CCR7 Antibody

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Immunohistochemistry-Paraffin: CCR7 Antibody [NB100-712] - M1 and M2 macrophages showed similar expression patterns in cortical bone. IHC staining was performed in sagittal serial sections of medial condyles from ...read more
Immunohistochemistry-Paraffin: CCR7 Antibody [NB100-712] - Mouse spleen
Immunohistochemistry-Paraffin: CCR7 Antibody [NB100-712] - Mouse spleen
Immunohistochemistry-Paraffin: CCR7 Antibody [NB100-712] - Human spleen

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vivo
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CCR7 Antibody Summary

Immunogen
Synthetic peptide: DPGKPRKNVLVVALLVIFQVC, corresponding to amino acids 2-22 of Mouse CCR7.
Localization
Integral membrane protein.
Specificity
Peptide sequence is < 50 % identical to other mouse chemokine receptors in this region.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCR7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100000
  • Flow Cytometry 1:10
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:250
  • Immunohistochemistry-Frozen 1:250
  • Immunohistochemistry-Paraffin 1:250
  • In vivo assay
  • Western Blot 1:1000
Application Notes
Use in ICC/IF reported in scintific literature (PMID: 29311580). Use in IHC-Frozen reported in scientific literature (PMID: 25803728). Use in In Vivo assays reported in scientific literature (PMID: 30541701).
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Mouse Spleen Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publications using
NB100-712 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 25831137), (25803728).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
10 mM KHPO4, 0.14 M NaCl, 1.0 mg/mL BSA
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CCR7 Antibody

  • BLR2
  • BLR2C-C chemokine receptor type 7
  • C-C CKR-7
  • CC-CKR-7
  • CCR7
  • CCR-7
  • CD197 antigen
  • CD197
  • CDw197
  • CDw197CC chemokine receptor 7
  • chemokine (C-C motif) receptor 7
  • CMKBR7
  • CMKBR7chemokine (C-C) receptor 7
  • EBI1
  • EBI1EBV-induced G protein-coupled receptor 1
  • EBV-induced G-protein coupled receptor 1
  • Epstein-Barr virus induced gene 1
  • Epstein-Barr virus induced G-protein coupled receptor
  • Epstein-Barr virus-induced G-protein coupled receptor 1
  • EVI1
  • lymphocyte-specific G protein-coupled peptide receptor
  • MIP-3 beta receptor

Background

Chemokine receptor 7 (CCR7) is a G-protein coupled receptor (GPCR) that binds chemokine ligand 19 (CCL19) and chemokine ligand 21 (CCL21) and, together, this receptor-ligand interaction functions in inflammatory and adaptive immune response (1,2). The CCR7 protein contains 7-transmembrane spanning alpha helices and is 378 amino acids (aa) in length, with a theoretical molecular weight of 42.8 kDa (3,4). CCR7 is expressed on several cells within the immune system, including naive T cells, central memory T cells, regulatory T cells, naive B cells, a subset of double negative and single positive thymocytes, and mature dendritic cells (DCs) (1, 3).

The primary role of the CCR7/CCL19/CCL21 chemokine signaling axis is homing T cells and DCs to lymph nodes and lymphoid tissues to initiate an immune response (1,2,5,6). In the context of cancer, the CCR7 signaling axis appears to have two opposing roles (2). Downregulation of CCR7 on CD8+ T cells contributes to effector cell migration and anti-cancer activities via cytotoxic tumor-infiltrating lymphocytes (2). However, upregulation of CCR7 by cancer cells can result in cancer cell migration and metastasis (2). Overexpression of CCR7 has been implicated in a variety of cancers including breast, cervical, gastric, head and neck cell carcinoma, and prostate (1,2,7). Studies in breast cancer have found that hypoxia increases CCR7 expression, and this activation can affect cancer cell invasion, extravasation, proliferation, angiogenesis, and metastasis through induction of multiple signaling transduction pathways such as PI3K/AKT, MAPK, and JAK/STAT (5,7).

Given its important role in inflammation and immune response, several strategies have been employed to target the CCR7 signaling axis for cancer immunotherapy (2). Some cancer immunotherapies under investigation include intra-tumoral administration of CCL19 and CCL21, introduction of patient-derived cells transfected to express CCR7 or its ligands, and vaccines (2). Further interrogation of CCR7/CCL19/CCL21 signaling axis is required to develop better therapeutic strategies for cancer treatment.

References:

1. Comerford, I., Harata-Lee, Y., Bunting, M. D., Gregor, C., Kara, E. E., & McColl, S. R. (2013). A myriad of functions and complex regulation of the CCR7/CCL19/CCL21 chemokine axis in the adaptive immune system. Cytokine & growth factor reviews, 24(3), 269-283. https://doi.org/10.1016/j.cytogfr.2013.03.001

2. Salem, A., Alotaibi, M., Mroueh, R., Basheer, H. A., & Afarinkia, K. (2021). CCR7 as a therapeutic target in Cancer. Biochimica et biophysica acta. Reviews on cancer, 1875(1), 188499. https://doi.org/10.1016/j.bbcan.2020.188499

