CCR6 Antibody (MM0066-3L1) - Azide and BSA Free

Immunohistochemistry-Paraffin: CCR6 Antibody (MM0066-3L1) [NBP2-12131] - Immunohistochemical analysis of CCR6 in paraffin embedded gut associated lymphoid tissue (human colon) using anti-CCR6 antibody. Image from verified customer review.

Immunohistochemistry-Paraffin: CCR6 Antibody (MM0066-3L1) [NBP2-12131] - 4% PFA fixed and parffine embedded human placental tissue section (4um) is subjected to IHC staining. Tissue was treated with 15ug/ml Protenase for antige retrieval before IHC is applied.

Product Discontinued

CCR6 Antibody (MM0066-3L1) - Azide and BSA Free Summary

• Immunogen: Human CCR6 transfected 293 cells
• Specificity: Detects human CCR6, not other CCRs
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCR6
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100-1:800
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen 1:50-1:200
  • Immunohistochemistry-Paraffin 15 ug/ml
  • Western Blot
• Application Notes: This antibody is Cytof ready.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Alternate Names for CCR6 Antibody (MM0066-3L1) - Azide and BSA Free

• BN-1
• CC-CKR-6
• CCR6
• CCR-6
• CD196 antigen
• CD196
• chemokine (C-C motif) receptor 6
• CKRL3
• CKR-L3
• CKR-L3G-protein coupled receptor 29
• CKRL3LARC receptor
• CMKBR6
• CMKBR6STRL22
• DRY6
• GPR29
• GPR29C-C CKR-6
• GPRCY4
• GPR-CY4
• GPRCY4DRY-6
• lymphocyte, 22
• STRL22

Background

CCR6 is a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein coupled receptors. It is preferentially expressed by immature dendritic cells and memory T cells. The ligand of this receptor is macrophage inflammatory protein 3 alpha (MIP 3 alpha). This receptor has been shown to be important for B lineage maturation and antigen driven B cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. CCR6 has been reported to be expressed in intestine, colon, liver, lymph node, spleen, lung, blood, umbilical cord, placenta, skin, and pancreas. It has also been reported to be expressed in lymphocytes, T cells, and B cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR6 Antibody (NBP2-12131)(1)

Publication using NBP2-12131

• Paroni M, Leccese G, Ranzani V et al. An intestinal Th17 subset is associated with inflammation in Crohn's Disease and activated by adherent-invasive Escherichia coli (AIEC) Journal of Crohn's & colitis 2023-07-18 [PMID: 37462681] (IHC-P, Human)

Review for CCR6 Antibody (NBP2-12131) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 05/20/2016

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: colon
• Species: Human
• Lot: 2015032701

FAQs for CCR6 Antibody (NBP2-12131). (Showing 1 - 1 of 1 FAQ).

1. Could you tell us whether this product can stain paraffin-embedded human tissues by Immunohistochemistry?
   • Our CCR6 antibody with catalogue number NBP2-12131 has been validated for IHC with frozen human samples, however it has not yet been tested with paraffin-embedded tissues.

Bioinformatics

• Gene Symbol: CCR6
• Uniprot:
  • P51684()