CCR8 Antibody (JE31-99)

Western Blot: CCR8 Antibody (JE31-99) [NBP3-32117] - Western blot analysis of CCR8 on mouse thymus tissue lysates with Rabbit anti-CCR8 antibody (NBP3-32117) at 1/1,000 dilution. Lysates/proteins at 30 ug/Lane. Predicted band size: 41 kDa Observed band size: 48 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32117) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: CCR8 Antibody (JE31-99) [NBP3-32117] - Immunocytochemistry analysis of Raji cells labeling CCR8 with Rabbit anti-CCR8 antibody (NBP3-32117) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CCR8 antibody (NBP3-32117) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: JE31-99
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

CCR8 Antibody (JE31-99) Summary

• Description: Novus Biologicals Rabbit CCR8 Antibody (JE31-99) (NBP3-32117) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within Human CCR8 aa 281-355 / 355. (Uniprot: P51685)
• Localization: Cell membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CCR8
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Western Blot 1:1000
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CCR8 Antibody (JE31-99)

• CC chemokine receptor 8
• C-C CKR-8
• CC-chemokine receptor chemr1
• CC-CKR-8
• CCR8
• CCR-8
• CD198
• CDw198 antigen
• CDw198
• chemokine (C-C motif) receptor 8
• chemokine (C-C) receptor 8
• chemokine (C-C) receptor-like 2
• Chemokine receptor-like 1
• ChemR1
• CKRL1
• CKR-L1
• CKRL1MGC129966
• CMKBR8MGC129973
• CMKBRL2C-C chemokine receptor type 8
• CY6
• GPRCL1
• GPRCY6
• GPR-CY6
• TER-1
• TER1CC chemokine receptor CHEMR1

Background

CKR8/CCR8, or C-C chemokine receptor type 8, is a G protein-coupled, seven-transmembrane domain receptor protein. Chemokines are key molecules in directing leukocyte migration toward sites of inflammation. The human C-C chemokine I-309 is a monocyte chemoattractant and inhibits apoptosis in thymic cell lines. It has been found that CKR8/CCR8 is a receptor for chemokines SCYA1/I-309, SCYA4/MIP-1-beta, and SCYA17/TARC (1-3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCR8