CCR6 Antibody (876515)

HEK293 human embryonic kidney cell line transfected with rat CCR6 and eGFP was stained with Mouse Anti-Rat CCR6 Monoclonal Antibody (Catalog # MAB8320) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).

• Reactivity: Rt
• Applications: Flow, CyTOF-ready
• Clone: 876515
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CCR6 Antibody (876515) Summary

• Immunogen: NSO mouse myeloma cell line transfected with rat CCR6
  Met1-Met366
  Accession # Q5BK58
• Specificity: Detects rat CCR6 in direct ELISAs.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCR6
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR6 Antibody (876515)

• BN-1
• CC-CKR-6
• CCR6
• CCR-6
• CD196 antigen
• CD196
• chemokine (C-C motif) receptor 6
• CKRL3
• CKR-L3
• CKR-L3G-protein coupled receptor 29
• CKRL3LARC receptor
• CMKBR6
• CMKBR6STRL22
• DRY6
• GPR29
• GPR29C-C CKR-6
• GPRCY4
• GPR-CY4
• GPRCY4DRY-6
• lymphocyte, 22
• STRL22

Background

CCR6 is an approximately 45 kDa G-protein linked seven transmembrane domain receptor that binds the chemokine CCL20/MIP-3 alpha . Among hematopoietic cells, CCR6 is expressed on B cells, immature dendritic cells, neutrophils, and various T cell subsets (e.g. CD4⁺ T cells, regulatory T cells, effector memory Th17 cells, and g/d T cells). It is upregulated in several cancers, Alzheimer’s brain, and the airways in obstructive pulmonary disease. CCR6 plays an important role in mucosal immunity, IgE mediated allergic reactions, immune cell infiltration into sites of inflammation, and tumor cell invasiveness. It also promotes chemotaxis and tumor angiogenesis in response to beta defensins. Rat CCR6 shares 74% and 87% amino acid sequence identity with human and mouse CCR6, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR6 Antibody (MAB8320)(2)

Publications using MAB8320

• NM Bath, X Ding, NA Wilson, BM Verhoven, BA Boldt, A Sukhwal, SR Reese, SE Panzer, A Djamali, RR Redfield Desensitization and treatment with APRIL/BLyS blockade in rodent kidney transplant model PLoS ONE, 2019-02-08;14(2):e0211865. 2019-02-08 [PMID: 30735519] (Flow Cytometry, Rat)
• Zorica Stoji?-Vuk, Ivan Pilipovi?, Ivana Vujnovi?, Mirjana Nacka-Ale, Raisa Petrovi?, Nevena Arsenovi?, Mirjana Dimitrije, Gordana Leposavi? GM-CSF-Producing Th Cells in Rats Sensitive and Resistant to Experimental Autoimmune Encephalomyelitis. PLoS ONE, 2016-11-10;0(0):1932-6203. 2016-11-10 [PMID: 27832210] (Flow Cytometry, Rat)

Bioinformatics

• Gene Symbol: CCR6
• Uniprot:
  • Q5BK58()