CCR6 Overexpression Lysate (Native)

Images

 
Western Blot: CCR6 Overexpression Lysate (Adult Normal) [NBL1-08894] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CCR6.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CCR6 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC207722 and based on accession number NM_031409. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens chemokine (C-C motif) receptor 6 (CCR6), transcript variant 2, mRNA.
Gene
CCR6

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
42.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CCR6

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CCR6 Overexpression Lysate (Native)

  • BN-1
  • CC-CKR-6
  • CCR6
  • CCR-6
  • CD196 antigen
  • CD196
  • chemokine (C-C motif) receptor 6
  • CKR-L3G-protein coupled receptor 29
  • CKRL3LARC receptor
  • CMKBR6STRL22
  • DRY6
  • GPR29
  • GPR29C-C CKR-6
  • GPR-CY4
  • GPRCY4DRY-6
  • lymphocyte, 22
  • STRL22

Background

This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The gene is preferentially expressed by immature dendritic cells and memory T cells. The ligand of this receptor is macrophage inflammatory protein 3 alpha (MIP-3 alpha). This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. Alternatively spliced transcript variants that encode the same protein have been described for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) is the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for CCR6 Lysate (NBL1-08894) (0)

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Bioinformatics

Gene Symbol CCR6
Entrez