3. Yan, Y., Chen, R., Wang, X., Hu, K., Huang, L., Lu, M., & Hu, Q. (2019). CCL19 and CCR7 Expression, Signaling Pathways, and Adjuvant Functions in Viral Infection and Prevention. Frontiers in cell and developmental biology, 7, 212. https://doi.org/10.3389/fcell.2019.00212

4. Uniprot (P32248)

5. Korbecki, J., Grochans, S., Gutowska, I., Barczak, K., & Baranowska-Bosiacka, I. (2020). CC Chemokines in a Tumor: A Review of Pro-Cancer and Anti-Cancer Properties of Receptors CCR5, CCR6, CCR7, CCR8, CCR9, and CCR10 Ligands. International journal of molecular sciences, 21(20), 7619. https://doi.org/10.3390/ijms21207619

6. Sanchez-Sanchez, N., Riol-Blanco, L., & Rodriguez-Fernandez, J. L. (2006). The multiple personalities of the chemokine receptor CCR7 in dendritic cells. Journal of immunology (Baltimore, Md. : 1950), 176(9), 5153-5159. https://doi.org/10.4049/jimmunol.176.9.5153

7. Rizeq, B., & Malki, M. I. (2020). The Role of CCL21/CCR7 Chemokine Axis in Breast Cancer Progression. Cancers, 12(4), 1036. https://doi.org/10.3390/cancers12041036

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-712
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Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vivo

Publications for CCR7 Antibody (NB100-712)(42)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: FLOW, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In Vivo.


Filter By Application
FLOW
(1)
Flow
(1)
ICC/IF
(10)
IHC
(13)
IHC-Fr
(1)
IHC-P
(4)
In Vivo
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
Rat
(8)
All Species
Showing Publications 1 - 10 of 42. Show All 42 Publications.
Publications using NB100-712 Applications Species
Lian Q, Zheng S, Shi Z et al. Using a degradable three-layer sandwich-type coating to prevent titanium implant infection with the combined efficient bactericidal ability and fast immune remodeling property Acta biomaterialia Oct 25 2022 [PMID: 36306986] (IHC-P, Rat) IHC-P Rat
Hu XH, Li ZH, Muyayalo KP Et al. A newly intervention strategy in preeclampsia: Targeting PD-1/Tim-3 signaling pathways to modulate the polarization of decidual macrophages FASEB journal : official publication of the Federation of American Societies for Experimental Biology Jan 1 2022 [PMID: 34847253]
Guo X, Li M, Qi W Et Al. Serial cellular events in bone formation initiated by calcium phosphate ceramics Acta biomaterialia Jul 22 2021 [PMID: 34303865] (IHC) IHC
Ma Y, Wei C, Qi X et al. Schistosoma japonicum-derived peptide SJMHE1 promotes peripheral nerve repair through a macrophage-dependent mechanism American journal of translational research Mar 15 2021 [PMID: 33841657] (IHC, Rat) IHC Rat
Kim MK, Kim Y, Park S et al. Effects of Steady Low-Intensity Exercise on High-Fat Diet Stimulated Breast Cancer Progression Via the Alteration of Macrophage Polarization Integr Cancer Ther Sep 10 2020 [PMID: 32909498] (IHC-P, Mouse) IHC-P Mouse
Li M, Guo X, Qi W et al. Macrophage polarization plays roles in bone formation instructed by calcium phosphate ceramics J. Mater. Chem. B Jan 22 2020 [PMID: 32067012]
Jia Y, Yang W, Zhang K et al. Nanofiber arrangement regulates peripheral nerve regeneration through differential modulation of macrophage phenotypes Acta Biomaterialia Oct 1 2018 [PMID: 30541701] (In Vivo, Rat) In Vivo Rat
Wei F, Liu G, Guo Y et al. Blood prefabricated hydroxyapatite/tricalcium phosphate induces ectopic vascularized bone formation via modulating the osteoimmune environment Biomater Sci Jun 22 2018 [PMID: 29931022] (IHC) IHC
Yuan XL, Zhao YP, Huang J et al. A Kv1.3 channel-specific blocker alleviates neurological impairment through inhibiting T-cell activation in experimental autoimmune encephalomyelitis CNS Neurosci Ther 2018 Mar 25 [PMID: 29577640] (Flow, Rat) Flow Rat
Boulding T, McCuaig RD, Tan A et al. LSD1 activation promotes inducible EMT programs and modulates the tumour microenvironment in breast cancer Sci Rep 2018 Jan 08 [PMID: 29311580] (ICC/IF, Human) ICC/IF Human
Show All 42 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CCR7 Antibody (NB100-712). (Showing 1 - 3 of 3 FAQs).

  1. I am curious about the isotype for product CCR7 Antibody (NB100-712).
    • NB100-712 is a goat polyclonal and it will be a mixture of isotypes.
  2. Does this product require specific antigen retrieval? I wonder if you tested the different antigen retrieval buffers and you can tell which one I should use?
    • We recommend antigen retrieval for all of our products. We recommend citrate buffer at a pH of 6.0. Here is a link to our antigen retrieval protocol.
  3. Is there a way to get this antibody without sodium azide?
    • We unfortunately do not offer our NB100-712 without sodium azide. We do offer antibody clarification kits that will remove sodium azide.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CCR7
Entrez
Uniprot