CCR7 Antibody (3D12)

Images

 
Flow Cytometry: CCR7 Antibody (3D12) [NBP1-43332] - Analysis using the PE conjugate of NBP1-43332. Analysis of human peripheral blood cells using CCR7 PE conjugate. This can be used at less than or equal to 0.5 ug per ...read more
Flow Cytometry: CCR7 Antibody (3D12) [NBP1-43332] - Analysis using the Allophycocyanin conjugate of NBP1-43332. Staining of normal human peripheral blood cells with Anti-Human CD45RA FITC and Rat IgG2a ? Isotype Control ...read more
Flow Cytometry: CCR7 Antibody (3D12) [NBP1-43332] - Analysis using the Biotin conjugate of NBP1-43332. Staining of normal human peripheral blood cells with Anti-Human CD45RA FITC and 1.0 micrograms of Rat IgG2a k ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, IHC-P
Clone
3D12
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

CCR7 Antibody (3D12) Summary

Immunogen
The immunogen for this antibody was CCR7.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
CCR7
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry < = 0.5 ug /10^6 cells in 100 ul
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This 3D12 antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. It is recommended that the staining incubation time be increased to at least 45 minutes at 4°C for optimal staining.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS, pH 7.2
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CCR7 Antibody (3D12)

  • BLR2C-C chemokine receptor type 7
  • C-C CKR-7
  • CC-CKR-7
  • CCR7
  • CCR-7
  • CD197 antigen
  • CD197
  • CDw197CC chemokine receptor 7
  • chemokine (C-C motif) receptor 7
  • CMKBR7chemokine (C-C) receptor 7
  • EBI1EBV-induced G protein-coupled receptor 1
  • EBV-induced G-protein coupled receptor 1
  • Epstein-Barr virus induced gene 1
  • Epstein-Barr virus induced G-protein coupled receptor
  • Epstein-Barr virus-induced G-protein coupled receptor 1
  • EVI1
  • lymphocyte-specific G protein-coupled peptide receptor
  • MIP-3 beta receptor

Background

The 3D12 monoclonal antibody reacts with human CCR7, also known as EBI-1 and CD197. CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of nave from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DCs. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation. Expression of CCR7 on the cell surface can be down regulated upon ligand binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

366-6C/CF
Species: Hu
NBP1-92684
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ICC/IF
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NBP1-42795
Species: Hu
Applications: WB, Flow, Func, IHC, IHC-Fr, IP, CyTOF-ready
MAB172
Species: Hu
Applications: Flow, IHC, CyTOF-ready, Neut
MAB182
Species: Hu
Applications: Flow, CyTOF-ready, ICC, Neut
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
28100002
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP2-25208
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
MAB1774
Species: Hu
Applications: Flow, CyTOF-ready, ICC

Publications for CCR7 Antibody (NBP1-43332) (0)

There are no publications for CCR7 Antibody (NBP1-43332).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for CCR7 Antibody (NBP1-43332) (0)

There are no reviews for CCR7 Antibody (NBP1-43332). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CCR7 Antibody (NBP1-43332) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional CCR7 Products

Bioinformatics Tool for CCR7 Antibody (NBP1-43332)

Discover related pathways, diseases and genes to CCR7 Antibody (NBP1-43332). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CCR7 Antibody (NBP1-43332)

Discover more about diseases related to CCR7 Antibody (NBP1-43332).
 

Pathways for CCR7 Antibody (NBP1-43332)

View related products by pathway.

PTMs for CCR7 Antibody (NBP1-43332)

Learn more about PTMs related to CCR7 Antibody (NBP1-43332).
 

Research Areas for CCR7 Antibody (NBP1-43332)

Find related products by research area.

Blogs on CCR7

There are no specific blogs for CCR7, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CCR7 Antibody (3D12) and receive a gift card or discount.

Bioinformatics

Gene Symbol CCR7
Entrez