse
Mosconi E, Rekima A, Seitz-Polski B Breast milk immune complexes are potent inducers of oral tolerance in neonates and prevent asthma development. Mucosal Immunol, 2010;3(5):461-74. 2010 [PMID: 20485331] (Neutralization, Mouse) Neutralization Mouse
Kutzler MA, Kraynyak KA, Nagle SJ, Parkinson RM, Zharikova D, Chattergoon M, Maguire H, Muthumani K, Ugen K, Weiner DB Plasmids encoding the mucosal chemokines CCL27 and CCL28 are effective adjuvants in eliciting antigen-specific immunity in vivo. Gene Ther., 2010;17(1):72-82. 2010 [PMID: 19847203] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse

Review for CCL28 Antibody (MAB533) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using MAB533:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
WB Mouse 02/25/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23021255
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23021255

Details

Detection NotesSee PMID 23021255

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 23021255

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CCL28 Antibody (MAB533) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional CCL28 Products

Bioinformatics Tool for CCL28 Antibody (MAB533)

Discover related pathways, diseases and genes to CCL28 Antibody (MAB533). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CCL28 Antibody (MAB533)

Discover more about diseases related to CCL28 Antibody (MAB533).
 

Pathways for CCL28 Antibody (MAB533)

View related products by pathway.

PTMs for CCL28 Antibody (MAB533)

Learn more about PTMs related to CCL28 Antibody (MAB533).

Blogs on CCL28

There are no specific blogs for CCL28, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

 
Anonymous
02/25/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol CCL28
Uniprot